journal menu
![[Figure 5]](/d/issues/2025/08/00/ic5124/ic5124fig5mag.jpg)
|
| Figure 5 Separating conformational/compositional states in SIMPLE ab initio. (a) Starting with the cleaned FliPQRFlhB data set and reconstructing two volumes (green semi-transparent surface and silver solid surface) separates particles with lower occupancy of the FlhB component (highlighted with a black ellipse) and less order in the intra-helix loops at the top (highlighted with asterisks). (b) Multi-volume reconstruction of TRPM4 separates particles in which the N-terminal domains and C-terminal coiled coil are well ordered (silver, semi-transparent surface) and where these are not well ordered (blue surface). (c) and (d) compare the three `good' ribosome reconstructions obtained directly from the stream (see Fig. 3 ![[link]](https://journals.iucr.org/logos/arrows/d_arr.gif){kind=small} ) and reveals that (c) states 1 and 4 are conformationally consistent (state 1 shown as a silver surface,
with difference density calculated in ChimeraX overlaid as a lilac surface) and that (d) states 2 and 1 differ in the orientation of the small ribosomal subunit with respect
to the large ribosomal subunit (state 1 shown as a silver surface, with difference
density calculated in ChimeraX overlaid as a blue surface). The arrows indicate the major movement of the small
ribosomal subunit between the two volumes overlaid on the large ribosomal subunit.
(e) Three states generated by multi-volume ab initio 3D reconstruction on cleaned RNA polymerase data. (f) One state is shown in silver with the difference density for the other two states
shown colored as in (e). |
![[Figure 5]](https://journals.iucr.org/10.1107/S2059798325005686/ic5124fig5.jpg)
 |
STRUCTURAL BIOLOGY |
ISSN: 2059-7983
access
