Research Paper
Production of Leucrose by Dextransucrase
Klaus Buchholz,
Martina Noll-Borchers,
Dieter Schwengers,
Klaus Buchholz
Search for more papers by this authorMartina Noll-Borchers
Lehrstuhl für Technologie der Kohlenhydrate, Technische Universität, Virchowstr. 2, 45470 Mülheim (Germany)
Search for more papers by this authorDieter Schwengers
Lehrstuhl für Technologie der Kohlenhydrate, Technische Universität, Liesentorweg 12, 47802 Krefeld (Germany)
Search for more papers by this authorKlaus Buchholz,
Martina Noll-Borchers,
Dieter Schwengers,
Klaus Buchholz
Search for more papers by this authorMartina Noll-Borchers
Lehrstuhl für Technologie der Kohlenhydrate, Technische Universität, Virchowstr. 2, 45470 Mülheim (Germany)
Search for more papers by this authorDieter Schwengers
Lehrstuhl für Technologie der Kohlenhydrate, Technische Universität, Liesentorweg 12, 47802 Krefeld (Germany)
Search for more papers by this authorFirst published: 23 December 1998
Citations: 22
Abstract
Dextransucrase can be used to synthesize the disaccharide leucrose from sucrose and fructose under appropriate reaction conditions. HPLC methods to analyze the reaction solutions and an enzyme activity test were developed to determine reaction kinetics and by-products in detail. As a result, a separate acceptor binding site at the enzyme was postulated to explain all aspects of dextransucrase catalyzed reactions. The unconventional immobilization method for dextransucrase by entrapment in calcium alginate beads showed good results concerning activity, efficiency and operational stability. The immobilization mechanism was investigated and the reaction conditions for leucrose formation were optimized. A surprising aspect was the finding, that yields and selectivity were much improved by reactions in ice. Referring to technical application of leucrose formation, the productivity of a continuous process proved to be optimal in a tubular reactor; it compares favorably with a discontinuous use of the immobilized enzyme.
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