Volume 14, Issue 11 pp. 2595-2606
Original Article
Open Access

Islet Product Characteristics and Factors Related to Successful Human Islet Transplantation From the Collaborative Islet Transplant Registry (CITR) 1999–2010

A. N. Balamurugan

Corresponding Author

A. N. Balamurugan

Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN

† Current address: Islet Cell Laboratory, Cardiovascular Innovation Institute, Department of Surgery, University of Louisville, Louisville, KYCorresponding authors: Appakalai N. Balamurugan, [email protected]and Franca B. Barton, [email protected]Search for more papers by this author
B. Naziruddin

B. Naziruddin

Baylor Annette C. and Harold C. Simmons Transplant Institute, Dallas, TX

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A. Lockridge

A. Lockridge

Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN

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M. Tiwari

M. Tiwari

Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN

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G. Loganathan

G. Loganathan

Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN

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M. Takita

M. Takita

Baylor Annette C. and Harold C. Simmons Transplant Institute, Dallas, TX

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S. Matsumoto

S. Matsumoto

Research and Development Center, Otsuka Pharmaceutical Factory, Inc., Tokushima, Japan

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K. Papas

K. Papas

Institute for Cellular Transplantation, University of Arizona, Tucson, AZ

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M. Trieger

M. Trieger

The EMMES Corporation, Rockville, MD

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H. Rainis

H. Rainis

The EMMES Corporation, Rockville, MD

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T. Kin

T. Kin

Clinical Islet Laboratory, University of Alberta, Edmonton, AB

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T. W. Kay

T. W. Kay

St. Vincent's Hospital, Melbourne, Australia

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S. Wease

S. Wease

The EMMES Corporation, Rockville, MD

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S. Messinger

S. Messinger

Department of Public Health Services, University of Miami, Miami, FL

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C. Ricordi

C. Ricordi

Diabetes Research Institute, University of Miami, Miami, FL

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R. Alejandro

R. Alejandro

Diabetes Research Institute, University of Miami, Miami, FL

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J. Markmann

J. Markmann

Department of Surgery, Massachusetts General Hospital, Boston, MA

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J. Kerr-Conti

J. Kerr-Conti

Lille University Hospital, Lille, France

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M. R. Rickels

M. R. Rickels

Department of Medicine, University of Pennsylvania, Philadelphia, PA

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C. Liu

C. Liu

Department of Surgery, University of Pennsylvania, Philadelphia, PA

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X. Zhang

X. Zhang

Feinberg School of Medicine, Northwestern University, Chicago, IL

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P. Witkowski

P. Witkowski

Department of Surgery, University of Chicago, Chicago, IL

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A. Posselt

A. Posselt

Department of Surgery, University of California, San Francisco, San Francisco, CA

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P. Maffi

P. Maffi

Vita-Salute, San Raffaele University, Milan, Italy

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A. Secchi

A. Secchi

Vita-Salute, San Raffaele University, Milan, Italy

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T. Berney

T. Berney

Department of Surgery, Geneva University Hospital, Geneva, Switzerland

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P. J. O'Connell

P. J. O'Connell

National Pancreas Transplant Unit, University of Sydney at Westmead Hospital, Sydney, Australia

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B. J. Hering

B. J. Hering

Schulze Diabetes Institute, Department of Surgery, University of Minnesota, Minneapolis, MN

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F. B. Barton

Corresponding Author

F. B. Barton

The EMMES Corporation, Rockville, MD

Corresponding authors: Appakalai N. Balamurugan, [email protected]and Franca B. Barton, [email protected]Search for more papers by this author
First published: 02 October 2014
Citations: 135

Abstract

The Collaborative Islet Transplant Registry (CITR) collects data on clinical islet isolations and transplants. This retrospective report analyzed 1017 islet isolation procedures performed for 537 recipients of allogeneic clinical islet transplantation in 1999–2010. This study describes changes in donor and islet isolation variables by era and factors associated with quantity and quality of final islet products. Donor body weight and BMI increased significantly over the period (p < 0.001). Islet yield measures have improved with time including islet equivalent (IEQ)/particle ratio and IEQs infused. The average dose of islets infused significantly increased in the era of 2007–2010 when compared to 1999–2002 (445.4 ± 156.8 vs. 421.3 ± 155.4 ×103 IEQ; p < 0.05). Islet purity and total number of β cells significantly improved over the study period (p < 0.01 and <0.05, respectively). Otherwise, the quality of clinical islets has remained consistently very high through this period, and differs substantially from nonclinical islets. In multivariate analysis of all recipient, donor and islet factors, and medical management factors, the only islet product characteristic that correlated with clinical outcomes was total IEQs infused. This analysis shows improvements in both quantity and some quality criteria of clinical islets produced over 1999–2010, and these parallel improvements in clinical outcomes over the same period.

Abbreviations

  • BMI
  • body mass index
  • CIT
  • cold ischemia time
  • CITR
  • Collaborative Islet Transplant Registry
  • HTK
  • histidine-tryptophan-ketoglutarate
  • IAK
  • islet after kidney transplantation
  • IEQ
  • islet equivalent
  • ITA
  • islet transplant alone
  • JDRF
  • Juvenile Diabetes Research Foundation
  • NIDDK
  • National Institute of Diabetes and Digestive and Kidney Diseases
  • SIK
  • simultaneous islet and kidney transplantation
  • TLM
  • two layer method
  • UW
  • University of Wisconsin solution
  • Introduction

    Over the last two decades, numerous studies have attempted to document the donor and procedural factors that significantly influence the success of pancreatic islet isolation and clinical transplantation 1-12. The following donor variables are commonly identified in those studies: donor age, BMI, metabolic condition, pancreas characteristics, cause of death and cold ischemia time (CIT). Various evaluations of the procedures have been performed including processing times, pancreas preservation method, digestion enzyme selection and purification method. The most frequently used primary outcome in these studies pertained to islet yield as represented by total islet equivalent (IEQ) and IEQ/g pancreas or IEQ/kg of recipient body weight. These reports have laid a nearly unanimous emphasis on the importance of reaching a critical minimum islet mass to achieve transplant success.

