MicroRNA-155 Participates in the Expression of LSD1 and Proinflammatory Cytokines in Rheumatoid Synovial Cells
This article is part of Special Issue:
Ziliang Yu,
Hao Liu,
Jianbo Fan,
Feihu Chen,
Wei Liu,
Ziliang Yu
Department of Orthopaedics, Affiliated Hospital 2 of Nantong University, Nantong University, Nantong 226001, China ntu.edu.cn
Search for more papers by this authorHao Liu
School of Clinical Medicine, Nanjing Medical University, Nanjing 211166, China njmu.edu.cn
Search for more papers by this authorJianbo Fan
Department of Orthopaedics, Affiliated Hospital 2 of Nantong University, Nantong University, Nantong 226001, China ntu.edu.cn
Search for more papers by this authorFeihu Chen
Department of Orthopaedics, Affiliated Hospital 2 of Nantong University, Nantong University, Nantong 226001, China ntu.edu.cn
Search for more papers by this authorCorresponding Author
Wei Liu
Department of Orthopaedics, Affiliated Hospital 2 of Nantong University, Nantong University, Nantong 226001, China ntu.edu.cn
Search for more papers by this authorZiliang Yu,
Hao Liu,
Jianbo Fan,
Feihu Chen,
Wei Liu,
Ziliang Yu
Department of Orthopaedics, Affiliated Hospital 2 of Nantong University, Nantong University, Nantong 226001, China ntu.edu.cn
Search for more papers by this authorHao Liu
School of Clinical Medicine, Nanjing Medical University, Nanjing 211166, China njmu.edu.cn
Search for more papers by this authorJianbo Fan
Department of Orthopaedics, Affiliated Hospital 2 of Nantong University, Nantong University, Nantong 226001, China ntu.edu.cn
Search for more papers by this authorFeihu Chen
Department of Orthopaedics, Affiliated Hospital 2 of Nantong University, Nantong University, Nantong 226001, China ntu.edu.cn
Search for more papers by this authorCorresponding Author
Wei Liu
Department of Orthopaedics, Affiliated Hospital 2 of Nantong University, Nantong University, Nantong 226001, China ntu.edu.cn
Search for more papers by this authorAcademic Editor: Eduardo Dalmarco
Abstract
MicroRNA-155 (miRNA-155) is abundant in fibroblast-like synoviocytes (FLS) in rheumatoid arthritis (RA). Lysine-specific demethylase 1 (LSD1) has been found that it can ameliorate the severity of RA. Tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6 are key proinflammatory cytokines implicated in the pathogenesis of RA. In our study, we investigated whether miRNA-155 participates in the expression of LSD1 and proinflammatory cytokines in rheumatoid synovial cells. First of all, flow cytometry and cell counting kit-8 analysis were employed to explore the apoptosis and proliferation of FLS, respectively. Subsequently, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was applied to probe into the level of miRNA-155 in FLS when stimulated by miRNA-155 molecules. Moreover, RT-qPCR was used to explore the relative LSD1 miRNA expression in FLS when stimulated by miRNA-155 molecules, and Western blot and immunofluorescence assay were applied to probe into the expression level of LSD1. Finally, enzyme-linked immunosorbent assay was employed to analyze the secreting level of proinflammatory cytokines in FLS when stimulated by miRNA-155 molecules. RA-FLS showed a higher apoptosis rate than normal FLS. The cell proliferation of both HFLS and MH7A cells was promoted by miRNA-155 upregulation. Meanwhile, the expression of LSD1 and proinflammatory cytokines in the FLS of RA was also changed by miRNA-155 regulation. In conclusion, miRNA-155 participates in the expression of LSD1 and proinflammatory cytokines in rheumatoid synovial cells. These findings imply a potential function and interaction of miRNA-155 and LSD1.
Conflicts of Interest
The authors declare that there is no conflict of interest regarding the publication of this paper.
Open Research
Data Availability
The data used to support the findings of this study are available from the corresponding author upon request.
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