2.1. Collection of Material

The collection of samples was directed to only plants with symptoms describe above (Figures 1(a), 1(b)), from June to July 2017. Eighty young agave plants (40–50 cm tall) with symptoms of yellow streaks, which formed long broad spots when they fused, and mottle or irregular whitish-yellow spots that contrast with the blue-green ash color typical of this agave species were collected from commercial plots in Tequila, Jalisco state (20°51′34″N; 103°50′5″W), the region with the highest production of tequila (DO). In addition, from September 2020 to October 2024, 25 A. tequilana plants used as ornamental plants were collected in Chapultepec National Park, Mexico City (CDMX) (19°24′7″N; 99°13′15″W). All plants had the same symptoms as those described above, consistent with a putative viral infection, that we call generically “Agave yellow streak,” whose causal agent has not been previously described in plants grown under natural conditions in Mexico. Finally, five asymptomatic A. tequilana plants grown from seeds were used as negative controls in the RT-PCR assays.

2.2. HTS of Viruses in Blue Agave Plants

Total RNA was obtained from 0.1 g of agave leaves exhibiting yellow streak symptoms collected in the state of Jalisco and in Chapultepec Park, CDMX with RNATrizol (Thermo Fisher Scientific, USA). We formed three mixed samples composed of tissue from five agave plants each. We sequenced three mixed samples of plants from Jalisco and three mixed samples of plants from Chapultepec Park. RNA integrity and concentration were determined by a microchip Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA), which uses the algorithm RIN (RNA integrity number), based on the degradation and concentration of the corresponding bands of ribosomal RNA 18s and 28s, which are separated by electrophoresis [13]. Libraries were constructed with the TruSeq Stranded mRNA kit (Illumina), and sequencing of RNA messengers was performed at the Unidad Universitaria de Secuenciación Masiva del ADN-UNAM (UUSMD) of the Instituto de Biotecnología (IBT) UNAM, campus Cuernavaca, Morelos, with the platform ILLUMINA Next-seq 500 platform, using paired ends, a library size of 300 pb and a reading size of 75 pb [14]. All the sequences obtained were filtered and cleaned with Trimmomatic Ver. 0.32. The quality of HTS was evaluated by Fastqc Ver. 0.12 with a loss of 1% of the total reads obtained. The reads were mapped onto a database of only viruses obtained from GenBank [15], using the software BBMap Ver. 38.26. The mapped and unmapped reads were assembled de novo using the software Trinity Ver. 2.8.4 [16]. The assembled contigs were analyzed with BLASTx to identify viral genomes [17]. Total of reads were analyzed by comparison with complete virus genomes (mapping) obtained from the GenBank (NCBI) using the software BBMap Ver. 38.26 [18]. Moreover, the reads that were not assigned to known viruses were assembled separately and analyzed by BLASTX to identify unknown viral genomes, permitting the search for genomes of other viruses [17].

2.3. Phylogenetic Analysis and Construction of Dendograms

All dendrograms were constructed with the software MEGA 11 [19] applying the neighbor-joining method, the GTR (general time-reversible) model, and 1500 bootstrap pseudoreplicates The analysis included the complete genomes of Vitivirus isolates Jalisco-1 and Jalisco-2, as well as agave Tepovirus, which was analyzed alongside 48 viral sequences from the Betaflexiviridae family. Additionally, the study incorporated agave potexvirus 1 isolates Jalisco and CDMX, with 54 viral sequences from the Alphaflexiviridae family. Furthermore, the Agave badnavirus A isolate Jal1 was included, containing 25 viral sequences from the Badnavirus genus. For the capsid gene dendrograms, a dendrogram (Geneious Bioinformatics software (https://www.geneious.com) with the Jukes–Cantor method) and GTR model with sequences of Vitivirus and Tepovirus obtained by RT-PCR were used, along with 19 sequences from other viruses. Finally, for the Potexvirus CP gene dendrogram, the sequence obtained by RT-PCR was used along with 12 sequences from other Potexvirus species. The accession numbers of all viral sequences downloaded from GenBank for phylogenetic purposes were also included in the dendrograms.