    Consistent results across the studies have been observed for some variables, such as age and BMI; however, controversial findings also have been reported 12, 13. The lack of a consistent or sufficiently detailed reporting of variables and outcome measures limits the efficacy of meta-analysis to resolve these debates. In addition, recent investigations have begun to highlight the importance of nonyield-based indicators in predicting the ultimate goal of any islet transplantation procedure, normalization of blood sugar control and a relief from diabetic symptoms in the transplant recipients 4, 10, 11.

    The current investigation represents a comprehensive review of islet characteristics, analyzing data from 1017 clinical islet isolations conducted in North American and Juvenile Diabetes Research Foundation (JDRF) European and Australian centers through the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) - and JDRF-sponsored Collaborative Islet Transplant Registry (CITR) database. The CITR has collected data on donors; pancreas procurement, isolation and product characteristics; and baseline and follow-up on recipients of human allogeneic islet transplants from 1999 to the present. CITR operates with all sites and the Coordinating Center undergoing annual review of the research protocol by local Internal Review Boards. This collaboration, concomitant with the standardization of variable and outcome reporting, has produced the largest and most thorough collection on the production of human clinical-grade islets. We focus in this report on identifying the factors that significantly impacted the yield, purity and endocrine function of the final islet product and clinical outcomes, while recipient and management characteristics are reserved for a separate analysis.

    Methods

    This analysis comprises data from 1017 islet transplant alone (ITA), islet after kidney (IAK) and simultaneous islet and kidney (SIK) allogeneic transplants reported to CITR from 1999 to 2010. The analysis period was divided into 4-year eras: 1999–2002, 2003–2006, 2007–2010 as reported previously for clinical outcomes 14. For multiple-donor infusions given on the same day, the donor, pancreas and procurement characteristics were averaged or summed as appropriate to characterize the composite infused preparation.

    Table 1A organizes variables according to donor characteristics, procurement and processing characteristics, islet product characteristics and infusion characteristics. Data shown in Table 1D on islet product characteristics comprises the primary outcomes of this analysis, while the others are investigated as possible factors associated with these outcomes. The variables themselves may vary according to calendar year of transplant (era), continent (North America vs. the JDRF sites in Europe and Australia), ITA versus IAK/SIK and many other factors that may or may not be captured in the data. Categorical variables and outcomes are described by their distribution: in particular many are characterized as either present (1) or absent (0); those comprising several distinct levels, including preservation solution, enzyme and gradient type, are reported and analyzed as class variables. Continuous variables are described by their mean and standard deviation. Not all variables were available for all preparations. Table 1A shows the distribution of available data for each variable, excluding the missing data (i.e. treated as missing at random); hence, the percentage distributions are estimates of the actual distribution.

    Associations between the variables and the outcomes were estimated by general linear models, first univariately, to identify all associations at p < 0.05, then by step-down multivariate modeling to isolate net effects after adjustment for confounders, with each islet infusion as an independent observation. For about 10% of all “infusions” islet products were derived from two to three donors administered on the same day; these are analyzed as composite infusions because clinical outcomes cannot be ascribed to one or the other donor products, but to the infusion of multiple products given on the same day. All variables in CITR including recipient and donor characteristics, islet procurement, processing and final product criteria, as well as medical management variables, were evaluated by comprehensive multivariate models for their association with clinical outcomes of islet transplantation including achievement and retention of insulin independence (≥14 days, but usually ranging many months or years), and retention of fasting C-peptide >0.3 ng/mL. These results are reported in the CITR 7th Annual Report 15, also based on the same data set as the present report. SAS V9.3 (SAS Institute, Cary, NC) was used for all computations. Definitions of the terminology shown in the tables and figures have been described in the annual report of CITR 15.

    Results

    There were sufficient data available to report on 1017 islet preparations used for clinical allogeneic transplantation. These 1017 islet preparations were infused into 537 recipients: 167 (31%) patients received single infusion, 270 (50%) received two infusions, 92 (17%) received three infusions, 7 (1%) received four infusions, and 1 patient received six infusions. For each infusion event, a single donor pancreas was used 90% of the time, two pancreata were pooled for 9.3% of infusions and islets from three donors were combined for a single infusion in five instances.

    Changes in donor and islet isolation by era

    Table 1A shows the various characteristics of the 1017 donors and infusions, organized for comparison by era of 1999–2002, 2003–2006 and 2007–2010. Significant increase in body weight, BMI, use of steroid and insulin during treatment at last admission use were observed among donor characteristics (p < 0.001, <0.001, <0.05 and <0.05, Table 1A). Pancreas preservation method significantly changed during the study period (p < 0.001, Table 1B); the ratio of University of Wisconsin solution (UW) only and two layer method (TLM) decreased in 2007–2010 compared to the earlier eras (p < 0.001, Table 1B). Time from death to cross-clamp, time from pancreas recovery to transplant and death to transplant were significantly prolonged (p < 0.001, Table 1B).

