2.1. Experimental Framework

The study was conducted in the Mampa village (Figure 1).

The village of Mampa belongs to the Kaponda chiefdom, in the Dilanda groupement, Kipushi territory, Haut-Katanga province, DRC. It was founded in 1925 by Mampa of the Lamba tribe. The village, which lies between 11°40 ^′–12°18 ^′ south latitude and 27°18 ^′–27°45 ^′ east longitude, covers an area of 453.1 km² at an altitude of 1240 m, 10 km from the town of Lubumbashi and 83 km from the city of Kasumbalesa, and is inhabited by the Lamba, Tabwa, Tshokwe, Bemba, Sanga, and Luba-Kas peoples. They form around 600–820 households, with an average of 6 people per household. Their main activities are agriculture and the ember trade. Its geographical location is characterized by a tropical climate, with an average annual temperature of 22.4°C and average yearly rainfall of 512.7 mm³. The village has two seasons, with a relatively shorter rainy season from November to April. Characteristic vegetation is of the clear Miombo forest type. The village has no health facilities or schools. All pathologies are treated in the first line of traditional medicine.

2.2. Ethnomedical Data Collection

People living in the Mampa village who had already used medicinal plants to treat illnesses were included in the study, if they had been met in the various households of the Mampa village, that they had agreed to take part in the research and that they had been able to answer the questions at the time of the interviews. The interviews were conducted in Swahili, the language spoken in the village by the entire population, and the local ethnic languages.

2.3. Data Processing and Analysis

The names of the plant species were given by the respondents in the local language during the interviews. The plants were collected in the company of the interviewees. On this occasion, photographs were taken, and herbarium specimens were prepared and deposited at the Kipopo Herbarium, where the plants were formally identified, and given scientific names by the herbarium botanists. The names were updated by comparison with the following databases: Plants of the World Online (https://powo.science.kew.org/), The World Flora Online (http://www.worldfloraonline), or the African Plant Database (https://africanplantdatabase.ch/). Similarly, the geographical distribution of plants is based on POWO data (https://powo.science.kew.org/). Chi-square tests were used to study correlations between qualitative variables. Where some observed numbers were less than 5, the exact Fischer test was used, since the chi-square test is inapplicable in this case. The significance and confidence levels were fixed, respectively, at 5 and 95%. Statistical processing has been performed with GraphPad Prism 10.4.2.

Given the current controversy surrounding ethnobotanical indexes [45], we have used only the terms numbers, relative frequency citation (RFC), usual value (UV), and percentage to assess the results of our investigations quantitatively.

2.4. Ethics Approval, Consent to Participate, Research and Availability of the Study

The Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Lubumbashi (FSPUNILU-DP-BD-062022), reviewed and approved the project proposal and ethical rules. Before the start of data collection, in accordance with the ethical standards of the University of Lubumbashi, all respondents (n = 400) were asked to provide voluntary verbal consent. All participants consented to participate in the study in the presence of a representative of the village head and, in some cases, of the nearest neighbor. Informants were automatically excluded from the study if they did not give their informed verbal consent. All respondents were informed that the data collected would be used for academic purposes only.

The preprint version of this study has been available online on Research Square since the 23rd of September 2024 (https://www.researchsquare.com/article/rs-5116022/v1).

3.1. Knowledge, Attitudes, and Practices of the People of Mampa Village Who Use Plants to Treat Various Pathologies

There are six main reasons why people in the village of Mampa turn to medicinal plants, with the most cited reasons being family tradition (93.8%), distance from hospitals (25.3%), and certainty of cure (23.8%). Mampa people turn to six primary sources for their herbal remedies, dominated by friends (24.5%) and family members (24%). But long before resorting to herbal remedies, the Mampa people, depending on their pathologies, resort to five practices, of which the analysis of signs, symptoms (35%), and tests derived from the ancestral tradition (24%) are reported by nearly three-fifths of those surveyed (Table 1).

