Volume 51, Issue 9 pp. 833-840

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Molecular Epidemiology of Rabies in Vietnam

Junpei Yamagata,

Junpei Yamagata

Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, Yufu, Oita, 879-5593 Japan

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Kamruddin Ahmed,

Kamruddin Ahmed

Division of Infectious Diseases, Department of Social and Environmental Medicine, Institute of Scientific Research, Yufu, Oita, 879-5593 Japan

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Pakamatz Khawplod,

Pakamatz Khawplod

Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, Yufu, Oita, 879-5593 Japan

Queen Saovabha Memorial Institute, Thai Red Cross Society, Rama IV Rd., Bangkok, 10330 Thailand

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Kazuaki Mannen,

Kazuaki Mannen

Laboratory Animal Research Center, Faculty of Medicine, Oita University, Yufu, Oita, 879-5593 Japan

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Dinh Kim Xuyen,

Dinh Kim Xuyen

National Institute of Hygiene and Epidemiology, Yersin Street Hanoi, Vietnam

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Huynh Huu Loi,

Huynh Huu Loi

The Sub Department of Animal Health of Ho Chi Minh City 11, Vietnam

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Nguyen Van Dung,

Nguyen Van Dung

The Sub Department of Animal Health of Ho Chi Minh City 11, Vietnam

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Akira Nishizono,

Corresponding Author

Akira Nishizono

Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, Yufu, Oita, 879-5593 Japan

Address correspondence to Dr. Akira Nishizono, Division of Microbiology, Department of Infectious Diseases, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan. Fax: +81-97-586-5719. E-mail: [email protected]Search for more papers by this author

Junpei Yamagata,

Junpei Yamagata

Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, Yufu, Oita, 879-5593 Japan

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Kamruddin Ahmed,

Kamruddin Ahmed

Division of Infectious Diseases, Department of Social and Environmental Medicine, Institute of Scientific Research, Yufu, Oita, 879-5593 Japan

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Pakamatz Khawplod,

Pakamatz Khawplod

Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, Yufu, Oita, 879-5593 Japan

Queen Saovabha Memorial Institute, Thai Red Cross Society, Rama IV Rd., Bangkok, 10330 Thailand

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Kazuaki Mannen,

Kazuaki Mannen

Laboratory Animal Research Center, Faculty of Medicine, Oita University, Yufu, Oita, 879-5593 Japan

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Dinh Kim Xuyen,

Dinh Kim Xuyen

National Institute of Hygiene and Epidemiology, Yersin Street Hanoi, Vietnam

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Huynh Huu Loi,

Huynh Huu Loi

The Sub Department of Animal Health of Ho Chi Minh City 11, Vietnam

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Nguyen Van Dung,

Nguyen Van Dung

The Sub Department of Animal Health of Ho Chi Minh City 11, Vietnam

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Akira Nishizono,

Corresponding Author

Akira Nishizono

Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, Yufu, Oita, 879-5593 Japan

First published: 14 November 2013

https://doi.org/10.1111/j.1348-0421.2007.tb03979.x

Citations: 24

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Abstract

The present study was done to determine the molecular epidemiology of rabies virus (RV) in Vietnam. The nucleoprotein (N) and glycoprotein (G) genes of RVs were amplified from the brains of ten rabid dogs of Ho Chi Minh City, Vietnam. The nucleotide sequences of these genes were compared with those of other Asian strains to find the possible relationship among them. Phylogenetic analysis revealed that the Asian N gene segregated into three main branches, namely South-East Asia 1 (SEA 1), South-East Asia 2 (SEA 2) and Indian subcontinent (ISC) genotypes. The SEA 1 genotype comprised RVs from Malaysia, Vietnam and Thailand. The SEA 2 genotype contained strains from the Philippines, and the ISC genotype comprised strains from Sri Lanka and India. Phylogenetically G genes of RVs from Vietnam and Thailand were clustered together. Our study suggests that Vietnamese and Thai RVs are closely related and might have originated from a common ancestor.

Abbreviations

cDNA: complementary deoxyribonucleic acid
G: glycoprotein
HCM: Ho Chi Minh City
IPTG: isopropyl-β-D-thiogalactopyranoside
MMLV: Moloney Murine Leukemia Virus
N: nucleoprotein
RT-PCR: reverse transcription and polymerase chain reaction
RV: rabies virus
SEA 1: South-East Asia 1
SEA 2: South-East Asia 2
ISC: Indian subcontinent.

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Volume51, Issue9

September 2007

Pages 833-840

Molecular Epidemiology of Rabies in Vietnam

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Molecular Epidemiology of Rabies in Vietnam

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