    Table 1A. Donor characteristics
    Total (n = 1017) Era p-Value
    1999–2002 (n = 257) 2003–2006 (n = 472) 2007–2010 (n = 288)
    Age (years) 43.2 ± 12.2 (n = 846) 42.5 ± 12.2 43.3 ± 11.7 43.6 ± 13
    Gender, % (n)
    Female donor only 37.3 (n = 379) 38.9 (n = 100) 36.9 (n = 174) 36.5 (n = 105)
    Mixed-gender multiple-donor infusion 3.7 (n = 38) 5.4 (n = 14) 4.7 (n = 22) 0.7 (n = 2)
    Male donor only 59 (n = 600) 55.6 (n = 143) 58.5 (n = 276) 62.8 (n = 181)
    Body weight (kg) 87.6 ± 20.5 (n = 946) 84.1 ± 20.6 86.6 ± 19.8 93.5 ± 20.7 <0.001
    Height (cm) 173.4 ± 9.8 (n = 994) 172.5 ± 9 173.5 ± 9.9 174 ± 10.3
    BMI (kg/m2) 29.1 ± 6.3 (n = 944) 28.3 ± 6.5 28.7 ± 6.2 30.9 ± 6.1 <0.001
    Ethnicity, % (n)
    Non-Hispanic 90.9 (n = 538) 92.9 (n = 156) 89 (n = 251) 92.3 (n = 131)
    Mixed-ethnicity multiple-donor infusion 1.9 (n = 11) 2.4 (n = 4) 2.5 (n = 7)
    Hispanic 7.3 (n = 43) 4.8 (n = 8) 8.5 (n = 24) 7.7 (n = 11)
    Donor race, % (n)
    White 89.8 (n = 564) 93.9 (n = 168) 87.5 (n = 273) 89.8 (n = 123)
    Cause of death, % (n)
    Trauma 33.2 (n = 308) 32.9 (n = 76) 32 (n = 145) 35.8 (n = 87)
    Cerebrovascular/stroke 58.4 (n = 541) 55.8 (n = 129) 61.1 (n = 277) 55.6 (n = 135)
    History, % (n)
    Hypertension 35.2 (n = 294) 36.7 (n = 65) 35.1 (n = 150) 34.2 (n = 79)
    Alcohol use 18.6 (n = 145) 13.9 (n = 25) 22.2 (n = 91) 15.2 (n = 29)
    Laboratory results
    Creatinine (mg/dL) 1.1 ± 0.6 (n = 809) 1.1 ± 0.8 1.1 ± 0.6 1 ± 0.5
    Bilirubin (mg/dL) 0.9 ± 0.7 (n = 633) 0.9 ± 0.7 0.9 ± 0.6 0.9 ± 0.8
    AST (U/L) 76.7 ± 202 (n = 655) 115.7 ± 342.1 63.1 ± 128.9 70.9 ± 154.5
    ALT (U/L) 63.4 ± 165.1 (n = 720) 89.3 ± 286 52.3 ± 107.6 63.6 ± 127.2
    Lipase (U/L) 1.1 ± 1.9 (n = 631) 1 ± 1.7 1.1 ± 1.9 1 ± 2.2
    Amylase (U/L) 2.6 ± 5.1 (n = 726) 2.3 ± 3.7 2.5 ± 4.2 3.4 ± 8
    Preinsulin glucose (mg/dL) 125.5 ± 38.2 (n = 743) 129.5 ± 36 123.3 ± 38.5 126.1 ± 39.8
    Maximum blood glucose (mg/dL) 230.6 ± 83.5 (n = 773) 247 ± 98 228.7 ± 84.2 221.7 ± 67.5
    Max-Min glucose (mg/dL) 110.7 ± 86 (n = 670) 119.4 ± 98.6 109.5 ± 84.3 104.4 ± 75.1
    HbA1c (%) 5.5 ± 0.5 (n = 161) 5.3 ± 0.4 5.4 ± 0.5 5.5 ± 0.5
    Treatment at last admission, % (n)
    Vasopressors 96.7 (n = 816) 96.7 (n = 204) 97.7 (n = 432) 94.2 (n = 180)
    Steroids 59 (n = 309) 65.5 (n = 78) 52 (n = 157) 71.8 (n = 74) <0.05
    Insulin 42.6 (n = 321) 27.8 (n = 49) 43 (n = 166) 55.2 (n = 106) <0.05
    Transfusion prerecovery 32.9 (n = 244) 34.8 (n = 65) 33.7 (n = 134) 28.8 (n = 45)
    Transfusion intra-operatively 6.5 (n = 41) 9.1 (n = 15) 5.7 (n = 20) 5.1 (n = 6)
    Table 1B. Pancreas preservation and recovery
    Total (n = 1017) Era p-Value
    1999–2002 (n = 257) 2003–2006 (n = 472) 2007–2010 (n = 288)
    Procurement/transplant centers related, % (n) 61.5 (n = 551) 60.6 (n = 157) 61.3 (n = 284) 63.2 (n = 110)
    Pancreas preservation, % (n) <0.001
    UW only 42.3 (n = 430) 58.4 (n = 150) 46 (n = 217) 21.9 (n = 63)
    TLM only 20.4 (n = 207) 16.7 (n = 43) 29.2 (n = 138) 9 (n = 26)
    HTK only 7 (n = 71) 0 (n = 0) 6.8 (n = 32) 13.5 (n = 39)
    Celsior 2.3 (n = 23) 1.9 (n = 5) 1.7 (n = 8) 3.5 (n = 10)
    UW+TLM 3.8 (n = 39) 3.9 (n = 10) 4.7 (n = 22) 2.4 (n = 7) <0.001
    Combinations/other 4.8 (n = 49) 4.7 (n = 12) 4 (n = 19) 6.3 (n = 18)
    Missing/unknown 19.5 (n = 198) 14.4 (n = 37) 7.6 (n = 36) 43.4 (n = 125)
    Time (hours)
    Admission to brain death 50.3 ± 61.3 (n = 592) 50 ± 57.3 51.4 ± 66.8 47.9 ± 51.1
    Death to cross-clamp 18.4 ± 8.3 (n = 766) 16.4 ± 6.6 18.8 ± 8.6 19.8 ± 9.2 <0.001
    Cross-clamp to pancreas recovery 0.8 ± 1.1 (n = 627) 0.6 ± 0.4 0.9 ± 1.4 0.9 ± 0.5
    Death to pancreas recovery 18.7 ± 8.6 (n = 571) 16.6 ± 6.9 19.2 ± 8.9 19.7 ± 9.2
    Cold ischemia time 7.6 ± 6.1 (n = 775) 7.3 ± 3.5 7.2 ± 3.2 9.3 ± 11.6
    Pancreas recovery to transplant 33.5 ± 20.5 (n = 629) 25.3 ± 18.5 33.3 ± 20.3 41.4 ± 20.0 <0.001
    Death to transplant 52.9 ± 23.6 (n = 796) 43.7 ± 22.1 52.9 ± 23.4 63.1 ± 21.6 <0.001
    Percentage excluding missing cases
    Total (n = 819) Era p-Value
    1999–2002 (n = 220) 2003–2006 (n = 436) 2007–2010 (n = 164)
    Pancreas preservation, % (n) <0.001
    UW only 52.5% 68.2% 49.8% 38.4%
    TLM only 25.3% 19.5% 31.7% 15.9%
    HTK only 8.7% 0.0% 7.3% 23.8%
    Celsior 2.8% 2.3% 1.8% 6.1%
    UW+TLM 4.8% 4.5% 5.0% 4.9%
    Combinations/other 6.0% 5.5% 4.4% 11.0%
    • HTK, histidine-tryptophan-ketoglutarate; TLM, two layer method; UW, University of Wisconsin solution.

