Volume 26, Issue 8 pp. 1659-1667

ORIGINAL ARTICLE

The identification of autophagy-related genes in the prognosis of oral squamous cell carcinoma

Longbiao Zhu,

Longbiao Zhu

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China

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Donglin Yan,

Donglin Yan

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China

Contribution: Data curation, Methodology, Validation

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Yan Chen,

Yan Chen

Jiangsu Cancer Centre, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China

Contribution: Methodology, Validation

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Sheng Chen,

Sheng Chen

Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China

Contribution: Methodology, Resources

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Ning Chen,

Corresponding Author

Ning Chen

[email protected]

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China

Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China

Correspondence

Jing Han, Jiangsu Cancer Centre, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, 42 Baiziting Rd., Nanjing, China.

Email: [email protected]

Ning Chen, Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, P.R. China.

Email: [email protected]

Contribution: Project administration, Supervision

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Jing Han,

Corresponding Author

Jing Han

[email protected]

orcid.org/0000-0002-6082-4226

Jiangsu Cancer Centre, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China

Correspondence

Jing Han, Jiangsu Cancer Centre, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, 42 Baiziting Rd., Nanjing, China.

Email: [email protected]

Ning Chen, Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, P.R. China.

Email: [email protected]

Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Writing - review & editing

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Longbiao Zhu,

Longbiao Zhu

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China

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Donglin Yan,

Donglin Yan

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China

Contribution: Data curation, Methodology, Validation

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Yan Chen,

Yan Chen

Jiangsu Cancer Centre, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China

Contribution: Methodology, Validation

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Sheng Chen,

Sheng Chen

Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China

Contribution: Methodology, Resources

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Ning Chen,

Corresponding Author

Ning Chen

[email protected]

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China

Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China

Correspondence

Jing Han, Jiangsu Cancer Centre, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, 42 Baiziting Rd., Nanjing, China.

Email: [email protected]

Ning Chen, Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, P.R. China.

Email: [email protected]

Contribution: Project administration, Supervision

Search for more papers by this author

Jing Han,

Corresponding Author

Jing Han

[email protected]

orcid.org/0000-0002-6082-4226

Jiangsu Cancer Centre, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China

Correspondence

Jing Han, Jiangsu Cancer Centre, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, 42 Baiziting Rd., Nanjing, China.

Email: [email protected]

Ning Chen, Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, P.R. China.

Email: [email protected]

Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Writing - review & editing

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First published: 17 June 2020

https://doi.org/10.1111/odi.13492

Citations: 16

Longbiao Zhu and Donglin Yan contributed equally to this work.

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Abstract

Objectives

The aim of this study was to identify prognostic autophagy-related genes and assess the ability of these genes to predict clinical outcomes in oral squamous cell carcinoma (OSCC).

Subjects and methods

The details of the human autophagy-related genes were obtained from the Human Autophagy Database. The Cancer Genome Atlas database was used to obtain the gene expression profiles and clinical data of patients. Prediction of biological functions of differentially expressed genes was performed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Prognosis-related genes were identified by Cox regression analysis, and the coefficient was applied to construct a prognostic risk score model. The median of the risk score was applied to distinguish between high- and low-risk groups. The Gene Expression Omnibus database, qRT-PCR and immunohistochemistry were used to validate the expression of key genes.

Results

KEGG analyses revealed that differentially expressed genes were mainly enriched in autophagy-related pathways and virus infection. BAK1, BID, NKX2-3 and SPHK1 were identified. The risk score model showed that the high-risk score had poorer overall survival (Kaplan-Meier analysis, p = 1.79 × 10^–7). SPHK1 was upregulated in OSCC tissues and cells, and NXK2-3 was downregulated.

Conclusions

Autophagy-related gene expression profiles may be a potential biomarker for OSCC prognosis.

CONFLICT OF INTEREST

The authors declare that they have no conflicts of interest.

Supporting Information

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Citing Literature

Volume26, Issue8

November 2020

Pages 1659-1667

The identification of autophagy-related genes in the prognosis of oral squamous cell carcinoma

Abstract

Objectives

Subjects and methods

Results

Conclusions

CONFLICT OF INTEREST

Supporting Information

REFERENCES

Citing Literature

References

Information

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The identification of autophagy-related genes in the prognosis of oral squamous cell carcinoma

Abstract

Objectives

Subjects and methods

Results

Conclusions

CONFLICT OF INTEREST

Supporting Information

REFERENCES

Citing Literature

References

Related

Information