2.4. RT-PCR of the Agave Virus CP

To confirm the identity of the viruses obtained by HTS, we designed several sets of primers (Table 1) based on the analysis of the sequences of the complete genome of the identified viruses to amplify highly conserved regions of the CP of the viruses detected in the viral genome (Table 1). Total RNA obtained from 0.1 g of leaves from individual agave plants shows yellow streak symptoms with the kit RNATrizol (Thermo Fisher Scientific, USA). Ten plants from Jalisco and 25 plants from Chapultepec Park were used as template for the one-step RT-PCR assay using the SuperScript III One-Step RT-PCR System with Platinum (Invitrogen, Carlsbad, California, USA) with each oligonucleotide. The conditions of the RT-PCR were 50°C for 30 min and 94°C for 5 min of reverse transcription, denaturation at 94°C for 30 s, hybridization at 51.5°C for 1 min, and extension at 72°C for 1 min over 35 cycles and a single 10 min cycle at 72°C. The obtained RT-PCR products were analyzed by electrophoresis in 1.9% agarose gels, and molecular weight (MW) was calculated by comparison with the MW of 1 kb plus (GIBCO BRL) included in the same gel. Electrophoresis was carried out at 100 V/35 min at room temperature. The RT-PCR products obtained were sequenced by the Sanger method [20] and incorporated into the GenBank database to obtain accession numbers and later compare them with the sets of sequences obtained by HTS (NCBI/GenBank). We cloned and sequenced three of the RT-PCR products. The probable new strains of a Vitivirus were cloned using (NEB PCR cloning Kit, BioLabs, New England) and sequenced by the Sanger method. Their sequences were compared with those obtained by HTS and those from Sanger’s CP method. Dendrograms with the cloned and noncloned sequences of the CP were then constructed.

3.1. HTS of Viruses in Blue Agave Plants

3.1.1. Sequence Analysis of Vitivirus

Three samples, each made up of a mix of 5 samples of A. tequilana from Jalisco, and three samples, each composed of 5 samples of agaves from Chapultepec Park, were sequenced by RNA-seq. We obtained 120 million raw reads (20 million per sample) with a size of 75 pb. For the Jalisco plants, we obtained three contigs of 6919–6949 nt of a complete Vitivirus genome. By pairwise comparison of nucleotide sequences, two of them (KY190215; PP216715) showed similarities of 64.5% and another (OR574034) was 64.28% similar to sequences of Agave tequilana vitivirus 1 (MW328756.1) [21] and Agave vitivirus A (MH898468.1), respectively. According to the species demarcation criteria for Vitivirus (Fam: Betaflexiviridae) of ICTV (International Committee on Taxonomy of Virus; https://ictv.global/report_9th/RNApos/Betaflexiviridae) (< 72% nucleotide identity or < 80% amino acid identity between their CP or polymerase genes), the results suggest that one of the contigs obtained from Agave tequilana, designated as Agave yellow streak virus Jalisco-1 (AYSV-Jalisco1), may represent a previously unreported strain of Vitivirus. This strain was found exclusively in commercially cultivated A. tequilana in Jalisco. Nine similar Vitivirus contigs were detected in symptomatic agave plants collected in Mexico City, one of which, a 6925 nt complete genome (OR574033), by pairwise comparisons of nucleotide sequences showed similarities of 79.0%–91.5% with MW328756.1, MH898468.1, and OR574034 Vitivirus (isolated from Jalisco plants), respectively.

The phylogenetic analyses performed with the complete genome sequence of the Betaflexiviridae family and the genomes of the viruses in agaves from Jalisco (KY190215, PQ156977, OR574034) and from Mexico City (OR574033) identified the viruses as Vitiviruses, and Tepovirus was identified only in agaves from Jalisco (OR574037). The AYSV-Jalisco1 (KY190215 and PQ156977) isolated from Jalisco plants clustered on a unique clade. Agave yellow streak virus Jalisco 2 (AYSV-Jalisco2) (OR5740343 from Jalisco and OR574034 from Mexico City) clustered with other agave Vitiviruses, MW328740 and MW328756.1. These results supported the proposal that Agave yellow streak virus Jalisco 1 is a different strain of Agave yellow streak virus Jalisco 2, Agave vitivirus 1, and Agave vitivirus A and that these last three are the same virus (Figure 2).

3.1.3. Sequence Analysis of Potexvirus

We identified complete genomes of a Potexvirus (OR574035 and OR574036) from Mexico City and Jalisco samples, respectively. The pairwise comparisons of nucleotide sequences showed 86% similarity to the sequence MW328740.1 agave potexvirus 1 [21], and according to the species demarcation criteria (< 72% in nucleotides and < 80% in amino acids in CP and RdRp) (Fam: Alphaflexiviridae ICTV; https://ictv.global/report_9th/RNApos/Alphaflexiviridae), they are the same species. Likewise, the phylogenetic study conducted with the complete genome sequence of the viruses from the Alphaflexiviridae family, together with the complete genome sequence of the sequences obtained by HTS of agave potexvirus 1 in Mexico City, MW328740.1 (Figure 3) previously reported in agave RNA-seq data [21], confirmed the identity of agave potexvirus 1 isolated from A. tequilana in Mexico.