    The pancreas digestion enzyme changed significantly during the study period; NB1 collagenase has primarily replaced Liberase HI in the most recent era (p < 0.001, Table 1C). The proportion of in vitro culture more than 6 h has increased significantly over the era (p < 0.001).

    Table 1C. Islet processing
    Total (n = 1017) Era p-Value
    1999–2002 (n = 257) 2003–2006 (n = 472) 2007–2010 (n = 288)
    Digestion enzyme, % (n)
    Collagenase <0.001
    Liberase HI alone 57.8 (n = 588) 91.4 (n = 235) 71.6 (n = 338) 5.2 (n = 15)
    Serva NB1 alone 9.3 (n = 95) 3.6 (n = 17) 27.1 (n = 78)
    ServaGMPColl+ServaNeutProtease 4.7 (n = 48) 16.7 (n = 48)
    CollagenaseP alone 1.5 (n = 15) 0.8 (n = 2) 2.5 (n = 12) 0.3 (n = 1)
    Liberase MTF 0.3 (n = 3) 1 (n = 3)
    Liberase-collagenase blend 4.7 (n = 48) 9.7 (n = 46) 0.7 (n = 2)
    Other/other combination 5.9 (n = 60) 0.4 (n = 1) 7.2 (n = 34) 8.7 (n = 25)
    Not yet reported 15.7 (n = 160) 7.4 (n = 19) 5.3 (n = 25) 40.3 (n = 116)
    Thermolysin (in addition to other) 7.3 (n = 63) 12.5 (n = 56) 4 (n = 7)
    Pulmozyme (in addition to other) 45.4 (n = 391) 14.7 (n = 35) 54.6 (n = 245) 63.4 (n = 111) <0.001
    Purification gradient type, % (n)
    Discontinuous 5.8 (n = 49) 9.8 (n = 21) 5.9 (n = 26) 1.1 (n = 2)
    Continuous 83.8 (n = 704) 77.7 (n = 167) 82.4 (n = 361) 94.1 (n = 176)
    Both 10.2 (n = 86) 12.6 (n = 27) 11.4 (n = 50) 4.8 (n = 9)
    Islet culture (hours)
    Culture time (0-max) 17.7 ± 18.4 (n = 777) 11.9 ± 18.1 18 ± 17.9 24.7 ± 17.5
    Cultured ≥6 h, % (n) 26.5 (n = 269) 14.4 (n = 37) 14.4 (n = 68) 56.9 (n = 164) <0.001
    Percentage excluding missing cases
    Total (n = 857) Era
    1999–2002 (n = 238) 2003–2006 (n = 472) 2007–2010 (n = 288) p-Value
    Digestion enzyme, % (n)
    Collagenase <0.001
    Liberase HI alone 68.6% 98.7% 75.6% 8.7%
    Serva NB1 alone 11.1% 0.0% 3.8% 45.3%
    ServaGMPColl+ServaNeutProtease 5.6% 0.0% 0.0% 27.9%
    CollagenaseP alone 1.8% 0.8% 2.7% 0.6%
    Liberase MTF 0.4% 0.0% 0.0% 1.7%
    Liberase-collagenase blend 5.6% 0.0% 10.3% 1.2%
    Other/other combination 7.0% 0.4% 7.6% 14.5%

