Identification of circ_0089153/miR-608/EGFR p53 axis in ameloblastoma via MAPK signaling pathway
Jinwen Liu
Department of Periodontics, School and Hospital of Stomatology, Liaoning Provincial Key Laboratory of Oral Diseases, China Medical University, Shenyang, China
Department of Oral Histopathology, School and Hospital of Stomatology, Liaoning Provincial Key Laboratory of Oral Disease, China Medical University, Shenyang, China
Contribution: Data curation, Formal analysis, Investigation, Validation, Visualization, Writing - original draft
Search for more papers by this authorXue Qiao
Department of Oral Histopathology, School and Hospital of Stomatology, Liaoning Provincial Key Laboratory of Oral Disease, China Medical University, Shenyang, China
Central Laboratory Department, School and Hospital of Stomatology, Liaoning Province Key Laboratory of Oral Disease, China Medical University, Shenyang, China
Contribution: Conceptualization, Methodology, Project administration, Resources, Supervision, Writing - review & editing
Search for more papers by this authorJiayi Liu
Department of Oral Histopathology, School and Hospital of Stomatology, Liaoning Provincial Key Laboratory of Oral Disease, China Medical University, Shenyang, China
Contribution: Formal analysis, Investigation, Software, Validation, Visualization, Writing - review & editing
Search for more papers by this authorCorresponding Author
Ming Zhong
Department of Oral Histopathology, School and Hospital of Stomatology, Liaoning Provincial Key Laboratory of Oral Disease, China Medical University, Shenyang, China
Department of Stomatology, Xiang'an Hospital of Xiamen University, Xiamen, China
Correspondence
Ming Zhong, Department of Oral Histopathology, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Disease, Shenyang, Liaoning, China.
Email: [email protected]
Contribution: Conceptualization, Funding acquisition, Methodology, Project administration, Resources
Search for more papers by this authorJinwen Liu
Department of Periodontics, School and Hospital of Stomatology, Liaoning Provincial Key Laboratory of Oral Diseases, China Medical University, Shenyang, China
Department of Oral Histopathology, School and Hospital of Stomatology, Liaoning Provincial Key Laboratory of Oral Disease, China Medical University, Shenyang, China
Contribution: Data curation, Formal analysis, Investigation, Validation, Visualization, Writing - original draft
Search for more papers by this authorXue Qiao
Department of Oral Histopathology, School and Hospital of Stomatology, Liaoning Provincial Key Laboratory of Oral Disease, China Medical University, Shenyang, China
Central Laboratory Department, School and Hospital of Stomatology, Liaoning Province Key Laboratory of Oral Disease, China Medical University, Shenyang, China
Contribution: Conceptualization, Methodology, Project administration, Resources, Supervision, Writing - review & editing
Search for more papers by this authorJiayi Liu
Department of Oral Histopathology, School and Hospital of Stomatology, Liaoning Provincial Key Laboratory of Oral Disease, China Medical University, Shenyang, China
Contribution: Formal analysis, Investigation, Software, Validation, Visualization, Writing - review & editing
Search for more papers by this authorCorresponding Author
Ming Zhong
Department of Oral Histopathology, School and Hospital of Stomatology, Liaoning Provincial Key Laboratory of Oral Disease, China Medical University, Shenyang, China
Department of Stomatology, Xiang'an Hospital of Xiamen University, Xiamen, China
Correspondence
Ming Zhong, Department of Oral Histopathology, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Disease, Shenyang, Liaoning, China.
Email: [email protected]
Contribution: Conceptualization, Funding acquisition, Methodology, Project administration, Resources
Search for more papers by this authorAbstract
Objectives
This study investigated the role of circular RNAs (circRNAs) in the pathogenesis of ameloblastoma (AB), identifying potential novel targets for future targeted therapy.
Materials and Methods
CircRNA and microRNA (miRNA) profiling in AB were built with microarrays. Six novel circRNAs were validated, circ-miRNA networks were delineated. Hsa-miR-608 was filtered over cross-comparison between database screening, miRNA microarray and validated. Circ-miRNA binding sponge was validated via luciferase reporter assay. Downstream mRNAs were screened. Regulation between miRNAs and mRNAs was confirmed in vitro. Gene interaction networks and circRNA–miRNA–mRNA interaction pathway enrichment analyses were established.
Results
Six differentially expressed circRNAs were selected and validated. According to miRNAs and pathways predicted, six correlated miRNAs were selected, hsa-miR-608 was filtered and validated. The hsa_circ_0089153/hsa-miR-608 binding sponge was validated. Downstream gene interaction networks showed that EGFR and p53 had the strongest co-expression. In vitro transfection results confirmed the suppressive function of miR-608 and EGFR p53. Hsa_circ_0089153/hsa-miR-608/EGFR p53 interaction pathway enrichment analysis confirmed functions mainly enriched in MAPK and related signaling pathways regulating AB progression.
Conclusions
Six novel circRNAs were identified. Hsa_circ_0089153/hsa-miR-608 sponging was validated, hsa-miR-608 downregulated EGFR and p53, which might further regulate cell proliferation, differentiation, apoptosis, and cell cycle processes via the MAPK signaling pathway.
CONFLICT OF INTEREST
All authors declare no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
All data generated or analyzed during this study are included in this published article.
Supporting Information
| Filename | Description |
|---|---|
| odi13788-sup-0001-TableS1-S2.docxWord 2007 document , 19.8 KB | Table S1-S2 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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