Although most of the people in the village of Mampa use plants to treat their illnesses and, in most cases (49%), they are not aware of the risks involved, some of them are aware of certain risks associated with the use of medicinal plants. Of the four risks mentioned, not completing the cure (13%) or aggravating the illness (11.5%) are the two most frequently cited reasons. The Mampa population is aware that the use of medicinal plants does not always produce the expected results and that there have been cases of therapeutic failure. When such cases occur, seven attitudes are observed by the Mampa villagers, of which the two most frequently mentioned are either to do nothing (24%) or to change the plant (22%) (Table 1).

3.2. Knowledge of Medicinal Plants Among the Population of Mampa Village

In total, 38 plants are used by the population of Mampa village to treat 33 pathologies, with Anisophyllea pomifera (46 citations) being the most cited plant and Brachystegia boehmii (5 uses) being the most used plant. The characteristics of these plant relate to their family, morphological, and geographical types, as well as the characteristics of the medicinal recipes derived from their use (Table 2).

3.3. Morphological and Geographical Type

The medicinal plants inventoried in the village of Mampa can be classified into three morphological types, with the tree type representing 76.3% of the total (Figure 2). They can be further divided into 22 geographical types, with plants endemic to Asia representing the majority. However, African endemic plants account for 47.4% of all plants, including 13 from the DRC (Figure 3).

3.4. Family and Local Name

The 38 plants are distributed among 23 genera belonging to 24 families. The most prevalent family is Fabaceae, which accounts for 26.3% of the plants, followed by four families with two plants each: the remaining families are represented by only one plant each (Figure 4). These plants are named in seven local languages of the DRC, of which Tabwa is the most represented with 36 plants. It is followed by Bemba and Lamba with four plants each (Figure 5).

3.5. Used Part and Mode of Preparation

A total of 79 single plant medicinal recipes and two mixed plant medicinal recipes were derived from the 38 plants using seven plant parts. The root was the most utilized plant part, with 21 recipes and 14 plants, followed by the stem bark, which was used in 19 recipes and with 15 plants. The root bark was the third most utilized plant part, with 18 recipes and 10 plants (Figure 6).

The seven organs utilized in a multitude of medicinal preparations are processed via six distinct methods, with decoction being the predominant approach, accounting for 41 recipes and 19 plants (Figure 7). This is followed by maceration, which is employed in 26 recipes and 15 plants.

3.6. Ailments Treated by Plants Inventoried in Our Experimental Framework

The 79 recipes from 38 plants inventoried in this study are used in the treatment of 32 ailments where abdominal pain (12 recipes, 7 plants, 152 citations), diarrhea (7 recipes, 4 plants, 104 citations), hemorrhoids (4 recipes, 3 plants, 96 citations), decreased libido (3 recipes, 3 plants, 76 citations), and cough (3 recipes, 2 plants, 72 citations) are treated by at least 3 medicinal recipes (Figure 8).

3.7. Previous Medicinal Knowledge of the Organs of Inventoried Plants

3.8. Important Medicinal Plants in Mampa

In this study, we consider important local medicinal plants to be those that meet one of three criteria: (i) plants with the highest number of citations, (ii) plants with the highest number of uses, and (iii) plants that have been identified as medicinal for the first time.

The most frequently cited plants are Anisophyllea pomifera and Brachystegia boehmii, with 30 citations each. In contrast, the most frequently utilized plants are Landolphia kirkii (six uses) and Brachystegia boehmii (five uses). This study marks the first time E. delevoyi and Pterocarpus brenanii have been identified as medicinal plants (Figure 9).

3.9. The Sociodemographic Characteristics of the Interviewees

Four hundred resource persons were interviewed as part of this study, with an average age of 48 ± 4 years (ranging from 24 to 72 years). The male-to-female ratio was 0.99. The respondents were either household representatives (93.75%), herbalists (2.50%), or traditional healers (3.75%). The respondents’ mean experience in using medicinal plants was found to be 9 ± 2 years (range: 2–31 years). The participants were engaged in seven primary occupations, with agriculture and livestock breeding representing over 60% of the total. In over 50% of cases, the respondents had only completed elementary school (Table 4).

