Zinc-Finger Proteins for Site-Specific Protein Positioning on DNA-Origami Structures†
Dr. Eiji Nakata
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011 (Japan)
CREST, JST (Japan)
Search for more papers by this authorDr. Fong Fong Liew
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011 (Japan)
Search for more papers by this authorChisana Uwatoko
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011 (Japan)
Search for more papers by this authorDr. Shigeki Kiyonaka
Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University (Japan)
CREST, JST (Japan)
Search for more papers by this authorProf. Dr. Yasuo Mori
Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University (Japan)
CREST, JST (Japan)
Search for more papers by this authorYousuke Katsuda
Department of Chemistry, Graduate School of Science, Kyoto University (Japan)
Search for more papers by this authorDr. Masayuki Endo
Institute for Integrated Cell-Material Sciences, Kyoto University (Japan)
CREST, JST (Japan)
Search for more papers by this authorProf. Dr. Hiroshi Sugiyama
Department of Chemistry, Graduate School of Science, Kyoto University (Japan)
Institute for Integrated Cell-Material Sciences, Kyoto University (Japan)
CREST, JST (Japan)
Search for more papers by this authorCorresponding Author
Prof. Dr. Takashi Morii
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011 (Japan)
CREST, JST (Japan)
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011 (Japan)Search for more papers by this authorDr. Eiji Nakata
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011 (Japan)
CREST, JST (Japan)
Search for more papers by this authorDr. Fong Fong Liew
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011 (Japan)
Search for more papers by this authorChisana Uwatoko
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011 (Japan)
Search for more papers by this authorDr. Shigeki Kiyonaka
Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University (Japan)
CREST, JST (Japan)
Search for more papers by this authorProf. Dr. Yasuo Mori
Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University (Japan)
CREST, JST (Japan)
Search for more papers by this authorYousuke Katsuda
Department of Chemistry, Graduate School of Science, Kyoto University (Japan)
Search for more papers by this authorDr. Masayuki Endo
Institute for Integrated Cell-Material Sciences, Kyoto University (Japan)
CREST, JST (Japan)
Search for more papers by this authorProf. Dr. Hiroshi Sugiyama
Department of Chemistry, Graduate School of Science, Kyoto University (Japan)
Institute for Integrated Cell-Material Sciences, Kyoto University (Japan)
CREST, JST (Japan)
Search for more papers by this authorCorresponding Author
Prof. Dr. Takashi Morii
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011 (Japan)
CREST, JST (Japan)
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011 (Japan)Search for more papers by this authorThis work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (Japan) to T.M. (No. 19021023 and 20241051).
Graphical Abstract
Fingerzeig: Zinkfingerproteine sind Adaptoren, die Positionen in DNA-Origami-Strukturen ortsselektiv ansteuern. Zwei solche Adaptoren können spezifische Positionen in orthogonaler Weise ansteuern, was in Escherichia-coli-Lysat, das Fusionsproteine der Adaptoren enthielt, zum Aufbau der erwarteten Protein-DNA-Struktur angewendet wurde.
Supporting Information
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