MALDI-MS Characterization of Maltooligo/polysaccharides From Debranched Starch Amylopectin of Corn and Barley
Jian Wang
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada, T6G 2P5
Search for more papers by this authorGaosong Jiang
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada, T6G 2P5
Search for more papers by this authorThava Vasanthan
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada, T6G 2P5
Search for more papers by this authorPeter Sporns
Search for more papers by this authorJian Wang
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada, T6G 2P5
Search for more papers by this authorGaosong Jiang
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada, T6G 2P5
Search for more papers by this authorThava Vasanthan
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada, T6G 2P5
Search for more papers by this authorPeter Sporns
Search for more papers by this authorAbstract
Aprotocol was developed for molecular characterization of debranched starch amylopectin using Matrix-assisted laser desorption/ionizationmass spectrometry (MALDI-MS). Starch from waxy corn was used in the protocol development. Various factors influencing the protocol were investigated and discussed. Using the developed protocols, starch amylopectin from barley grains (waxy-type Candle and regular-type Phoenix) were also characterized. The study suggested that MALDI-MS is suitable to determine the molecular size profile of debranched starch (maltooligo- and polysaccharides) faster and with greater accuracy than any present analytical methodology.
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