    IEQ per islet particle number and IEQ infused were significantly higher during 2007–2010 when compared to 1999–2002 (p < 0.001 and <0.05, respectively, Table 1D) although no significant change in total IEQ at particle count (completion of isolation) was seen. IEQ at count and IEQ infused correlated strongly with each other (r = 0.93, p < 0.0001, Figure S1). Purity of islets and the number of total β cells in 2007–2010 were significantly higher than the previous years (p < 0.01 and <0.05, respectively, Table 1D). The stimulation index and viability declined over the periods (p < 0.001 and <0.01, respectively) but remained above 2.7 and 90.2%, respectively.

    Table 1D. Islet product characteristics
    Total (n = 1017) Era p-Value
    1999–2002 (n = 257) 2003–2006 (n = 472) 2007–2010 (n = 288)
    Islet mass
    Total IEQs (×103) at time of particle count 416 ± 156.9 (n = 773) 417.5 ± 160.6 413.7 ± 159.9 419.2 ± 146.7
    IEQ/islet particle ratio 1.1 ± 0.6 (n = 655) 1.1 ± 0.5 1.1 ± 0.4 1.3 ± 0.8 <0.001
    IEQs infused (×103) 426.5 ± 157.1 (n = 1015) 421.3 ± 155.4 417.8 ± 157.6 445.4 ± 156.8 <0.05
    Packed cell volume infused (mL) 3.9 ± 2.2 (n = 825) 4.1 ± 2.1 3.9 ± 2 3.8 ± 2.8
    Islet quality
    Purity (%) 61.8 ± 18.3 (n = 770) 59 ± 19.1 61.7 ± 18.5 65.1 ± 16.4 <0.01
    Embedded islets (%) 15.7 ± 18.7 (n = 527) 13.6 ± 17.7 16.1 ± 19.5 16.9 ± 18
    Stimulation index 3.3 ± 3.3 (n = 693) 3.8 ± 3.9 3.2 ± 3.2 2.7 ± 2.7 <0.001
    Islet viability (%) 91.2 ± 6.2 (n = 752) 91.4 ± 6.9 91.5 ± 6 90.2 ± 5.9 <0.01
    Total beta cells (×106) 231.1 ± 191.5 (n = 228) 196.2 ± 180 235.6 ± 191.9 318.9 ± 201.4 <0.05
    Total beta cells (×106)/kg recipient 3.6 ± 3.1 (n = 219) 3 ± 2.8 3.8 ± 3.2 4.5 ± 2.8
    Total insulin content of islets (mg) 3.5 ± 2.2 (n = 247) 3.2 ± 2 3.6 ± 2.4 1.7 ± 0.5
    Total endotoxin infused (EU) 22.7 ± 54.9 (n = 667) 29.4 ± 60.7 26.1 ± 60 7.8 ± 26.7
    Total endotoxin (EU)/kg recipient 0.4 ± 0.8 (n = 651) 0.5 ± 1 0.4 ± 0.9 0.1 ± 0.5
    • IEQ, islet equivalent.

    The analysis of donor and isolation characteristics compared by continent (North America vs. European and Australian sites) and by type of transplantation (ITA vs. IAK/SIK) are shown in Tables S1–S5. Briefly, significant differences were seen in the following variables when the data were stratified by continent (p < 0.05): donor body weight, BMI, maximum blood glucose, donor steroid therapy, insulin therapy, transplant-center related pancreas procurement, pancreas preservation, time from death to cross-clamp, cross-clamp to pancreas recovery, death to transplant, pancreas digestion enzyme, islet culture over 6 h, total IEQ at count, IEQ/particle count, IEQ infused, purity, stimulation index, total insulin content of islets, number of islet infusion sequence and the number of total donor(s) per infusion. Analyzed by ITA versus IAK/SIK, the following variables showed significant differences: donor body weight, BMI, cause of death, HbA1c, donor steroid therapy, insulin therapy, time from death to cross-clamp, death to pancreas recovery, pancreas digestion enzyme, purification method, total IEQ at count, IEQ infused, packed cell volume, purity and ratio of embedded islets (Tables S1–S5).

    Table 1E. Islet infusion
    Total (n = 1017) Era p-Value
    1999–2002 (n = 257) 2003–2006 (n = 472) 2007–2010 (n = 288)
    Recipient infusion sequence #, % (n) <0.05
    1st 52.8 (n = 537) 62.3 (n = 160) 46 (n = 217) 55.6 (n = 160)
    2nd 36.4 (n = 370) 31.9 (n = 82) 39.2 (n = 185) 35.8 (n = 103)
    3rd 9.8 (n = 100) 5.4 (n = 14) 14 (n = 66) 6.9 (n = 20)
    4th–6th 1 (n = 10) 0.4 (n = 1) 0.8 (n = 4) 1.6 (n = 5)
    Total donors/infusion, % (n)
    1 90.2 (n = 917) 89.9 (n = 231) 88.6 (n = 418) 93.1 (n = 268)
    2 9.3 (n = 95) 9.3 (n = 24) 11.2 (n = 53) 6.3 (n = 18)
    3 0.5 (n = 5) 0.8 (n = 2) 0.2 (n = 1) 0.7 (n = 2)
    Any positive crossmatch, % (n) 3.1 (n = 20) 0.8 (n = 1) 3.8 (n = 12) 3.7 (n = 7)