4.1. Knowledge, Attitudes, and Practices of Traditional Medicine Using Plants in the Mampa Village

The interviews conducted as part of this study revealed that all subjects consulted utilized traditional medicine, predominantly based on plant use. This result is consistent with the findings of a recent study conducted in Lubumbashi, which revealed that 76.4% of the population utilizes traditional medicine, with a notable prevalence of plant-based remedies [21]. This observation has been previously documented in ethnobotanical studies conducted in Haut-Katanga, although these studies were oriented toward specific pathologies [32–34, 35, 36–38, 40, 41].

This study identified family tradition as the primary rationale for the utilization of plants in traditional medicine, a finding that aligns with those of previous research studies [99–102]. The impact of family tradition on the uptake of traditional medicine is a complex phenomenon. These include the following: (i) The practice may be regarded as a cultural heritage that has become an integral part of the family’s identity and heritage [103]. An alternative perspective is that it may be viewed as a means of symbolizing continuity, respect for ancestors, and the link with cultural roots, which may serve to reinforce the feeling of belonging to a community [104]. Thirdly, some families may perceive this practice as a means of achieving greater autonomy and control over their well-being [105]. Fourthly, the practice may have given rise to a positive family tradition passed down through generations. Alternatively, it may serve as an element of family cohesion and reinforcement of the unity of a community, as observed by parents and relatives. In this context, the certainty of cure, identified by this study as a reason for the use of traditional medicine by nearly 24% of the population surveyed (Table 1), may appear to be a secondary justification for the use of traditional medicine based on family practice.

The second most frequently cited rationale for using traditional medicine in this study was the distance to healthcare facilities (Table 1). The lack of physical accessibility to biomedicine has been previously identified as a rationale for using traditional medicine. This is particularly evident in studies conducted in various African countries, including Uganda [106], Rwanda [107], Ethiopia [108, 109], Kenya [101], Nigeria [110], Cameroon [111, 112], Zambia [113], and the DRC, particularly in Haut-Katanga [21]. Although poor accessibility is a common and widely reported reason for the use of traditional medicine in many regions, in the case of the village of Mampa, traditional medicine is the only alternative, given that the village lacks a health structure where modern medicine is practiced. This situation serves to illustrate the role of traditional medicine in primary healthcare in the village of Mampa. These considerations can also be correlated with the sources of knowledge evoked by the population interviewed on the use of traditional medicine, where friends, family, and previous experience figure prominently (Table 1).

It is evident that the diagnostic procedures employed in traditional medicine in the Mampa village are not distinctive when compared to the methods of diagnosis documented in the literature on the diagnosis of pathologies in African traditional medicine [114, 115]. This is particularly the case in the context of the Katanga region [38, 43]. Notably, clinical signs represent the primary foundation for diagnosis in traditional medicine within the Mampa village (91%). The utilization of clinical signs for the diagnosis of illnesses in traditional medicine offers several advantages, including (i) the establishment of a link between traditional medicine and biomedicine, the latter also employing clinical signs to connect manifestations to specific drugs, and (ii) the facilitation of the development of an experimental procedure, particularly in clinical studies, aimed at validating traditional medicine practices [116]. However, this approach has the disadvantage of exposing the patient to the risk of incomplete treatment, of which only a minority of the population surveyed (13%) was aware (Table 1).

Regarding the knowledge of the risks associated with the use of plants in traditional medicine in the village of Mampa, it is notable that several risks identified in this study have been previously documented in other studies [117, 118]. However, some risks have not been previously reported. These include the risk of plant confusion during harvesting, intoxication by the metallic trace elements (TMEs) that specific plant concentrate, and the ecological risk. It is particularly noteworthy that nearly half of the population is unaware of the potential risks associated with using medicinal plants. This could have a detrimental impact on the social stability of the village, particularly given that therapeutic failures may be attributed to witchcraft or mystical practices.

