A landscape of hairy and twisted: hunting for new trichome mutants in the Saskatoon Arabidopsis T-DNA population
Corresponding Author
A. Taheri
Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, Canada
College of Agriculture, Human and Natural Sciences, Tennessee State University, 3500 John A. Merritt Blvd., Nashville, TN, 37209-1561
Correspondence
A. Taheri & M. Gruber, Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK S7N 0X2, Canada.
E-mails: [email protected] (AT); [email protected] (MG)
Search for more papers by this authorP. Gao
Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, Canada
Plant Biotechnology Institute, National Research Council, Saskatoon, SK, Canada
Search for more papers by this authorM. Yu
Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, Canada
Search for more papers by this authorD. Cui
Agricultural and Environmental Research Services, ICMS Inc, Fort Saskatchewan, AB, Canada
Search for more papers by this authorS. Regan
Department of Biology, Queen's University, Kingston, ON, Canada
Search for more papers by this authorI. Parkin
Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, Canada
Search for more papers by this authorCorresponding Author
M. Gruber
Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, Canada
Correspondence
A. Taheri & M. Gruber, Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK S7N 0X2, Canada.
E-mails: [email protected] (AT); [email protected] (MG)
Search for more papers by this authorCorresponding Author
A. Taheri
Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, Canada
College of Agriculture, Human and Natural Sciences, Tennessee State University, 3500 John A. Merritt Blvd., Nashville, TN, 37209-1561
Correspondence
A. Taheri & M. Gruber, Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK S7N 0X2, Canada.
E-mails: [email protected] (AT); [email protected] (MG)
Search for more papers by this authorP. Gao
Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, Canada
Plant Biotechnology Institute, National Research Council, Saskatoon, SK, Canada
Search for more papers by this authorM. Yu
Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, Canada
Search for more papers by this authorD. Cui
Agricultural and Environmental Research Services, ICMS Inc, Fort Saskatchewan, AB, Canada
Search for more papers by this authorS. Regan
Department of Biology, Queen's University, Kingston, ON, Canada
Search for more papers by this authorI. Parkin
Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, Canada
Search for more papers by this authorCorresponding Author
M. Gruber
Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, SK, Canada
Correspondence
A. Taheri & M. Gruber, Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK S7N 0X2, Canada.
E-mails: [email protected] (AT); [email protected] (MG)
Search for more papers by this authorAbstract
A total of 88 new Arabidopsis lines with trichome variation were recovered by screening 49,200 single-seed descent T3 lines from the SK activation-tagged population and from a new 20,000-line T-DNA insertion population (called pAG). Trichome variant lines were classified into 12 distinct phenotype categories. Single or multiple T-DNA insertion sites were identified for 89% of these mutant lines. Alleles of the well-known trichome genes TRY, GL2 and TTG1 were recovered with atypical phenotype variation not reported previously. Moreover, atypical gene expression profiles were documented for two additional mutants specifying TRY and GL2 disruptions. In remaining mutants, ten lines were disrupted in genes coding for proteins not implicated in trichome development, five were disrupted in hypothetical proteins and 11 were disrupted in proteins with unknown function. The collection represents new opportunities for the plant biology community to define trichome development more precisely and to refine the function of individual trichome genes.
Supporting Information
| Filename | Description |
|---|---|
| plb12230-sup-0001-TableS1.docWord document, 95 KB | Table S1. Primers used for qPCR analysis on select Saskatoon Arabidopsis lines. |
| plb12230-sup-0002-TableS2.docWord document, 199.5 KB | Table S2. Phenotype classification and Gene Loci for 88 Saskatoon Arabidopsis mutants with trichome variation. |
| plb12230-sup-0003-TableS3.xlsxMS Excel, 41.2 KB | Table S3. List of 204 unique trichome gene loci retrieved from the TAIR database and used to screen FST data known for a sub-set of the SK population to find additional trichome phenotypes. |
| plb12230-sup-0004-TableS4.xlsxMS Excel, 36.3 KB | Table S4. List and description of genes 100 kb upstream or downstream of T-DNA inserts in Saskatoon mutant lines P120 and P137. |
| plb12230-sup-0005-FigS1.docWord document, 2.5 MB | Figure S1. Complete set of Saskatoon Arabidopsis trichome mutants classified according to trichome phenotype categories. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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