Oxidative metabolism in muscle mitochondria from patients with chronic alcoholism
Corresponding Author
Dr F. Cardellach MD
Department of General Internal Medicine, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
Muscle Research Unit, Servei de Medicina Interna General, Hospital Clínic i Provincial, Villarroel 170, 08036 Barcelona, SpainSearch for more papers by this authorJ. Galofré MD
Department of General Internal Medicine, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
Search for more papers by this authorJ. M. Grau MD
Department of General Internal Medicine, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
Search for more papers by this authorJ. Casademont MD
Department of General Internal Medicine, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
Search for more papers by this authorJ. B. Hoek PhD
Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, PA
Search for more papers by this authorE. Rubin MD, PhD
Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, PA
Search for more papers by this authorA. Urbano-Márquez MD
Department of General Internal Medicine, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
Search for more papers by this authorCorresponding Author
Dr F. Cardellach MD
Department of General Internal Medicine, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
Muscle Research Unit, Servei de Medicina Interna General, Hospital Clínic i Provincial, Villarroel 170, 08036 Barcelona, SpainSearch for more papers by this authorJ. Galofré MD
Department of General Internal Medicine, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
Search for more papers by this authorJ. M. Grau MD
Department of General Internal Medicine, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
Search for more papers by this authorJ. Casademont MD
Department of General Internal Medicine, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
Search for more papers by this authorJ. B. Hoek PhD
Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, PA
Search for more papers by this authorE. Rubin MD, PhD
Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, PA
Search for more papers by this authorA. Urbano-Márquez MD
Department of General Internal Medicine, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
Search for more papers by this authorAbstract
We investigated the effect of long-term ethanol intake on the function of skeletal muscle mitochondria from 30 human alcoholics. Mitochondrial studies included (1) oxidative phosphorylation, (2) the activity of the individual complexes of the respiratory chain, and (3) the cytochrome content. State 3 and state 4 oxidation rates with glutamate-malate, succinate, tetramethyl-p-phenylenediamine–ascorbate, and acetylcarnitine-malate, the activity of the individual complexes of the respiratory chain, and the concentration of mitochondrial cytochromes were comparable in control subjects and alcoholic patients, and also in myopathic and nonmyopathic alcoholic patients. These results demonstrate that alcoholic myopathy is not associated with a deficiency in mitochondrial energy supply.
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