In the event of therapeutic failure when using medicinal plants, two attitudes are predominant in the Mampa village: inaction (24%) and changing the plant (22%). Those who elect to do nothing in the event of therapeutic failure adhere to the belief that there is no alternative to traditional medicine, as they perceive this approach to be infallible. This viewpoint has also been documented in prior research conducted in the region [21, 43]. This indicates the degree of belief in traditional medicine held by this segment of the population, who have likely never encountered a genuine case of therapeutic failure. The remaining majority opinion is to change the plant. This approach is analogous to that of biomedicine, wherein a change in medication is observed in the event of therapeutic failure. This suggests a spectrum of treatment options within traditional medicine in Mampa. Consequently, the mention of two plants in the management of pathology does not necessarily imply that they are used alternatively but that they may also be employed as a palliative measure. It is important to consider this information when collecting ethnobotanical data on traditional Katangan medicine.

4.2. A Variety of Medicinal Plants From the Mampa Village

Traditional medicine in the village of Mampa uses 38 plants. Most of these plants are endemic Asian trees belonging to the Fabaceae family, most of which are named in Tabwa (Table 2).

As evidenced by various ethnobotanical studies conducted in the region [33, 34, 38, 43, 119], the predominance of trees among medicinal plants in Haut-Katanga is a well-documented phenomenon. In contrast to other morphological types, using trees for therapeutic purposes offers several advantages. First, the availability of raw materials is not limited by seasonality, allowing for year-round utilization. Second, using different organs can increase the versatility of the therapeutic supply. Third, the low probability of extinction of highly utilized plants is another advantage.

Many plants inventoried in this study originate from Asia, but only three plants are endemic to the DRC. This differs from various ethnobotanical studies conducted in the Katangese region [36, 39, 120, 121]. The high prevalence of nonendemic medicinal plants in the village of Mampa may indicate a culturally influenced tradition of traditional medicine in this village, potentially shaped by Asian cultural influences. The presence of an Indian agricultural enterprise in the region for nearly two decades supports this hypothesis. However, this hypothesis may be called into question not only by the fact that all the medicinal plants listed are named in local languages, with a predominance of Tabwa, an ethnic group reputed to be among the healing peoples of Katanga, but also by the fact that the acquisition of medicinal knowledge in this village is, in half the cases, the result of a family tradition, dating back to the creation of the village, according to the information gathered during our interviews (Table 1). In the latter case, the plants in question would not have been formally identified as endemic to Katanga.

The preponderance of Fabaceae among the plants inventoried during this study agrees not only with previous ethnomedicinal studies carried out in the area covered by the Miombo open forest [56, 122–126] but also with ethnomedicinal studies carried out in Katanga [36, 38, 39, 43, 127]. This numerical predominance of Fabaceae is globally observed across all plants in sub-Saharan Africa and has been attributed to their ability to capture atmospheric nitrogen, enabling them to grow in any soil type, whether nutrient-rich or nutrient-poor [28, 128].

In the Mampa village, the majority of plants used for therapeutic purposes (94.7%) are wild species. This finding is consistent with the results of previous ethnobotanical studies conducted in the same region [35, 36, 38–40, 127, 129]. The predominant reliance on wild plants as medicinal resources presents both advantages and risks, necessitating a balanced approach. The availability of wild plants without the need for intensive cultivation minimizes ecological impact by reducing pesticide use and preserving local biodiversity. Furthermore, their traditional medicinal applications are often deeply rooted in local knowledge, making them accessible to rural communities, particularly in areas with limited medical infrastructure, such as Mampa village. However, excessive dependence on these uncultivated species poses several risks. The overexploitation of natural resources can have severe consequences, including a decline in population species numbers, which in turn can hinder the ability of these populations to regenerate. This decline can even lead to the extinction of some species. The biochemical composition of wild plants varies significantly due to environmental factors, which complicates the standardization of treatments and raises concerns regarding safety and therapeutic efficacy. Additionally, their occurrence in remote locations may limit access, exacerbating disparities in healthcare availability. Lastly, the absence of environmental control can expose these plants to contaminants such as pollutants and toxins, which may alter their medicinal properties. Therefore, while wild plants play a crucial role in traditional pharmacopeia, their sustainable utilization requires a carefully managed approach that ideally integrates cultivated species to ensure long-term preservation and accessibility.