    Variables associated with islet product characteristics

    The donor and isolation factors associated with islet product characteristics are shown in Table 2 and Figure 1. Total IEQs at particle count was remarkably associated with donor body weight (Figure 1A), BMI, donor steroid therapy at last admission and blood transfusion (Figure 1B), time from death to cross-clamp, total number of donors per infusion and continent. IEQ/particle count was associated with donor BMI, donor steroid therapy, CIT (Figure 1C), time from death to cross-clamp, pancreas digestion enzymes, era and continent. Packed cell volume correlated negatively with the number of islet infusions sequence (Figure 1D). Islet purity was associated with donor steroid therapy, time from death to cross-clamp, pancreas digestion enzymes (Figure 1E) and continents. The proportion of embedded islets correlated negatively with donor age (Figure 1F). Stimulation index correlated negatively with donor HbA1c (Figure 1G). Viability was negatively associated with Liberase HI (Figure 1H), and associated with pancreas preservation method (Figure 1I). Total β cell number per kilograms of recipient body weight correlated negatively with donor age (Figure 1J), cause of death, steroid and insulin therapy (Figure 1K), purification methods (Figure 1L), islet culture time and era. Total insulin content correlated negatively with donor steroid therapy at the last admission, and correlated positively with total number of donors per infusion and continents. Significant changes were observed in endotoxin content during the analysis period; however, wide variation in data was also noted. This difference observed between sites from different continents could be due to changes in the methodology.

    Table 2. Correlations between donor, procurement, processing characteristics (“factors”; rows) and islet product criteria (columns) in 1017 islet preparations of clinical allogeneic transplantation in CITR, 1999–2010
    Category Factors IEQ at count (1000s) IEQ/particle count ratio Purity (%) Embedded islets (%) Viability (%) Total beta cells/kg recipient Total insulin content (mg)
    Donor characteristics Age (years) −0.183 −0.195
    <0.0001 0.0052
    480 204
    Donor BMI 0.265 0.193
    <0.0001 <0.0001
    733 620
    Trauma death (0 = no, 1 = yes) 0.198
    0.0038
    212
    Donor given steroids during hospital stay (0 = no, 1 = yes) 0.161 0.265 0.172 0.245 −0.257
    0.001 <0.0001 0.0001 0.0613 0.0079
    417 309 487 59 106
    Donor given insulin during hospital stay (0 = no, 1 = yes) 0.255
    0.0005
    184
    Pancreas preservation and recovery Hours from death to cross-clamp 0.157 0.189 0.156
    <0.0001 <0.0001 <0.0001
    653 548 653
    Islet processing Liberase HI −0.18454 0.171
    <0.0001 <0.0001
    639 743
    Serva NB1 0.2338 0.170
    <0.0001 <0.0001
    639 762
    Hours culture time 0.287
    <0.0001
    219
    Islet infusion Total donors (1, 2, 3) 0.182 0.203
    <.0001 0.0013
    773 247
    Era (1 = 1999–2002, 2 = 2003–2006, 3 = 2007–2010) 0.144 0.164
    0.0002 0.0154
    655 219
    Continent (0 = North America, 1 = Europe/Australia) −0.265 −0.199 −0.319 0.189
    <0.0001 <0.0001 <0.0001 0.0029
    773 655 770 247
    • CITR, Collaborative Islet Transplant Registry; IEQ, islet equivalent.
    • Each cell describes, from top to bottom: correlation coefficient, Pr{Rho = 0|H0} number of observations.
    Details are in the caption following the image
    Factors significantly associated with islet product characteristics. (A) Total islet equivalents (IEQs) at particle count versus donor body weight. (B) Total IEQs at particle count versus donor transfusion. (C) IEQ/islet particle ratio versus CIT, cold ischemia time (CIT). (D) Packed cell volume versus infusion number. (E) Purity versus digestion enzyme. (F) Embedded islets versus donor age. (G) Stimulation index versus donor HbA1c. (H) Viability versus digestion enzyme. (I) Viability versus pancreas preservation. (J) Total β cells/kg of recipient weight versus donor age. (K) Total β cells/kg of recipient weight versus donor insulin treatment. (L) Total β cells/kg of recipient weight versus purification type.

    Donor and islet product characteristics associated with clinical outcomes

    Of all the donor characteristics and islet product criteria assessed for their association with primary clinical end points, the only factor significantly associated with any primary outcome was total IEQs infused on achievement of insulin independence over one to several infusions (Figure 2): when at least 600 000 total IEQs were infused, 75–80% of recipients achieved insulin independence compared to 55% achieving insulin independence with <600 000 IEQs infused (hazard ratio = 1.215, p = 0.035), regardless of the number of infusions. (Over the entire CITR period, of the 537 recipients, 31% received one infusion, 50% received two infusions and 17% received three infusions.) No other donor or islet product characteristics were related to this or any other clinical outcome including retention of insulin independence after achievement, or retention of fasting C-peptide >0.3 ng/mL (indicating a functioning graft).

    Details are in the caption following the image
    Effect of total islet equivalents (1000 s) infused, over one to several infusions, on achievement of insulin independence (≥14 days) post–last infusion in clinical islet transplantation (hazard ratio = 1.215, p = 0.035; solid line: <600; dashes: 600 to <1000; dashes-dots: >1000).

