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Figure 2 (a) Structural overlay of P10VF-Lig (blue) with T4 DNA ligase (yellow), highlighting the relatively more extensive surface of the T4 enzyme. DNA in this view (grey) is shown for the P10VF-Lig structure only, but is essentially identical to the T4 DNA ligase structure when superimposed. (b) Structural overlay of P10VF-Lig with T4 DNA ligase for individual domains rendered in cartoon mode to highlight secondary-structural elements. Features which are present in T4 DNA ligase but absent in P10VF-Lig are coloured red. DNA adenylate is shown for the P10VF-Lig structure only. |