4.3. Pathologies, Diseases, Signs, or Conditions Treated by Medicinal Plants in the Mampa Village

The results of our surveys indicate that four ailments or conditions are the most prevalent in the Mampa village, with a total of 32 identified. These are gastrointestinal disorders, diarrhea, hemorrhoids, and low libido. Fortunately, the local traditional medicine has plants that can treat these conditions, with seven plants for gastrointestinal disorders, four plants for diarrhea, and three plants for libido and hemorrhoids, respectively (Figure 8).

At the national level, the 10 most prevalent and lethal pathologies in the DRC are malaria, tuberculosis, lower respiratory tract infections, neonatal diseases, diarrheal diseases, strokes, ischemic heart disease, road trauma, hypertensive heart disease, and cirrhosis and other chronic liver diseases [130]. The findings of our surveys indicate that the medicinal knowledge of the Mampa village population may be associated with three of the 10 leading causes of mortality: diarrhea (four plants), malaria (one plant), and lower respiratory tract infections (two plants). These two scenarios, at the local and national levels, illustrate the efficacy of traditional medicine in addressing significant health concerns. Furthermore, these findings underscore the significance of traditional medicine in rural communities, particularly in the context of primary healthcare, as evidenced by numerous prior studies [131–133].

The village of Mampa has been documented to have a variety of ethnomedicinal uses, which have also been observed in other regions of the country and the world.

The ethnobotanical uses reported later in Katanga (DRC) are also observed in the Mampa village. These include the decoction of Anisophyllea pomifera Engl. & Brehmer roots, used in Kipushi (Haut-Katanga, DRC) to treat diabetes [36], and that of Harungana madagascariensis root bark, used in the Kafubu valley (Haut-Katanga) against rheumatism [36, 41]. These same uses are reported in the Mampa village. Similarly, the leaves of Landolphia kirkii are employed in Lubumbashi as a decoction to treat malaria [34, 37]. Additionally, the stem barks of Parinari curatellifolia are utilized in Lubumbashi as a decoction for managing diarrhea [37]. Pterocarpus angolensis stem bark is employed in Lubumbashi as a decoction for treating diabetes [34, 36, 37]. Ultimately, Senegalia polyacantha stem bark is employed in Lubumbashi as a decoction or infusion for managing diarrhea and diabetes, respectively [34, 36]. The uses above were similarly documented in the Mampa village as part of the present study.

Other medicinal applications, documented in both Katanga and other regions, were observed in the Mampa village during our research. These include Crossopteryx febrifuga stem bark, which has been used as a decoction to treat malaria and diarrhea in Kipushi in the DRC [36, 38, 41] and in other countries such as Guinea, Nigeria, and Zimbabwe [64]. Similarly, Brachystegia boehmii leaves are employed in the form of a compress in the Likasi region of Katanga, DRC [39], and as a decoction in Zimbabwe [55] for the treatment of wounds. Furthermore, its ashes are utilized as a therapeutic agent against gonorrhea in the regions as mentioned above [54].

Other ethnopharmacological applications of Mampa have been documented in other global cultures situated outside the DRC. For example, Buddleja davidii roots are used in China against wounds [58] and the Republic of Korea [59]. Butea monosperma roots are used in India against snake venom [61]. In China, Damnacanthus indicus roots are used against cancerous pathologies [66]. The root barks of Juglans nigra are employed in the United States for the management of diarrheal pathologies [74]. The roots of Phoebe lanceolata are utilized in China to manage wounds [77]. The stem bark of Piliostigma reticulatum is used in Senegal [79] and Ivory Coast [78] for the treatment of diarrheal disorders. Based on the findings of our investigation, which are presented in this study, the same ethnopharmacological uses are reported for the same plant in the Mampa village.

The above-cited consensuses, obtained from individuals of disparate backgrounds, demonstrate that there is reason to accord some degree of credibility to the findings of our investigation. Furthermore, they suggest that the results of prospective biological experiments conducted in a laboratory setting are likely to corroborate these uses [38, 39].

