Rapid High-Specificity microRNA Detection Using a Two-stage Isotachophoresis Assay†
Giancarlo Garcia-Schwarz
Department of Mechanical Engineering, Stanford University, 440 Escondido Mall, Building 530, Room 225, Stanford, CA 94305 (USA)
Search for more papers by this authorCorresponding Author
Prof. Juan G. Santiago
Department of Mechanical Engineering, Stanford University, 440 Escondido Mall, Building 530, Room 225, Stanford, CA 94305 (USA)
Department of Mechanical Engineering, Stanford University, 440 Escondido Mall, Building 530, Room 225, Stanford, CA 94305 (USA)Search for more papers by this authorGiancarlo Garcia-Schwarz
Department of Mechanical Engineering, Stanford University, 440 Escondido Mall, Building 530, Room 225, Stanford, CA 94305 (USA)
Search for more papers by this authorCorresponding Author
Prof. Juan G. Santiago
Department of Mechanical Engineering, Stanford University, 440 Escondido Mall, Building 530, Room 225, Stanford, CA 94305 (USA)
Department of Mechanical Engineering, Stanford University, 440 Escondido Mall, Building 530, Room 225, Stanford, CA 94305 (USA)Search for more papers by this authorWe gratefully acknowledge support from the National Science Foundation under contract number CBET-1159092. G.G.-S. is supported by a Shustek Stanford Graduate Fellowship.
Graphical Abstract
Fokus auf die Kleinen: Ein zweistufiger Assay zum Nachweis von microRNA nutzt elektrokinetische Fokussierung zur Beschleunigung der Hybridisierung und ein funktionalisiertes Hydrogel zur Affinitätsaufreinigung. Die Methode kann einzelne microRNA-Nucleotide in 15 Minuten detektieren und benötigt nur 5 ng RNA.
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