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Ontogenetic Profile of the Adenosine Uptake Sites in Rat Forebrain
P. F. Morgan,
P. Montgomery,
P. J. Marangos,
P. F. Morgan
Unit on Neurochemistry, Biological Psychiatry Branch, National Institute of Mental Health, Bethesda, Maryland, U.S.A.
Search for more papers by this authorP. Montgomery
Unit on Neurochemistry, Biological Psychiatry Branch, National Institute of Mental Health, Bethesda, Maryland, U.S.A.
Search for more papers by this authorCorresponding Author
P. J. Marangos
Unit on Neurochemistry, Biological Psychiatry Branch, National Institute of Mental Health, Bethesda, Maryland, U.S.A.
Address correspondence and reprint requests to Dr. P. J. Marangos at Unit on Neurochemistry, Biological Psychiatry Branch, National Institute of Mental Health, 9000 Rockville Pike, Building 10, Room 3C-210, Bethesda, MD 20892, U.S.A.Search for more papers by this authorP. F. Morgan,
P. Montgomery,
P. J. Marangos,
P. F. Morgan
Unit on Neurochemistry, Biological Psychiatry Branch, National Institute of Mental Health, Bethesda, Maryland, U.S.A.
Search for more papers by this authorP. Montgomery
Unit on Neurochemistry, Biological Psychiatry Branch, National Institute of Mental Health, Bethesda, Maryland, U.S.A.
Search for more papers by this authorCorresponding Author
P. J. Marangos
Unit on Neurochemistry, Biological Psychiatry Branch, National Institute of Mental Health, Bethesda, Maryland, U.S.A.
Address correspondence and reprint requests to Dr. P. J. Marangos at Unit on Neurochemistry, Biological Psychiatry Branch, National Institute of Mental Health, 9000 Rockville Pike, Building 10, Room 3C-210, Bethesda, MD 20892, U.S.A.Search for more papers by this authorAbstract
Abstract: The ontogenesis of rat forebrain adenosine uptake sites labelled by [3H]nitrobenzylthioinosine ([3H]NBI) was determined and compared to that of rat forebrain adenosine receptors labelled by N6-cyclohexyl[3H]adenosine ([3H]-CHA). [3H]NBI binding is highly invariant with similar levels of [3H]NBI binding sites from embryonic day 19 to day 30 postpartum. Scatchard and Hill analyses reveal the binding of [3H]NBI in 6-day-old tissue to be indistinguishable from such binding in 30-day-old tissue. In contrast, [3H]-CHA binding is highly variant. [3H]CHA binding develops slowly but steadily from about embryonic day 19, with adult binding levels being achieved at around 25 days postpartum. The ontogenetic profile of [3H]CHA appears to coincide with synaptogenesis whereas that of [3H]NBI does not.
Abbreviations used:
-
- 3H]CHA
-
- N 6-cyclohexyl[3H]adenosine
-
- [3H]NBI
-
- [3H]nitrobenzylthioinosine
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