4.4. Medicinal Recipes From Mampa Village

The population has identified 32 pathologies in the Mampa villages and developed 81 recipes to treat these conditions, of which two employ two plants and 79 utilize a single plant per recipe. The root is used in 21 recipes and 18 recipes for root bark (see Figure 6), while decoction (see Figure 7) is the most commonly utilized preparation method and plant part.

The preponderance of roots as the part used is also reported in some targeted ethnomedicinal studies previously carried out in the region [32, 34, 39, 41, 119, 134]. In contrast, other targeted ethnomedicinal studies have indicated that the leaf is the most commonly used part [33, 35, 37, 40]. The lack of consensus on which organ is the most widely used in traditional medicine in Katanga may be justified by the diversity of localization of bioactive phytochemical compounds within the plant, which can vary from one pathology to another. The utilization of roots offers certain advantages, including providing primary metabolites, vitamins, and minerals such as carbohydrates, pectin, calcium, and vitamin A [135]. Furthermore, the roots can provide access to secondary metabolite groups, including anthocyanins [136], flavanones and flavanols, lipids [136], and phenylpropanoids and organo-heterocycles [127]. However, reliance on the root exposes the plant to extinction, as it is in high demand. It would, therefore, be advantageous for the residents of Mampa to receive instruction in techniques that facilitate the natural regeneration of plant material, which is frequently utilized for therapeutic purposes.

In local traditional medicine, decoction is the preferred method of preparation, as evidenced by the findings of several studies conducted in Haut-Katanga [32–34, 35, 36–41, 119, 134].

In the context of traditional medicine, the preparation of a medicinal formula via decoction offers several advantages. Firstly, this preparation method is optimal for extracting active compounds that remain stable at high temperatures. Secondly, the process does not require the use of sophisticated or costly apparatus, thereby facilitating its implementation on a vast scale. Thirdly, the method is relatively straightforward and does not necessitate the involvement of a skilled operator, which is advantageous in traditional settings. Fourth, it is a cost-effective method that can be readily implemented in a domestic setting. However, decoctions are not an optimal method for the extraction of heat-sensitive components, as the boiling process can lead to their degradation [40, 137–139]. Nevertheless, decoction should not be the default preparation method in a specific local culture. The optimal preparation method should be determined on an individual basis, considering the specific pathology and the nature of the remedy. Only through rigorous biological experimentation can the rational use of each preparation method be accurately assessed.

4.5. Essential Medicinal Plants Discovered in the Mampa Village

The most frequently cited plants in this study are Anisophyllea pomifera and Brachystegia boehmii, while the most widely used plant is Landolphia kirkii. E. delevoyi and Pterocarpus brenanii represent the plants first reported as medicinal plants. It is thus possible to consider these plants as those which have been singled out by this study in the context of Mampa village.

Anisophyllea pomifera is a tree species endemic to the southwestern part of Africa, including countries such as Tanzania, Malawi, Zambia, Burundi, and the DRC (https://www.worldfloraonline.org/taxon/wfo-0000537325). In Tanzania, Anisophyllea pomifera is utilized as a means of deterring rodent pests that prey on maize and beans, and its fruit is consumed [140, 141], as is the case in Burundi [140]. In the DRC, in the southern Katanga region, the decoction of Anisophyllea pomifera’s leaves, roots, and stem bark is utilized for the treatment of dental caries, malaria, abdominal discomfort, mental health disorders, hypertension, and respiratory conditions, including coughs [34, 35]. Some of these applications, in addition to indications against urinary tract infections and diabetes, were documented in the Mampa village during our various surveys (Table 2). In vitro studies have demonstrated that the plant’s methanolic leaf extract exhibits antimicrobial activity against Streptococcus mutans MTCC 890 (minimum inhibitory concentration (MIC): 31.25 μg/mL) and Lactobacillus acidophilus MTCC 447 (MIC: 62.5 μg/mL) [35]. Additionally, the methanolic root extract demonstrated in vitro antiplasmodial activity against Plasmodium falciparum, with an IC₅₀ of 22.1 μg/mL [50]. Additionally, the root was found to contain saponins, tannins, and terpenoids, while the leaves were also found to contain alkaloids and steroids. However, this plant has the same vernacular name (Fungo in Sanga and Lufunga in Tabwa) as Anisophyllea boehmii Engl., with which it shares certain medicinal uses, notably in the management of diabetes and digestive disorders in Haut-Katanga [36]. This suggests a potential case of confusion in the use of these two plants in traditional Katangese medicine. Subsequent simultaneous pharmacognostic and phytochemical studies on both plants would be beneficial to reach a definitive conclusion.

