miR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different Stages
Zhenyu Lu
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorJing Wang
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorXu Wang
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorZhiying Li
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorDan Niu
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorMin Wang
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorJinzhu Xiang
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorCorresponding Author
Yongli Yue
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorCorresponding Author
Yajuan Xia
Inner Mongolia Center for Disease Control and Prevention, Hohhot, China
Search for more papers by this authorCorresponding Author
Xueling Li
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorZhenyu Lu
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorJing Wang
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorXu Wang
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorZhiying Li
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorDan Niu
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorMin Wang
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorJinzhu Xiang
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorCorresponding Author
Yongli Yue
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorCorresponding Author
Yajuan Xia
Inner Mongolia Center for Disease Control and Prevention, Hohhot, China
Search for more papers by this authorCorresponding Author
Xueling Li
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China imu.edu.cn
Search for more papers by this authorAbstract
Human embryonic stem cells (hESCs) possess the ability to differentiate into insulin-producing cells (IPCs), which can be used to treat type I diabetes. miR-375 is an essential transcriptional regulator in the development and maturation of the pancreas. In this study, we optimized a protocol to differentiate hESCs into IPCs and successfully obtained IPCs. Then, we performed overexpression and inhibition experiments of miR-375 on cells at different stages of differentiation and performed RNA-seq. The results showed that the expression of miR-375 fluctuated during hESC differentiation and was affected by miR-375 mimics and inhibitors. miR-375 influences global gene expression and the target genes of miR-375. The overexpression of miR-375 can cause changes in multiple signaling pathways during pancreatic development. miR-375 is a major participant in the differentiation of pancreatic β-cells through different target genes at different stages. This study provides new ideas for further investigation of how microRNAs affect cell fate and gene transcription.
Conflicts of Interest
The authors declare that they have no conflicts of interest.
Open Research
Data Availability
The transcriptome sequencing data used to support the findings of this study are available at https://www.ncbi.nlm.nih.gov/bioproject/PRJNA715080.
Supporting Information
| Filename | Description |
|---|---|
| sci6642983-sup-0001-f1.docWord document, 430 KB | Supplementary Materials Supplementary Table 1: primers of RT-PCR. Figure S1: the effects of different concentrations of Wnt3a on cell differentiation and miR-375 expression. Figure S2: the detection of the expression level of pancreatic-related target genes by RT-PCR at different stages after overexpression of miR-375. Figure S3: the effects of overexpression of miR-375 at different stages on the expression level of transcription factors in different signaling pathways. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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