    Discussion

    This report represents a comprehensive analysis of clinical-grade islet products from a total of 1017 isolations performed at CITR-participating North American, European and Australian centers. We found a significant increase in IEQs infused in the most recent era and that higher islet mass transplanted was independently associated with the clinical outcome of insulin independence rate. While there are some improvements in islet product criteria for islet transplantation, these criteria have been set so stringently high that it is not possible to compare clinical outcomes across low-, medium- and high-quality islet preparations, thus curtailing our ability to definitively state which specific product criteria influence clinical outcomes. Nonetheless, the improvements noted here in pancreatic islet yield and isolation in this duodecennium have paralleled the notable improvements in clinical outcomes of islet transplantation for the same period 14. Despite the strengths of this study including the largest collection of clinical islets for transplantation ever reported on, limitations are: nonuniformity of islet procurement and preparation methods across several nations in three continents and over a 12-year period; an estimated 80% availability of relevant data in the pertinent geographic regions and calendar time frame; a lack of low- to medium-quality islets for statistical comparison of outcomes.

    Along with increasing IEQs infused by era, donor body weight, BMI and the frequency of steroid and insulin use in the last admission also showed significant gains by era 14. It is expected that higher body weight or BMI correlates with higher islet yield 1, 2, 4, 7, 11-13. This could be due to increased islet mass 1, 11, larger islet size 11 or more efficient digestion of fat-infiltrated pancreata 1. Some studies have found BMI to be a positive predictor of islet viability and insulin secretion 1, 16 but we could not confirm those results here. Surprisingly, we found that as donor BMI rose, so did the infused endotoxin content. This could be a confounder of the data set in which endotoxin values were generally higher at North American sites, where average BMI has increased over time disproportionately to non-North American JDRF sites.

    Reports on age-based yield effects in the literature have been mixed, associating younger donors with higher yields 9, 12, 13, lower yields 5 or no effect 10. We found no direct correlation of donor age to IEQ-based measures. It is likely that the contradictory results of these studies are tied to varying degrees of postpurification recovery, as the correlation between young donor age and a high % of embedded islets, such as we observed here, is well established 2, 9, 12, 17, 18. A second confounding factor may be the variability in definition between “young” and “old.” Another study has instituted a maximum donor age of 50 years, which aligns with a recent observation that the most significant differences arise from segregating donor data above and below 45 years old 10. Given the clear benefits of lower donor age on islet functionality, future research efforts should focus on minimizing the loss of embedded islets during purification.

    Apart from inherent donor characteristics, the data show a trend toward more intensive treatment—that is, more frequent use of steroids and insulin—during the terminal hospital stay, potentially representing recognition of benefits to subsequently donated tissue 7. Although some investigators have suggested that donor fluid resuscitation or blood transfusion may be detrimental to islet yield 6, 13, we found intraoperative transfusions correlated with higher IEQs at particle count. Donor steroid treatment pushed yields higher, which confirms similar results of an earlier study in which steroid use was associated with twice as many successful clinical isolations as nonsteroid treated donors 7. On the other hand, steroid use, in this investigation, was also correlated to reduced insulin and elevated endotoxin in the final islet product. The CITR database provided information on donor insulin treatment, which has not been previously considered as a clinical islet isolation variable. Nevertheless, we found that insulin use was correlated with higher beta cell/kg recipient. This finding is supported by preclinical research suggesting that insulin treatment can promote or maintain islet beta cell mass in rodents 19, 20. The impact of donor vasopressor treatment is unclear and has been correlated to reduced yield 2, elevated yield 7 or no effect 9, 13. We found no significant impact of vasopressors on any of our outcome measures but it is conceivable that a more specific sub-categorization may be necessary to resolve this question.

    Since its introduction more than two decades ago, TLM for packaging and transporting the pancreas to an isolation-capable facility has been vigorously debated. Early experiments on canine pancreata suggested that a bottom layer of oxygenated perfluorochemical could promote cellular respiration in otherwise ischemic tissue and thereby enhance the yield and viability of recovered islets 21, 22. Although clinical experiments initially supported these claims 23, a series of subsequent investigations found no benefit of TLM over simple UW cold storage 24-28 and modern literature reviews have tended to limit the benefits of TLM to long-distance travel 12, 29. The rise and fall of TLM popularity is reflected here in its peak usage between 2004 and 2006 with a significant decline into the modern era. We did not find any full group independent effects, but a small percentage (3%) of cases did see a benefit of TLM on total IEQs, potentially owing to longer transport conditions or to the increased attention and experience demanded of procurement staff to properly implement the technique. Given the cost and difficulty of the procedure and at least one study where TLM packaging was actively detrimental to postculture islet recovery 30, it seems appropriate that the trend toward simple cold storage continues. In that context, UW had historically dominated the choice of cold storage solution for the human pancreas but the less expensive histadine-tryptophan-ketoglutarate (HTK) has seen increasing use in North America. Several studies have reported no difference between UW and HTK on isolation outcome 1, 11, 25, 31 but a few have correlated HTK use to diminished graft survival 32 and reduced yield 13. The CITR data support the finding that HTK solution was associated with reduced islet viability and suggests that a thorough and specific analysis of HTK's influence on clinical outcomes is warranted.

