Chapter 12
Ultrasound-Mediated Delivery Systems: Using Nano/Microbubbles or Bubble Liposomes
Kazuo Maruyama, Ryo Suzuki, Yusuke Oda, Yoko Endo-Takahashi, Yoichi Negishi,
Yoichi Negishi
Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan
Search for more papers by this authorKazuo Maruyama, Ryo Suzuki, Yusuke Oda, Yoko Endo-Takahashi, Yoichi Negishi,
Yoichi Negishi
Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan
Search for more papers by this authorBook Editor(s):Debasis Bagchi PhD, MACN, CNS, MAIChE,
Manashi Bagchi PhD, FACN,
Hiroyoshi Moriyama PhD, FACN,
Fereidoon Shahidi PhD, FACS, FAOCS, FCIC, FCIFST, FIAFoST, FIFT, FRSC,
Debasis Bagchi PhD, MACN, CNS, MAIChE
University of Houston College of Pharmacy, Houston, Texas, USA
Search for more papers by this authorManashi Bagchi PhD, FACN
NutriToday LLC, Boston, Massachusetts, USA
Search for more papers by this authorHiroyoshi Moriyama PhD, FACN
Showa Pharmaceutical University, Tokyo, Japan
Search for more papers by this authorFereidoon Shahidi PhD, FACS, FAOCS, FCIC, FCIFST, FIAFoST, FIFT, FRSC
Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland, Canada
Search for more papers by this authorSeries Editor(s): Fereidoon Shahidi PhD, FACS, FAOCS, FCIC, FCIFST, FIAFoST, FIFT, FRSC,
Fereidoon Shahidi PhD, FACS, FAOCS, FCIC, FCIFST, FIAFoST, FIFT, FRSC
Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland, Canada
Search for more papers by this authorSummary
The combination of ultrasound exposure and bubbles, including microbubbles, nanobubbles, and bubble liposomes, can be utilized to enhance drug delivery efficiency in ultrasound-mediated delivery systems. Ultrasound-induced microstreams/microjets in fluid surrounding the bubbles form transient pores in the plasma membrane through which exogenous materials such as plasmid DNA, siRNA, proteins, and/or drugs in the fluid can then enter the cell.
Cells suspended with bubbles and plasmid DNA or siRNA are exposed to ultrasound for up to a few tens of seconds to allow for transfection over a short period of time. Plasmid DNA and siRNA are directly introduced into the cytoplasm, enabling effective and rapid transfection in the presence of high serum nuclease levels. The combination of ultrasound exposure and bubbles is also a novel strategy for antigen delivery in dendritic cell (DC)-based cancer immunotherapy, thus making possible the prevention of metastasis in therapeutic models of antigen delivery into DCs.
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