Shigella and Listeria induce membrane protrusions, now called transpodia, to transport the bacteria from cell to cell. Myosin-10, an unconventional myosin known to play a critical role in the formation of host cell filopodia is now shown to also play an integral role in Shigella- and Listeria-induced transpodia formation. Knock-down of Myosin-10 markedly reduces plaque formation in confluent cell monolayers, and enhancement of transpodia formation requires the binding of the myosin head domain to actin filaments along the sides of the bacterium, and the binding of the myosin tail PH domain to the peripheral membrane.