Volume 126, Issue 36 pp. 9641-9646
Zuschrift

Cell-Surface Engineering by a Conjugation-and-Release Approach Based on the Formation and Cleavage of Oxime Linkages upon Mild Electrochemical Oxidation and Reduction

Abigail Pulsipher

Abigail Pulsipher

Department of Chemistry, University of North Carolina at Chapel Hill (USA)

Department of Chemistry and Biology, York University (Canada)

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Debjit Dutta

Debjit Dutta

Department of Chemistry, University of North Carolina at Chapel Hill (USA)

Department of Chemistry and Biology, York University (Canada)

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Wei Luo

Wei Luo

Department of Chemistry, University of North Carolina at Chapel Hill (USA)

Department of Chemistry and Biology, York University (Canada)

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Prof. Muhammad N. Yousaf

Corresponding Author

Prof. Muhammad N. Yousaf

Department of Chemistry, University of North Carolina at Chapel Hill (USA)

Department of Chemistry and Biology, York University (Canada)

Department of Chemistry, University of North Carolina at Chapel Hill (USA)Search for more papers by this author
First published: 07 July 2014
Citations: 13

This research was supported by the Carolina Center for Cancer Nanotechnology Excellence (NCI), the Burroughs Wellcome Foundation (Interface Career Award), the National Science Foundation (Career Award), the National Science and Engineering Research Council of Canada (NSERC), and the Canadian Foundation for Innovation (CFI).

Abstract

We report a strategy to rewire cell surfaces for the dynamic control of ligand composition on cell membranes and the modulation of cell–cell interactions to generate three-dimensional (3D) tissue structures applied to stem-cell differentiation, cell-surface tailoring, and tissue engineering. We tailored cell surfaces with bioorthogonal chemical groups on the basis of a liposome-fusion and -delivery method to create dynamic, electroactive, and switchable cell-tissue assemblies through chemistry involving chemoselective conjugation and release. Each step to modify the cell surface: activation, conjugation, release, and regeneration, can be monitored and modulated by noninvasive, label-free analytical techniques. We demonstrate the utility of this methodology by the conjugation and release of small molecules to and from cell surfaces and by the generation of 3D coculture spheroids and multilayered cell tissues that can be programmed to undergo assembly and disassembly on demand.

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