3.1.4. Analysis of Partial Sequences of Badnavirus and Other Viruses

Pairwise comparison of a sequence of the partial genome of a Badnavirus (OR574038.1 and PQ032000) from Jalisco samples revealed 90% similarity in nucleotides to the sequence MH898467.1 Agave Badnavirus A available in the GenBank. The species demarcation criterion is > 20% difference in the polymerase gene; therefore, the Badnavirus obtained is the same as the one previously reported. The phylogenetic analysis confirmed its identity with the genus Badnavirus, as it clustered with Kalanchoe Badnavirus and Pineapple Badnavirus clade (Figure 4). We identified other contigs that were related to viruses; these are presented in supporting information (Supporting information Table 1).

The viruses detected and identified in Agave tequilana plants grown in natural commercial conditions in Jalisco state and in agave plants used as ornamentals in Chapultepec National Park indicated the presence of a viral complex associated with symptoms of streaking and yellow mottling, in which the presence in Mexico of seemingly new, previously unreported, strains of Vitivirus and other viruses were outstanding. We named this complex preliminarily Agave yellow streak virus Jalisco_1, the most frequently found in the samples from Jalisco and Chapultepec Park. Moreover, we identified sequences of strains of Potexvirus and Tepovirus (Order: Tymovirales) and a Badnavirus (Family: Caulimoviridae. DNA Reverse Transcribing genome) not previously reported in Mexico. All these viruses should be characterized in greater depth and their distribution in Mexico and in other agave species determined. Even in Mexico, the center of origin, it is unknown whether its numerous species are infected with these or other still unknown viral strains or species.

3.2. RT-PCR of the Agave Virus CP

Using Sanger’s method, we sequenced amplicons of the partial CP gene of AYSV-Jalisco1 (562pb) obtained by RT-PCR from 80% of symptomatic plant samples from Jalisco (PP203279 Agave yellow streak virus-Jalisco-1 consensus sequence) and 60% of symptomatic agave ornamental plant samples from a public garden in Mexico City (PP203280 Agave yellow streak virus-Jalisco-1 consensus sequence), which showed 91% similarity to the genomes KY190215 Agave yellow streak virus-Jalisco-1 isolate Jal2 and PP216715 Agave yellow streak virus-Jalisco-1 isolate Jal1 and 68% similarity to OR574034 Agave yellow streak virus-Jalisco-2 isolate Jal and OR574033 Agave yellow streak virus-Jalisco-2 isolate CDMX obtained with HTS in pairwise comparison of nucleotide sequences. No amplicons were obtained from asymptomatic plants obtained from seed. Three RT-PCR products of the Vitivirus CP from Jalisco were cloned (NEB PCR cloning Kit, BioLabs, New England) and sequenced by the Sanger method and their sequences (PP908501, PP908502, PP908503) compared with those obtained by HTS and with those not cloned, resulting in pairwise comparison similarity of 91% nt and 95% aa to KY190215 and PP216715 and 68%–70% nt and 70%–71% aa similarity to OR574034 and OR574033. Analysis of the whole viral genome of sequences from agave showed a genomic organization that is typical of the members of the Vitivirus genus (Order: Tymovirales) [23] and described its genomic map with the position of the different open-reading frames (ORF) and their noncoding regions (UTR). According to the species demarcation criteria proposed by ICTV [15] for Vitivirus (less than about 72% nt identity or 80% aa identity between their CP or polymerase genes), it could be considered a strain of Vitivirus different from those described so far. This coincides with one of the Vitivirus genomes found in Agave tequilana from Jalisco, Mexico, that we tentatively named Agave yellow streak virus Jalisco-1. RT-PCR products of Tepovirus and Potexvirus were obtained in the expected size and sequenced by Sanger’s method, and dendrograms were constructed. The CP nucleotide sequences obtained by HTS, cloned, and noncloned RT-PCR products were compared in a dendrogram (Geneious Bioinformatics software (https://www.geneious.com) with the Jukes–Cantor model) and grouped according to sequences of the genus Vitivirus available in GenBank [22] (Figure 5). For the AVT, amplicons was obtained in the Jalisco sample (OR589400 and OR589401), which showed a pairwise comparison similarity of 100% in nt and 100% in aa, respectively, with the sequence obtained by HTS (OR574037) (Figure 5). For Agave potexvirus_1, amplicons (OR589404) were obtained in the Jalisco samples (OR589402) and in the Chapultepec samples (OR589403), which showed a 98% nt and 100% aa pairwise comparison similarity, respectively, with the sequence obtained by HTS (OR574036) (Figure 6). However, it was not possible to obtain PCR products for sequencing a Badnavirus and only partial sequences of the genome of this virus were obtained by HTS, and so the complete genome of this Badnavirus found in the agave samples from Jalisco must be determined by methods other than HTS. In summary, mixed infections were present in 100% of the plants tested. The type of cultivation of the agave plant by vegetative propagation could be a reason for the presence of mixed infections, by cloning a plant infected with certain viruses in several plants with the same viruses. Although we have not assessed the economic impact of viral infections in A. tequilana plants, we have observed that those in which these viruses have been identified exhibit seasons where yellow streak symptoms are evident. However, in two to three subsequent seasons, these symptoms disappear. This could suggest that the plant’s immune system is adapting to the viral infection (data not shown).

