A Multifunctional Nanomicelle for Real-Time Targeted Imaging and Precise Near-Infrared Cancer Therapy†
Jiangwei Tian
State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
These authors contributed equally to this work.
Search for more papers by this authorDr. Lin Ding
State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
These authors contributed equally to this work.
Search for more papers by this authorCorresponding Author
Prof. Huangxian Ju
State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Huangxian Ju, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Jun-Sheng Yu, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Chaoyong James Yang, State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
Search for more papers by this authorDr. Yongchao Yang
State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Search for more papers by this authorXilan Li
State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
Search for more papers by this authorProf. Zhen Shen
State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Search for more papers by this authorDr. Zhi Zhu
State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
Search for more papers by this authorCorresponding Author
Prof. Jun-Sheng Yu
State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Huangxian Ju, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Jun-Sheng Yu, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Chaoyong James Yang, State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
Search for more papers by this authorCorresponding Author
Prof. Chaoyong James Yang
State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
Huangxian Ju, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Jun-Sheng Yu, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Chaoyong James Yang, State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
Search for more papers by this authorJiangwei Tian
State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
These authors contributed equally to this work.
Search for more papers by this authorDr. Lin Ding
State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
These authors contributed equally to this work.
Search for more papers by this authorCorresponding Author
Prof. Huangxian Ju
State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Huangxian Ju, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Jun-Sheng Yu, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Chaoyong James Yang, State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
Search for more papers by this authorDr. Yongchao Yang
State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Search for more papers by this authorXilan Li
State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
Search for more papers by this authorProf. Zhen Shen
State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Search for more papers by this authorDr. Zhi Zhu
State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
Search for more papers by this authorCorresponding Author
Prof. Jun-Sheng Yu
State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Huangxian Ju, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Jun-Sheng Yu, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Chaoyong James Yang, State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
Search for more papers by this authorCorresponding Author
Prof. Chaoyong James Yang
State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
Huangxian Ju, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Jun-Sheng Yu, State Key Laboratory of Analytical Chemistry for Life Science, State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China)
Chaoyong James Yang, State Key Laboratory of Physical Chemistry of Solid Surface, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (P.R. China)
Search for more papers by this authorThis study was supported by the National Basic Research Program (2010CB732400), the National Science Fund for Creative Research Groups (21121091 and 21021062) and the National Natural Science Foundation of China (21322506, 21005037, 21135002, 91213301, 20875045).
Abstract
Simultaneous targeted cancer imaging, therapy and real-time therapeutic monitoring can prevent over- or undertreatment. This work describes the design of a multifunctional nanomicelle for recognition and precise near-infrared (NIR) cancer therapy. The nanomicelle encapsulates a new pH-activatable fluorescent probe and a robust NIR photosensitizer, R16FP, and is functionalized with a newly screened cancer-specific aptamer for targeting viable cancer cells. The fluorescent probe can light up the lysosomes for real-time imaging. Upon NIR irradiation, R16FP-mediated generation of reactive oxygen species causes lysosomal destruction and subsequently trigger lysosomal cell death. Meanwhile the fluorescent probe can reflect the cellular status and in situ visualize the treatment process. This protocol can provide molecular information for precise therapy and therapeutic monitoring.
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