Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering†
Zbynek Prokop Dr.
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
These authors contributed equally to this work.
Search for more papers by this authorYukari Sato Dr.
Department of Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai (Japan)
Japan Biological Informatics Consortium, Tokyo (Japan)
These authors contributed equally to this work.
Search for more papers by this authorJan Brezovsky
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
These authors contributed equally to this work.
Search for more papers by this authorTomas Mozga
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
Search for more papers by this authorRadka Chaloupkova Dr.
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
Search for more papers by this authorTana Koudelakova
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
Search for more papers by this authorPetr Jerabek
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
Search for more papers by this authorVeronika Stepankova
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
Search for more papers by this authorRyo Natsume Dr.
Japan Biological Informatics Consortium, Tokyo (Japan)
Search for more papers by this authorJan G. E. van Leeuwen
Department of Biochemistry, University of Groningen (The Netherlands)
Search for more papers by this authorDick B. Janssen Prof.
Department of Biochemistry, University of Groningen (The Netherlands)
Search for more papers by this authorJan Florian Dr.
Department of Chemistry, Loyola University Chicago (USA)
Search for more papers by this authorYuji Nagata Dr.
Department of Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai (Japan)
Search for more papers by this authorToshiya Senda Dr.
Biomedicinal Information Research Center, National Institute of Advanced Industrial Science and Technology, Tokyo (Japan)
Search for more papers by this authorJiri Damborsky Prof.
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
Search for more papers by this authorZbynek Prokop Dr.
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
These authors contributed equally to this work.
Search for more papers by this authorYukari Sato Dr.
Department of Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai (Japan)
Japan Biological Informatics Consortium, Tokyo (Japan)
These authors contributed equally to this work.
Search for more papers by this authorJan Brezovsky
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
These authors contributed equally to this work.
Search for more papers by this authorTomas Mozga
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
Search for more papers by this authorRadka Chaloupkova Dr.
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
Search for more papers by this authorTana Koudelakova
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
Search for more papers by this authorPetr Jerabek
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
Search for more papers by this authorVeronika Stepankova
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
Search for more papers by this authorRyo Natsume Dr.
Japan Biological Informatics Consortium, Tokyo (Japan)
Search for more papers by this authorJan G. E. van Leeuwen
Department of Biochemistry, University of Groningen (The Netherlands)
Search for more papers by this authorDick B. Janssen Prof.
Department of Biochemistry, University of Groningen (The Netherlands)
Search for more papers by this authorJan Florian Dr.
Department of Chemistry, Loyola University Chicago (USA)
Search for more papers by this authorYuji Nagata Dr.
Department of Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai (Japan)
Search for more papers by this authorToshiya Senda Dr.
Biomedicinal Information Research Center, National Institute of Advanced Industrial Science and Technology, Tokyo (Japan)
Search for more papers by this authorJiri Damborsky Prof.
Loschmidt Laboratories, Department of Experimental Biology and Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno (Czech Republic), Fax. (+420) 5-4949-2556 http://loschmidt.chemi.muni.cz/peg
Search for more papers by this authorZ.P. acknowledges EMBO for financial support of his stay at the University of Groningen. Financial support is gratefully acknowledged from: the Ministry of Education, Youth, and Sports of the Czech Republic (grants LC06010 to J.D. and MSM0021622412 to Z.P.); the Grant Agency of the Czech Academy of Sciences (grant no. IAA401630901 to J.B.); the Grants-in-Aid from Ministry of Education, Culture, Sports, Science, and Technology, Japan and the Ministry of Agriculture, Forestry, and Fisheries, Japan (Y.N.); and the New Energy and Industrial Technology Development Organization (NEDO) of Japan (T.S.). Access to the METACentrum supercomputing facilities is highly appreciated (MSM6383917201). We thank Prof. Uwe Bornscheuer from the University of Greifswald for critical reading of this manuscript.
Graphical Abstract
Gezielte Mutationen der Oberflächenschleife von Halogenalkan-Dehalogenasen haben Auswirkungen auf die Enantiodiskriminierung. Die Temperaturabhängigkeit der Enantioselektivität (siehe lnE-1/T-Kurve) von β-Bromalkanen kehrt sich bei einer Deletion der Oberflächenschleife um (rote Datenpunkte), was bei Einführung einer zusätzlichen Einzelpunktmutation wieder rückgängig gemacht wird; bei α-Bromestern findet keine Änderung statt.
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