For most studies in plant virology, it is essential to be able to establish a proper diagnosis, that is, to recognize a disease and identify precisely the causal virus or viruses in multiple viral infections, which are now more commonly detected than before. To characterize the etiology of a new viral disease, it is necessary to identify the virus, reveal its presence in an infected plant, and verify that it is actually the agent responsible for the symptoms following the rules of Koch’s postulate. Highly efficient, quick, and economical diagnostic techniques are also needed to study the epidemiology of a viral disease, to evaluate the efficiency of control methods or to guarantee that plants or seed are virus-free [24]. This involves performing several individual tests. Using bioassays, electron microscopy, ELISA, RT-PCR, and RT-qPCR [25] can be challenging for highly variable viruses, and so it may be difficult to design a universal assay that detects all known and unknown variants. By contrast, HTS is a comprehensive single test that can detect all viruses, including novel variants [26, 27]. To the best of our knowledge, this is the second study in which HTS has been used with Agave plants as a resource for virus identification. Quintanilha-Peixoto et al. [28] identified approximately 25 viral species in A. sisalana plants; of these species, we identified the Closterovirus in our samples (supporting information 1). Previously, in Mexico, an isolate of Tuberose mild mosaic virus (Potyvirus) was detected and characterized in A. attenuata and A. amica, with symptoms of green mosaic and streak, using HTS. This virus was detected only in these two species of agave, but not in A. tequilana [29]. The identification of novel and unique viruses, such as vitiviruses, tepoviruses, and potexviruses, in A. tequilana plants, but not in other agave species, suggests that human handling may be facilitating the transmission of these viruses to A. tequilana from other plants, where they may have subsequently adapted.

This demonstrates the potential of HTS for identifying new viruses and highlights its ability to compare viruses in both wild and cultivated environments.

The greatest advantage of HTS over other diagnostic approaches is that it gives a complete overview of the viral phytosanitary status of a plant. In theory, HTS can detect all viruses in a single assay, and performance is limited only by the completeness of the reference database (s) against which the sequences are compared. Sequence information obtained can also be used to provide insight into virus population structure, ecology, or evolution or to differentiate virus variants that may contribute differently toward disease etiology. HTS has the potential to reduce the time from virus discovery to development of targeted detection assays such as PCR or LAMP and to contribute to the improvement of existing assays, by elucidating sequence variation within virus populations. Another advantage of HTS is that sequence data can be analyzed by multiple end-users or may be re-analyzed as databases are expanded [10].

In this contribution, using HTS, we identified four viruses and two variants not previously identified in Mexico in A. tequilana with symptoms of striping and yellow mottling. The biological tests, ELISA, PCR, or RT-PCR for the known viruses, could not identify the viruses possibly related to the yellow stripe and mosaic symptoms. However, the flexible rod particles observed by TEM would confirm the presence of the viral genera: Vitivirus, Tepovirus, and Potexvirus. Considering the sequences obtained by HTS, specific oligonucleotides were designed for the CP regions for the identified viruses and sequenced, and the obtained products were cloned, thus confirming the identity of the viruses associated with A. tequilana yellow stripe. Mexico is the center of origin and primary diversification of 200 species of Agave, and although in some species symptoms like those caused by viruses have been observed, it is unknown at this time whether these viruses or other unknown viruses are affecting other species. The strategy of using HTS to determine the virome of Agave species would allow us to know the extent of their distribution, the diversity of possible new species or variants of viruses in this genus of plants, the damage they cause, and perhaps their possible relationship with other species of cultivated or even wild plants.