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Stable Expression and Regulation of a Rat Brain K⁺ Channel

Corresponding Author

Stuart D. Critz

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, U.S.A.

Address correspondence and reprint requests to Dr. S. D. Critz at Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, U.S.A.Search for more papers by this author

Barbara A. Wible,

Barbara A. Wible

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, U.S.A.

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Hector S. Lopez,

Hector S. Lopez

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, U.S.A.

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Arthur M. Brown,

Arthur M. Brown

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, U.S.A.

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Stuart D. Critz,

Corresponding Author

Stuart D. Critz

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, U.S.A.

Barbara A. Wible,

Barbara A. Wible

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, U.S.A.

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Hector S. Lopez,

Hector S. Lopez

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, U.S.A.

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Arthur M. Brown,

Arthur M. Brown

Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, U.S.A.

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First published: March 1993

https://doi.org/10.1111/j.1471-4159.1993.tb03273.x

Citations: 54

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Abstract

Abstract: The Shaw-type K⁺ channel K_v3.1 was stably transfected in human embryonic kidney cells. Voltage dependence of activation, K⁺ permeability, sensitivity to external tetraethylammonium, and unitary conductance were similar to K_v 3.1 channels expressed transiently in Xenopus oocytes. K_v 3.1 channels appear to be regulated because the protein kinase C activator phorbol 12,13-dibutyrate decreased K_v 3.1 currents. Based on these results, we find that the stable expression of voltage-gated K⁺ channels in human embryonic kidney cells appears to be well suited for analysis of both biophysical and biochemical regulatory processes.

Abbreviations

DMSO: dimethyl sulfoxide
PDBu: phorbol 12,13-dibutyrate
PKC: protein kinase C
TEA: tetraethylammonium

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Volume60, Issue3

March 1993

Pages 1175-1178

Stable Expression and Regulation of a Rat Brain K⁺ Channel

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Stable Expression and Regulation of a Rat Brain K⁺ Channel