Antimicrobial and Immunomodulatory Activity of Herb Extracts Used in Burn Wound Healing: “San Huang Powder”
Jia-Ru Wu
Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Search for more papers by this authorYu-Chu Lu
Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Search for more papers by this authorSung-Jen Hung
Department of Dermatology, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Search for more papers by this authorJung-Hsing Lin
Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Search for more papers by this authorKai-Chih Chang
Department of Laboratory of Medicine and Biotechnology, Tzu Chi University, Hualien 97004, Taiwan tcu.edu.tw
Search for more papers by this authorJhong-Kuei Chen
Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Search for more papers by this authorWan-Ting Tsai
Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Search for more papers by this authorCorresponding Author
Tsung-Jung Ho
Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
School of Post-Baccalaureate Chinese Medicine, Tzu Chi University, Hualien 97004, Taiwan tcu.edu.tw
Search for more papers by this authorCorresponding Author
Hao-Ping Chen
Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Department of Biochemistry, Tzu Chi University, Hualien 97004, Taiwan tcu.edu.tw
Search for more papers by this authorJia-Ru Wu
Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Search for more papers by this authorYu-Chu Lu
Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Search for more papers by this authorSung-Jen Hung
Department of Dermatology, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Search for more papers by this authorJung-Hsing Lin
Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Search for more papers by this authorKai-Chih Chang
Department of Laboratory of Medicine and Biotechnology, Tzu Chi University, Hualien 97004, Taiwan tcu.edu.tw
Search for more papers by this authorJhong-Kuei Chen
Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Search for more papers by this authorWan-Ting Tsai
Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Search for more papers by this authorCorresponding Author
Tsung-Jung Ho
Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
School of Post-Baccalaureate Chinese Medicine, Tzu Chi University, Hualien 97004, Taiwan tcu.edu.tw
Search for more papers by this authorCorresponding Author
Hao-Ping Chen
Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien 97002, Taiwan tzuchi.com.tw
Department of Biochemistry, Tzu Chi University, Hualien 97004, Taiwan tcu.edu.tw
Search for more papers by this authorAbstract
“San Huang Powder,” a nonsterile milled herb powder, is frequently used to treat burn wounds in traditional Chinese herbal medicine. However, treating a wound with a nonsterile dressing or reagent is not compatible with the current guidelines in modern medicine. Therefore, we investigated the bactericidal and anti-inflammatory activities of four herb extracts used in “San Huang Powder” in vitro. Meanwhile, an in vivo porcine model with superficial second-degree burns was used for the experiments since the size and skin composition of pigs are the closest to that of the human body. The minimal bactericidal concentration (MBC) of the herb extracts was determined. The in vitro assay indicated that Rhubarb and Phellodendron bark extracts decreased the levels of inflammatory cytokines, IL-8, and GM-CSF on LPS-induced HMEC-1 cells. In accordance with this result, the histopathological evaluation results showed that the efficacy of “San Huang Powder” containing both herb materials was much better than the group without Rhubarb. Our results not only provide a basis to understand why “San Huang Powder” has been used to clinically treat wounds without sterilization directly since ancient times but also show the advantages of using multiple herb materials simultaneously on wound sites to prevent infection during treatment. Rhubarb is the recommended ingredient involved in the preparation of “San Huang Powder” to ensure the healing efficacy of burn wounds.
Conflicts of Interest
The authors declare no conflicts of interest.
Open Research
Data Availability
The data obtained in this research can be made available from the corresponding author upon request or from the indicated sources.
Supporting Information
| Filename | Description |
|---|---|
| ecam2900060-sup-0001-f1.pdfPDF document, 263.6 KB | Supplementary Materials Figure S1: representative photomicrographs showing the time course of wound healing on days 0, 7, 14, and 21. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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