Proteins Containing Nonnatural Amino Acids

Part 7. Polyamides and Complex Proteinaceous Materials
Prof. Dr. Masahiko Sisido

Prof. Dr. Masahiko Sisido

Okayama University, Department of Bioscience and Biotechnology, 3-1-1 Tsushimanaka, Okayama, Japan, 700-8530

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First published: 15 January 2005

Abstract

  • Introduction
  • Historical Outline
  • Extension of Amino Acids
  • Chemical Aminoacylation
  • Extension of Codons (Four-base Codons)
  • Adaptability of Four-base Codons in the Ribosome System and Extension to Five-base Codons
  • Nonnatural Base Pairs for Codon Extension
  • In-vitro Protein Synthesis in the Presence of tRNAs that are Amino-acylated with Nonnatural Amino Acids
  • Purification and Identification of Nonnatural Mutants
  • Multiple Incorporation of Nonnatural amino acids by using Different Orthogonal Four-base Codons
  • Mutants that Contain a Single Nonnatural Amino Acid at Random Positions
  • Combination of In-vitro Synthesis and Chemical Synthesis for Screening Nonnatural Mutants and for Large-scale Production
  • In-vivo Synthesis of Nonnatural Mutants
  • Examples of Specialty Functions of Nonnatural Mutants
    • Fluorescence Labeling
    • Electron Transfers Inside Protein Frameworks
  • Outlook and Perspectives
  • Acknowledgments

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