Brachystegia boehmii Taub is an endemic African tree species that is native to the following countries: The species is found in Angola, Botswana, Burundi, Malawi, Mozambique, the DRC, Tanzania, Zambia, and Zimbabwe (https://powo.science.kew.org). The ethnobotanical uses of this plant vary depending on the country and the organ in question. In Mozambique, the roots are employed in a decoction to assuage agitation in patients [142], whereas the leaves are utilized in a decoction to alleviate abdominal discomfort and the effects of snake venom [143]. In Tanzania, the roots are used in a decoction to treat impotence and wounds [54]. In Zimbabwe, the leaves are employed in a decoction for the treatment of wounds, constipation, lumbago, back pain, and dysmenorrhea [55, 142]. Additionally, macerated stem bark is indicated for the management of sexually transmitted infections [144]. In the DRC, the roots are employed as a macerate to treat wounds and coughs, whereas the stem barks are utilized as a decoction to address dysentery, diarrhea, abdominal discomfort, and gonorrhea [40]. This plant is used globally for the same purposes in the Mampa village (Table 2).

To our knowledge, the only pharmacological evidence reported to date concerns the leaves. Chloroformic leaf extract showed in vitro antibacterial activity against Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis, and Escherichia coli, with a DZI ranging from 12 to 21 mm [55]. The ethanolic extract of the leaves showed only weak anti-inflammatory activity of 48% on COX-1 and antioxidant activity with an IC₅₀ of 46 μg/mL [145]. The primary metabolite metabolomic profile of fresh leaves provides information on the presence of 39 primary metabolites, including seven amino acids (glycine (Gly), alanine (Ala), valine (Val), glutamate (Glu), γ-amino butyric acid (GABA), isoleucine (Ile), and threonine (Thr)), two organic acids (threonate and glycerate), and two sugars (galactose and glycerol) [146].

Landolphia kirkii Dyer ex Hook.f is a tree species endemic to southern and central Africa, occurring in the following countries: The species is found in the following countries: Burundi, Central African Republic, DRC, Kenya, Malawi, Mozambique, South Africa, Somalia, Tanzania, Uganda, Zambia, and Zimbabwe. In some regions, various parts of the plant are employed in multiple applications. The fruit is utilized primarily as food in all countries where the plant is endemic, except the DRC, where its decoction is employed for the treatment of constipation [32] and malaria [147]. In South Africa, the roots are employed as an infusion to alleviate dental discomfort [148], diarrhea, and blood purification [149]. In Kenya [150, 151] and Mozambique [56], the plant is used as a decoction to relieve digestive disorders. In the DRC, the plant is used as an infusion to combat urogenital schistosomiasis and intestinal worms [32]. The leaves are primarily utilized in the DRC as a decoction to treat convulsions [50] and as a maceration to combat malaria [50, 152]. The antimalarial use of the plant, as documented in the village of Mampa, is similarly observed in other regions of the DRC [152] and other parts of the continent [153].

