Each laser speckle device prototype allows the choice of up to three different laser wavelengths in the range of 400 nm to 800 nm in total. Speckle pattern analysis gives various speckle parameters, for example, speckle contrast, speckle size, speckle modulation or fractal dimension. The developed laser speckle device prototypes were evaluated investigating three skin issues.

Results

We receive reproducible results from the speckle imaging device. For skin ageing, we found significant changes within three age groups. The effect of a methyl nicotinate treatment was clearly visible and quantifiable using a moorFLPI device as well as our speckle imaging device. In terms of basal cell carcinoma diagnosis, we found significant differences between normal and diseased skin, even though the number of samples was limited.

Conclusion

As shown with first application examples, it was possible to demonstrate the potential of the method for skin evaluation in vivo.

REFERENCES

1Briers JD, Webster S. Laser Speckle Contrast Analysis (LASCA): a non-scanning, full-field technique for monitoring capillary blood flow. J. Biomed Opt. 1996; 1(2): 174-179.
10.1117/12.231359
CAS PubMed Google Scholar
2Boas DA, Dunn AK. Laser speckle contrast imaging in biomedical optics. J Biomed Optics. 2010; 15(1):011109.
10.1117/1.3285504
Web of Science® Google Scholar
3Briers D, Duncan DD, Hirst E, et al. Laser speckle contrast imaging: theoretical and practical limitation. J Biomed Optics. 2018; 18(6):066018.
10.1117/1.JBO.18.6.066018
Web of Science® Google Scholar
4Stewart CJ, Gallant-Behm CL, Forrester K, Tulip J, Hart DA, Bray RC. Kinetics of blood flow during healing of excisional full-thickness skin wounds in pigs as monitored by laser speckle perfusion imaging. Skin Res Technol. 2006; 12(4): 247-253.
10.1111/j.0909-752X.2006.00157.x
CAS PubMed Web of Science® Google Scholar
5Fuji H, Asakura T, Shindo Y. Measurements of surface roughness properties by means of laser speckle techniques. Optics Communications. 1976; 16(1): 68-72.
10.1016/0030-4018(76)90052-3
Web of Science® Google Scholar
6Tchvialeva L, Shenkenfelder W, Doronin A, Meglinski I, Markhvida I, Lee TK. In vivo laser speckle measurements of skin roughness: clinical study, in Optics in the Life Sciences, OSA Technical Digest (online), paper JT3A-28). Optical Society of America; 2015. as provided by the OSA Website https://www.osapublishing.org/abstract.cfm?uri=NTM-2015-JT3A.28
10.1364/BODA.2015.JT3A.28
Google Scholar
7Orun AB, Goodyer E, Seker H, Smith G, Uslan V, Chauhan D. Optimized parametric skin modelling for diagnosis of skin abnormalities by combining light back-scatter and laser speckle imaging. Skin Res Technol. 2014; 20(4): 473-485.
10.1111/srt.12142
CAS PubMed Web of Science® Google Scholar
8Lee TK, Tchvialeva L, Zeng H, McLean DI, Lui H. Laser speckle and skin cancer: skin roughness assessment. In Ninth International Conference on Correlation Optics (Vol. 7388, p. 738816). International Society for Optics and Photonics; 2009, December
Google Scholar
9Tchvialeva L, Dhadwal G, Lui H, et al. Polarization speckle imaging as a potential technique for in vivo skin cancer detection. J Biomed Optics. 2012; 18(6):061211.
10.1117/1.JBO.18.6.061211
Web of Science® Google Scholar
10Crowson AN. Basal cell carcinoma: biology, morphology and clinical implications. Mod Pathol. 2006; 19: S127-S147.
10.1038/modpathol.3800512
PubMed Web of Science® Google Scholar
11Altamura D, Menzies SW, Argenziano G, et al. Dermatoscopy of basal cell carcinoma: morphologic variability of global and local features and accuracy of diagnosis. J Am Acad Dermatol. 2010; 62(1): 67-75.