    Changes over time in postisolation processing seem largely focused on islet culture, which has increased dramatically in the last decade. Culturing islets provides a flexible time window for quality control testing, travel accommodations for recipients, recipient pretreatment with immunosuppression to avoid cytokine associated islet injury and the potential for storing or pooling islets for infusion into a compatible recipient 30, 33, 34. The benefits of culture to reducing immunogenicity have been known for some time 33, 35-39 but culturing has also been found to improve islet morphology, increase pellet purity and viability while reducing total tissue volume 30, 34. On the other hand, major islet losses can occur even over short periods 30, 33, 34 as well as increased fragmentation 30, 33, 40, 41 and diminished endocrine function 30, 33, 34. We found that a greater number of culture hours boosted yield and endocrine composition, although the average period here (∼18 h) was significantly shorter than the time periods examined in the studies listed. Whether an infusion of short-term cultured islets results in a better clinical outcome than freshly isolated islets still remains unresolved 33, 34. One promising future direction seems to be in the improvement of culture conditions to better promote islet survival and function. Fraker et al 42 have recently developed a culturing device with a perfluorohydrocarbone-silicone membrane that maintains a more physiological oxygen supply to the islets, minimizing overnight losses and boosting viability.

    The unavailability of Liberase HI for clinical digestion due to FDA recommendation in 2007, has led to major changes in isolation and transplant activity around the world. Numerous studies have examined the differences between Liberase and its most common replacement, Serva NB1, but results have been consistently contradictory. Compared to Liberase, NB1 has been found to improve yield 12, 16, 43, reduce yield 44, encourage viability and endocrine function 43, 44 or diminish it 45, and to shorten digestion time 11, 16 or prolong it 45. There does appear to be general agreement that Liberase results in higher endotoxin content 44-46 and less product purity 44 than NB1, findings that we confirmed in this analysis. We also observed a clear differentiation on yield with Liberase and NB1 negatively and positively correlated to IEQ/particle ratio, respectively. Nevertheless, there is a growing trend towards the use of other digestion enzymes and combinations as various alternatives have become available. The University of Minnesota identified a higher proportion of intact C1 collagenase isoform in Liberase versus NB1, which could account for some of the negative reports on NB1 enzyme 47. It was subsequently shown that VitaCyte-HA collagenase contains the highest available intact C1 and when used in combination with NB1 neutral protease doubled the percentage of successful clinical islet isolations 47, 48.

    Not surprisingly, the use of a discontinuous gradient for isopycnic density purification has almost disappeared, given the technical limitations of this system and its negative impact on yield 49, 50. Accordingly, we correlated a continuous gradient to higher particle count but more interestingly, we found that the use of a discontinuous gradient appeared to increase the beta cell content of the final product. This finding is consistent with another study showing improved islet morphology 4 to suggest that an investigation of the circumstantial benefits of discontinuous gradient purification may be warranted before the technique is altogether abandoned. On the other hand, the necessity of any purification procedure continues to be debated, especially given the difficulty in obtaining critical minimum islet mass from deceased donors and the reported success of nonpurified islets in autologous islet transplant recipients 51.

    Major differences of the present study from previous reports of pancreatic islet isolation include analysis of only clinical-grade islet products, changes over time and data reported from multiple countries on three different continents. Kaddis et al reported their analyses on donor and isolation characteristics of both clinical and research grade islets based on collaboration of multiple islet centers in North America (n of isolations = 1023), revealing that donor age, BMI, CIT, liver/pancreas enzyme, pancreas appearance and preservation method were independently related to islet isolation success 13, 52. In the present study focusing on clinical-grade islet products, the primary end point of islet yield was correlated with donor BMI, steroid therapy at terminal hospital stay, hours between death and cross-clamp, number of donors and continent. These findings confirm that donor and/or selection criteria for clinical isolation are well established.

    Overall, findings in the present report with regard to donor characteristics of age and BMI are in agreement with previously published results. Evidence continues to build that simple cold storage in UW solution may be the best packaging method if applied with due care but the benefits of a postisolation culture period may be outweighed by losses if more physiological culture conditions are not developed and adopted. The debate over Serva NB1 as a substitute for Liberase HI continues but although the present results suggest general benefits from using NB1, the movement toward alternate enzymes and combinations may soon render this argument moot. Finally, purification technique seems to warrant additional study both to improve the recovery of high-functioning but frequently embedded islets from young donors and to evaluate whether some surprising viability benefits of a discontinuous density gradient justify the use of this procedure in specific situations.

    Future directions to improve islet isolation outcomes include modification of pancreas preservation 53, development of new combinations of collagenase or neutral protease 48, optimization of digestion 54, 55 and enhancing purification 56. Attempts to measure islet quantity and quality in objective ways such as digital image analysis, oxygen consumption rate and islet biomarkers should translate into improved clinical efficacy through more precise data analysis 24, 57, 58. The CITR should continue capturing these developments and trends. The ultimate success of any islet isolation and transplantation is, of course, in the benefit to the recipient. The range of measures pertaining to transplant outcome such as euglycemia will be the subject of a forthcoming analysis.

    Acknowledgments

    The CITR is funded by the NIDDK, National Institutes of Health and by a supplemental grant from the JDRF International. Additional data were made available through cooperative agreements with the U.S. United Network for Organ Sharing (UNOS), Alexandria, Virginia, the Administrative and Bioinformatics Coordinating Center of the City of Hope, Duarte, California (1999–2009) and the NIDDK-sponsored CIT (www.citisletstudy.org), coordinated by the University of Iowa Clinical Trials and Data Management Center (2008–present).

    Disclosure

    The authors of this manuscript have no conflicts of interest to disclose as described by the American Journal of Transplantation.

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