Novel Modality of Endoluminal Anastomotic Integrity Assessment with Fluoroangiography After Left-sided Colorectal Resections

Introduction

The current colorectal anastomotic leak rate persists to be relatively high, broadly ranging between 5 and 15% [1]. The pathogenesis of anastomotic colorectal leaks is by all means multifactorial [2, 3]. Although the surgeon may have no power over the modification of tumor and patient-specific variables, surgical technique of anastomotic construction—encompassing lack of tension, adequate vascularity and appropriate application of basic principles of anastomosis creation—is instead directly controllable by the surgeon [1].

Several methods have been described for intraoperative evaluation of the integrity of colorectal anastomoses following left-sided colorectal resections. Technological evolution has allowed to progress from basic mechanical methods to the use of more sophisticated techniques, including oxygen spectroscopy, narrow-band imaging, Doppler flowmetry and fluorescence angiography [3].

The ideal method of anastomotic assessment should allow for a direct visualization of the anastomosis both intra- and extra-luminally, additionally combining the possibility of examining tissue microperfusion at this level.

This study describes a novel endoluminal modality of colorectal anastomotic assessment through the use of a rigid scope introducer also allowing for intraoperative perfusion evaluation by indocyanine green (ICG) fluoroangiography in patients undergoing laparoscopic left-sided colorectal resection.

Materials and methods

The US Food and Drug Administration (FDA)- and European Committee (EC)-approved Disposable Rigid Scope Introducer (DRSI) (Novadaq Technologies Inc., Canada) device is currently commercially available. The DRSI consists of an endoluminal introducer made up of an insertion tube and insufflation port (Fig. 1). The insertion tube length of the DRSI is 255 mm. A seal is positioned within the connection area between the insertion tube and the handle; the seal provides an airtight device valve for insufflation.

Results

The DRSI was successfully used to assess anastomotic integrity after all left-sided colorectal resections performed in 16 consecutive patients (Table 1, 2).

All procedures were performed laparoscopically. No conversions to open surgery, major intra-/postoperative complications or mortality were recorded. Minor postoperative complications (Clavien–Dindo grade I) included one case of ileus, one wound infection and one urinary tract infection. The DRSI allowed to visualize the anastomotic site in all cases with a ‘good’ or ‘excellent’ quality of tissue perfusion by ICG fluoroangiography (Fig. 2). In two cases, the DRSI showed the presence of significant anastomotic bleeding which was successfully controlled by laparoscopic suture placement (Fig. 2) under direct endoluminal control with DRSI. Operative time was increased by an estimated 15 min compared to the same surgical procedures performed without the use of the DRSI. Perioperative outcomes are shown in Table 2.

Discussion

Despite the substantial progress in technologies and refinement of surgical techniques, anastomotic leak rates still continue to complicate an elevated portion of colorectal procedures.

No definitive conclusions can yet be drawn from the current literature regarding the most effective modality of assessing anastomotic integrity [4]. A recent systematic review evaluating different approaches to colorectal anastomotic assessment could not find any superiority of one method over another, although advising to test anastomoses through basic air-leak tests to exclude mere mechanical failure [1].

An endoscopic view of the anastomosis allows for a more precise evaluation of tissue viability, as it has been proved that the mucosal layer has a lower tolerance to ischemia compared to the serosal lining [5]. The DRSI device allows for the concomitant use of a laparoscope and of an introducer combining the possibility of using a high-definition camera with a fluorescence imaging system for tissue perfusion assessment, at the same time conferring the possibility of adequate, albeit minimal, inflation and cleansing of the colorectal lumen for appropriate direct views of the colonic mucosa.

The DRSI demonstrated to have several advantages, including safety, limited costs, ease of use and high reproducibility. Additionally, it contributed to effectively indicate the presence of active anastomotic bleeding or adequate tissue perfusion in all cases. In our cohort of patients, the presence of good quality tissue perfusion visualized endoluminally via the DRSI drove the decision of avoiding loop ileostomies in selected cases. The limits we have experienced during the use of the DRSI include the need for an additional laparoscopic tower, the increase in the overall operative time, the possibility of exploring only up to 25 cm from the anal verge and the impossibility of performing operative maneuvers such as clip or suture placement when necessary (Table 3).

This device holds great potential for prompt intraoperative detection of anastomotic alterations, possibly reducing the risk of anastomotic leaks related to mechanical construction or perfusion issues. The potential advantages of this device warrant larger cohort studies and randomized trials.

Author contributions

All authors contributed to the study designed, manuscript writing, data collection and interpretation of the results. All authors provided critical feedback and helped shape the research, analysis and manuscript. All authors have approved the final version of the article.

Funding

Open access funding provided by Università degli Studi di Roma La Sapienza within the CRUI-CARE Agreement. No funds, grants or other support was received.

Declarations