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![[Figure 1]](https://journals.iucr.org/jc5046fig1mag.jpg)
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| Figure 1 Conservation of key residues in BAM2 and BAM7 from flowering plants and how they compare with the corresponding residues in DF-BAM and BAM1, and a map of the corn BAM7 gene with its two predicted transcripts. (a) WebLogo illustrating the conservation of residues predicted to be part of the nuclear localization signal in the DNA-binding domain as identified by mutagenesis in A. thaliana BAM7 (Reinhold et al., 2011  ). The alignment included 14 species of flowering plants that contain BAM7 that were
selected to represent a diversity of orders and 12 species that contain DF-BAM7 (see
Supplementary Table S1 for species and accession numbers). (b) WebLogo illustrating the conservation of 15 residues in the same species as in (a) identified as forming hydrogen bonds to starch in the active site of soybean (Glycine max) BAM5 (Laederach et al., 1999). (c) WebLogo illustrating the conservation of residues as in (a) compared with the three residues identified in BAM2 as being involved in allosteric
regulation of activity in BAM2 (Ser464) or starch binding to a surface binding site
for starch (Gly335, Gly446 and Trp449) (Monroe et al., 2017, 2018). (d) WebLogo illustrating the conservation of three residues in the same species as in
(a) that were identified by mutagenesis as being important for tetramer stabilization:
Trp456 and Asp590 in interface `A' and Phe238 in interface `B' (Monroe et al., 2018). (e) Predicted dual-function ZmBAM7 gene model. Coding regions of exons are colored black, with the exception of a region
unique to the N-terminus of ZmBAM7-S that is colored red. The locations of the two
putative transcriptional start sites (TSS1 and TSS2) and their respective translational
start sites (AUG) in the two predicted transcripts are indicated with arrows. The
5′ and 3′ untranslated regions (UTR) of both transcripts are colored white. |
![[Figure 1]](https://journals.iucr.org/10.1107/S2059798322002169/jc5046fig1.jpg)
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STRUCTURAL BIOLOGY |
ISSN: 2059-7983
