A Biocompatible Condensation Reaction for the Labeling of Terminal Cysteine Residues on Proteins†
Hongjun Ren Dr.
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorFei Xiao
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorKe Zhan
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorYoung-Pil Kim
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorHexin Xie
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorZuyong Xia
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorJianghong Rao Prof.
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorHongjun Ren Dr.
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorFei Xiao
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorKe Zhan
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorYoung-Pil Kim
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorHexin Xie
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorZuyong Xia
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorJianghong Rao Prof.
Molecular Imaging Program at Stanford, Departments of Radiology and Chemistry, Stanford University, 1201 Welch Road, Stanford, California 94305-5484 (USA), Fax: (+1) 650-736-7925 http://raolab.stanford.edu
Search for more papers by this authorThis research was supported by a grant from NIGMS (R01GM086196-01). We thank Prof. Matthew Bogyo at Stanford for access to the mass spectrometry facility.
Graphical Abstract
Lebendig markiert: Eine Proteinmarkierungsmethode, die einen einzelnen Aminosäurelinker in Form eines N-terminalen Cysteinrests sowie niedermolekulare Sonden mit einer Cyanbenzothiazol(CBT)-Einheit verwendet, wurde für die spezifische Fluoreszenzmarkierung von Proteinen in vitro und auf der Oberfläche lebender Zellen eingesetzt (siehe Schema). Diese einfache Ligationsreaktion verläuft hoch spezifisch unter physiologischen Bedingungen. Rd: ein Rhodamin-Farbstoff; TEV: Tabakmosaikvirus.
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