In a previous study, the methanolic extract of the leaves demonstrated antiplasmodial activity against local isolates of Plasmodium falciparum with an IC₅₀ of 9.9 μg/mL [50]. To the best of our knowledge, this represents the only validated in vitro use of the leaves to date. No biological studies have been conducted on the roots and stems. However, the methanolic fruit extract is active in vitro against Salmonella typhimurium ATCC 14028, Streptococcus pyogenes ATCC 21059, and Klebsiella pneumoniae ATCC 13883 with MICs ranging from 6.3 to 24 μg/mL. Nevertheless, it exhibits weak antioxidant activity, with an IC₅₀ value greater than 100 μg/mL [154]. A previous phytochemical screening of the fruit revealed the following characteristics of the plant: The chemical composition of the plant includes hexadecanoic acid, 9-octadecanoic acid, tetradecanoic acid, 9,12-octadecanoic acid, and 9,12,15-octadecatrienoic acid [155]. The ash content is reported as follows: The mean value was 2.9 ± 0.0, and the protein content was 12.7 ± 0.3 mg/kg. The results indicated that the sample contained 2.1% ± 0.20% moisture, 0.9% ± 0.1% ash, and 1.8% ± 0.2% fat. The total phenolic content (TPC) was found to be 1250.33 mg of gallic acid equivalent (GAE) per gram of mollified fruit, while the total flavonoid content (TFC) was determined to be 2019.0 mg of rutin equivalent (RE) per gram of mollified fruit [156].

E. delevoyi De Wild is a tree species indigenous to the DRC, Rwanda, and Burundi. To the best of our knowledge, the only data available in the literature concern phytochemistry. These findings indicate that the hexanic extract of the bark of this plant contains azadirone, 14β,15β-epoxyazadirone, 6α-acetoxyazadirone, 6α-acetoxy-14β,15β-epoxyazadirone, and 6α-acetoxy-14β,15β-epoxyazadiradione. Additionally, the phytochemical profile of the bark includes 4-secotirucalla-4(28),7,24-triene-3,21-dioic acid, delevoyin A (3,4-secotirucalla-4(28),7,24-trien-3-oic acid), delevoyin B (6α-acetoxy kihada lactone), limonoid, 11β-acetoxygedunin, and delevoyin C [157]. In the village of Mampa, Entandrophragma delevoyi is employed to treat malaria. Despite the absence of prior documentation regarding this utilization in other regions, a range of Entandrophragma plants are used in traditional medicine as a malaria treatment. These include Entandrophragma angolense, Entandrophragma candollei, Entandrophragma caudatum, Entandrophragma congolense, Entandrophragma palustre, and Entandrophragma utile [158]. The findings of this study thus allow for the inclusion of E. delevoyi among the various plants of this genus.

Pterocarpus brenanii Barbosa & Torre is a tree species endemic to Malawi, Mozambique, Zambia, and Zimbabwe. Despite a lack of documentation attesting to its presence in the DRC, the proximity of the village of Mampa to Zambia may provide a rationale for its inclusion in the village’s traditional medicinal practices. Furthermore, despite the absence of ethnobotanical studies on this topic in the accessible literature, two medicinal uses in the village of Mampa (malaria and cancer) have been documented in various plant of the Pterocarpus genus [159, 160]. It would be beneficial to conduct further research to determine whether these two pathologies may serve as pharmacotaxonomic markers for the entire Pterocarpus genus.

The two plants, Pterocarpus brenanii and E. delevoyi, are particularly interesting candidates for further ethnopharmacological studies so that their medicinal uses in the Mampa village can be validated. The same can be said of plants Parinari curatellifolia, Pterocarpus angolensis, and Senegalia polyacantha, which seem to have some uses specific to the Mampa village.

4.6. Limitations of the Study

Despite the methodological efforts to address the significant concerns that arise in ethnomedicinal studies, particularly data reliability, community access, plant identification, and logistical challenges, this study does not claim to have exhausted all knowledge related to the interaction between plants and the Mampa village population. The study does not give the cutus on the use of these medicinal plants. The study was limited to documenting their medicinal use, thus paving the way for ethnopharmacological studies that should prove their efficacy and safety, thus validating these uses. At a later stage, it will be necessary to complete our knowledge of the other uses of these plants by these peoples (cultural, artistic, nutritional, etc.). Subsequent studies will also have to examine the therapeutic peculiarities of each family, especially since, in the Mampa village, knowledge is acquired and transmitted within the family. Moreover, given that therapeutic expertise in the Mampa village is transmitted orally, there is a risk of variability related to the memory and will of the informants. The influence of the use of plants in the treatment of diseases can cause damage to biodiversity. Since this study did not address this aspect, future studies should focus on it.