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Antiterminator-Mediated Unveiling of Cryptic Polythioamides in an Anaerobic Bacterium^†

Dipl.-Biochem. Swantje Behnken

Leibniz Institute for Natural Product Research and Infection Biology, HKI, Dept. of Biomolecular Chemistry, and Bio Pilot Plant, Beutenbergstrasse 11a, 07745 Jena (Germany)

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Dr. Thorger Lincke,

Dr. Thorger Lincke

Leibniz Institute for Natural Product Research and Infection Biology, HKI, Dept. of Biomolecular Chemistry, and Bio Pilot Plant, Beutenbergstrasse 11a, 07745 Jena (Germany)

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Dipl.-Chem. Florian Kloss,

Dipl.-Chem. Florian Kloss

Leibniz Institute for Natural Product Research and Infection Biology, HKI, Dept. of Biomolecular Chemistry, and Bio Pilot Plant, Beutenbergstrasse 11a, 07745 Jena (Germany)

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Dr. Keishi Ishida,

Dr. Keishi Ishida

Leibniz Institute for Natural Product Research and Infection Biology, HKI, Dept. of Biomolecular Chemistry, and Bio Pilot Plant, Beutenbergstrasse 11a, 07745 Jena (Germany)

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Prof. Dr. Christian Hertweck,

Corresponding Author

Prof. Dr. Christian Hertweck

[email protected]

Leibniz Institute for Natural Product Research and Infection Biology, HKI, Dept. of Biomolecular Chemistry, and Bio Pilot Plant, Beutenbergstrasse 11a, 07745 Jena (Germany)

Friedrich Schiller University, Jena (Germany)

Leibniz Institute for Natural Product Research and Infection Biology, HKI, Dept. of Biomolecular Chemistry, and Bio Pilot Plant, Beutenbergstrasse 11a, 07745 Jena (Germany)Search for more papers by this author

Dipl.-Biochem. Swantje Behnken,

Dipl.-Biochem. Swantje Behnken

Leibniz Institute for Natural Product Research and Infection Biology, HKI, Dept. of Biomolecular Chemistry, and Bio Pilot Plant, Beutenbergstrasse 11a, 07745 Jena (Germany)

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Dr. Thorger Lincke,

Dr. Thorger Lincke

Leibniz Institute for Natural Product Research and Infection Biology, HKI, Dept. of Biomolecular Chemistry, and Bio Pilot Plant, Beutenbergstrasse 11a, 07745 Jena (Germany)

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Dipl.-Chem. Florian Kloss,

Dipl.-Chem. Florian Kloss

Leibniz Institute for Natural Product Research and Infection Biology, HKI, Dept. of Biomolecular Chemistry, and Bio Pilot Plant, Beutenbergstrasse 11a, 07745 Jena (Germany)

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Dr. Keishi Ishida,

Dr. Keishi Ishida

Leibniz Institute for Natural Product Research and Infection Biology, HKI, Dept. of Biomolecular Chemistry, and Bio Pilot Plant, Beutenbergstrasse 11a, 07745 Jena (Germany)

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Prof. Dr. Christian Hertweck,

Corresponding Author

Prof. Dr. Christian Hertweck

[email protected]

Leibniz Institute for Natural Product Research and Infection Biology, HKI, Dept. of Biomolecular Chemistry, and Bio Pilot Plant, Beutenbergstrasse 11a, 07745 Jena (Germany)

Friedrich Schiller University, Jena (Germany)

First published: 27 January 2012

https://doi.org/10.1002/ange.201108214

Citations: 18

^†

We thank A. Perner for MS measurements and E. T. Papoutsakis for providing plasmid pSOS95. We are grateful to U. Knüpfer for support in fermentations. This work was supported by the “Pakt für Forschung und Innovation” of the Free State of Thuringia and the BMBF, and the International Leibniz Research School for Microbial and Biomolecular Interactions (ILRS), as part of the excellence graduate school “Jena School for Microbial Communication” (JSMC).

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Graphical Abstract

Anti-Terminator – Aufstand der Moleküle: Die Überexpression eines Antiterminator-Gens (nusG) in Clostridium cellulolyticum induziert die Biosynthese des neuartigen Antibiotikums Closthioamid und zahlreicher verwandter Thioamide. Dies ist die erste erfolgreiche genetische Manipulation eines Anaerobiers zur Aktivierung eines kryptischen Reaktionspfads. Synthetische Sonden liefern zudem Einblicke in die biogenetische Verwandtschaft der raren Thioamid-Metabolite.

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Volume124, Issue10

March 5, 2012

Pages 2475-2478

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Antiterminator-Mediated Unveiling of Cryptic Polythioamides in an Anaerobic Bacterium^†

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Antiterminator-Mediated Unveiling of Cryptic Polythioamides in an Anaerobic Bacterium^†