10.1016/j.jaad.2009.05.035
PubMed Web of Science® Google Scholar
12Carr RA, Taibjee SM, Sanders DSA. Basaloid skin tumours: mimics of basal cell carcinoma. Curr Diagn Pathol. 2007; 13(4): 252-272.
10.1016/j.cdip.2007.05.005
Google Scholar
13Hussain AA, Themstrup L, Jemec GBE. Optical coherence tomography in the diagnosis of basal cell carcinoma. Arch Dermatol Res. 2015; 307(1): 1-10.
10.1007/s00403-014-1498-y
PubMed Web of Science® Google Scholar
14Anderson RR, Parrish JA. The optics of human skin. J Invest Dermatol. 1981; 77(1): 13-19.
10.1111/1523-1747.ep12479191
CAS PubMed Web of Science® Google Scholar
15Zieger M, Springer S, Deumer C, et al. Speckle sensors: laser speckle patterns for diagnostics in dermatology. in Clinical and Preclinical Optical Diagnostics II Vol. EB101 of SPIE Proceedings (p. 11075_59). Optical Society of America; 2019. as provided by the OSA webside https://www.osapublishing.org/abstract.cfm?uri=ECBO-2019-11075_59
10.1117/12.2527153
Google Scholar
16Fischer F, Riesenberg R, Speck U, et al. Mobile image sensors for skin diagnosis (DermaSpec), project co-funded by the German ministery for education and research (BMBF); 2015. https://www.photonikforschung.de/media/sensorik-und-analytik/pdf/DermaSpec_Vor-Ort-Analytik
Google Scholar
17Vaz PG, Humeau-Heurtier A, Figueiras E, Correia C, Cardoso J. Laser speckle imaging to monitor microvascular blood flow: a review. IEEE Rev Biomed Eng. 2016; 9: 106-120.
10.1109/RBME.2016.2532598
PubMed Google Scholar
18Benderoth SC, Hainich R. Optical 3D in vivo skin imaging for topographicalquantitative assessment of cosmetic and medical treatments. Int Conf 3D Body Scanning Technol. 2010 S: 42-51.
Google Scholar
19 Moor Instruments GmbH, moorFLPI data sheet (18.09.2019). https://www.moor.co.uk/de-de/products/previous-system/moorflpi/
Google Scholar
20Silva H, Rosado C, Antunes J, Rodrigues LM. Exploring human in vivo microcirculation with methyl nicotinate in different perfusion conditions. Biomed Biopharm Res. 2014; 11(2): 207-2014.
Google Scholar
21Youssef D, El-Ghandoor H, Kandel H, El-Azab J, Hassabel-Elnaby S. Estimation of articular cartilage surface roughness using gray-level co-occurrence matrix of laser speckle image. Materials. 2017; 10: 714.
10.3390/ma10070714
PubMed Web of Science® Google Scholar
22Pacheco MDCL, da Cunha Martins MFP, Zapata AJP, Cherit JD, Gallegos ER. Implementation and analysis of relief patterns of the surface of benign and malignant lesions of the skin by microtopography. Phys Med Biol. 2005; 50(23): 5535.
10.1088/0031-9155/50/23/008
PubMed Web of Science® Google Scholar
23Tchvialeva L, Zeng H, Markhvida I, McLean DI, Lui H, Lee TK. Skin roughness assessment. New Develo Biomed Eng. 2010; 341-358.
Google Scholar
24Nelson SA, Scope A, Rishpon A, et al. Accuracy and confidence in the clinical diagnosis of basal cell cancer using dermoscopy and reflex confocal microscopy. Int J Dermatol. 2016; 55(12): 1351-1356.
10.1111/ijd.13361
PubMed Web of Science® Google Scholar
25Rosendahl C, Tschandl P, Cameron A, Kittler H. Diagnostic accuracy of dermatoscopy for melanocytic and nonmelanocytic pigmented lesions. J Am Acad Dermatol. 2011; 64(6): 1068-1073.
10.1016/j.jaad.2010.03.039
PubMed Web of Science® Google Scholar

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Volume27, Issue4

July 2021

Pages 486-493

Multi-wavelength, handheld laser speckle imaging for skin evaluation

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