OsSHOC1 and OsPTD1 are essential for crossover formation during rice meiosis
Yun Ren
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Chongqing Key Laboratory of Economic Plant Biotechnology/Institute of Special Plants, Chongqing University of Arts and Sciences, Yongchuan, 402160 China
These authors contributed equally to this work.Search for more papers by this authorDan Chen
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
These authors contributed equally to this work.Search for more papers by this authorWenjie Li
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorDan Zhou
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorTao Luo
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorGuoqiang Yuan
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorJing Zeng
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorYe Cao
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorZhongshan He
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorTing Zou
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorQiming Deng
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorShiquan Wang
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorAiping Zheng
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorJun Zhu
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorYueyang Liang
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorHuainian Liu
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorLingxia Wang
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorCorresponding Author
Ping Li
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, 410125 China
For correspondence (e-mails [email protected]; [email protected]).Search for more papers by this authorCorresponding Author
Shuangcheng Li
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, 410125 China
For correspondence (e-mails [email protected]; [email protected]).Search for more papers by this authorYun Ren
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Chongqing Key Laboratory of Economic Plant Biotechnology/Institute of Special Plants, Chongqing University of Arts and Sciences, Yongchuan, 402160 China
These authors contributed equally to this work.Search for more papers by this authorDan Chen
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
These authors contributed equally to this work.Search for more papers by this authorWenjie Li
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorDan Zhou
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorTao Luo
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorGuoqiang Yuan
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorJing Zeng
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorYe Cao
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorZhongshan He
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorTing Zou
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorQiming Deng
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorShiquan Wang
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorAiping Zheng
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorJun Zhu
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorYueyang Liang
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorHuainian Liu
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorLingxia Wang
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
Search for more papers by this authorCorresponding Author
Ping Li
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, 410125 China
For correspondence (e-mails [email protected]; [email protected]).Search for more papers by this authorCorresponding Author
Shuangcheng Li
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu, 611130 China
Rice Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 611130 Sichuan, China
State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, 410125 China
For correspondence (e-mails [email protected]; [email protected]).Search for more papers by this authorSummary
Meiosis is essential for eukaryotic sexual reproduction and plant fertility, and crossovers (COs) are essential for meiosis and the formation of new allelic combinations in gametes. In this study, we report the isolation of a meiotic gene, OsSHOC1, and the identification of its partner, OsPTD1. Osshoc1 was sterile both in male and female gametophytes, and it showed a striking reduction in the number of meiotic COs, indicating that OsSHOC1 was required for normal CO formation. Further investigations showed that OsSHOC1 physically interacted with OsPTD1 and that the latter was also required for normal CO formation and plant fertility. Additionally, the expression profiles of both genes were consistent with their functions. Our results suggest that OsSHOC1 and OsPTD1 are essential for rice fertility and CO formation, possibly by stabilizing the recombinant intermediates during meiosis.
Supporting Information
| Filename | Description |
|---|---|
| tpj14214-sup-0001-FigS1.tifTIFF image, 1.7 MB | Figure S1. Sequence analysis of CRISPR-Cas9 mediated Osshoc1 and Osptd1 mutants. |
| tpj14214-sup-0002-FigS2.tifTIFF image, 2.2 MB | Figure S2. Putative amino acid sequence alignment of proteins homologous to OsSHOC1. |
| tpj14214-sup-0003-FigS3.tifTIFF image, 2 MB | Figure S3. Putative amino acid sequence alignment of plant proteins homologous to OsPTD1. |
| tpj14214-sup-0004-FigS4.tifTIFF image, 824.1 KB | Figure S4. Negative controls in the BiFC assay for the interaction between OsSHOC1 and OsPTD1 in tobacco epidermal cells. |
| tpj14214-sup-0005-FigS5.tifTIFF image, 925.3 KB | Figure S5. Immunoprecipitation validation of the interaction between OsPTD1 and OsSHOC1. |
| tpj14214-sup-0006-FigS6.tifTIFF image, 427.5 KB | Figure S6. Protein structure and phylogenetic analysis of OsSHOC1/OsPTD1-related proteins. |
| tpj14214-sup-0007-FigS7.tifTIFF image, 409.6 KB | Figure S7. Experimental validation of the antibodies against OsSHOC1 and OsPTD1. |
| tpj14214-sup-0008-TableS1-S7.docxWord document, 44 KB |
Table S1. Cosegregation analysis of the mutation and sterile phenotype (n = 100). Table S2. Segregation analysis of ko910 in the F2 progenies (n = 100). Table S3. Clones obtained by the Y2H library screening. Table S4. Chiasma frequency in different mutants. Table S5. Segregation analysis of ko-Osptd1 in the F2 progenies (n = 100). Table S6. Primer sequences used in this study. Table S7. Accession numbers of all related sequences in this study. |
| tpj14214-sup-0009-Legends.docxWord document, 5.5 MB |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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