Gingival advanced glycation end-products in diabetes mellitus-associated chronic periodontitis: an immunohistochemical study
A. Zizzi
Department of Biomedical Sciences and Public Health - Pathological Anatomy and Histopathology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
Antonio Zizzi and Giacomo Tirabassi contributed equally and are both to be considered as first authors.Search for more papers by this authorG. Tirabassi
Department of Clinical and Molecular Sciences - Endocrinology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
Antonio Zizzi and Giacomo Tirabassi contributed equally and are both to be considered as first authors.Search for more papers by this authorS. D. Aspriello
Department of Odontostomatological and Specialized Clinical Sciences - Periodontology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
Search for more papers by this authorM. Piemontese
Department of Odontostomatological and Specialized Clinical Sciences - Periodontology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
Search for more papers by this authorCorresponding Author
C. Rubini
Department of Biomedical Sciences and Public Health - Pathological Anatomy and Histopathology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
Corrado Rubini, MD, Department of Biomedical Sciences and Public Health, Pathological Anatomy and Histopathology, School of Medicine, Polytechnic University of Marche, Via Conca, 71, 60020 Ancona, Italy
Tel: +390715964804
Fax: +390715964809
e-mail: [email protected]
Search for more papers by this authorG. Lucarini
Department of Clinical and Molecular Sciences - Histology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
Search for more papers by this authorA. Zizzi
Department of Biomedical Sciences and Public Health - Pathological Anatomy and Histopathology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
Antonio Zizzi and Giacomo Tirabassi contributed equally and are both to be considered as first authors.Search for more papers by this authorG. Tirabassi
Department of Clinical and Molecular Sciences - Endocrinology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
Antonio Zizzi and Giacomo Tirabassi contributed equally and are both to be considered as first authors.Search for more papers by this authorS. D. Aspriello
Department of Odontostomatological and Specialized Clinical Sciences - Periodontology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
Search for more papers by this authorM. Piemontese
Department of Odontostomatological and Specialized Clinical Sciences - Periodontology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
Search for more papers by this authorCorresponding Author
C. Rubini
Department of Biomedical Sciences and Public Health - Pathological Anatomy and Histopathology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
Corrado Rubini, MD, Department of Biomedical Sciences and Public Health, Pathological Anatomy and Histopathology, School of Medicine, Polytechnic University of Marche, Via Conca, 71, 60020 Ancona, Italy
Tel: +390715964804
Fax: +390715964809
e-mail: [email protected]
Search for more papers by this authorG. Lucarini
Department of Clinical and Molecular Sciences - Histology, School of Medicine, Polytechnic University of Marche, Ancona, Italy
Search for more papers by this authorAbstract
Background and Objective
The accumulation of advanced glycation end-products (AGEs) seems to play an important role in the development of diabetes mellitus (DM)-associated periodontitis; however, some aspects of this issue are still scarcely known, such as the expression of AGEs in type 1 DM-associated periodontitis and the clinical factors able to affect their accumulation. This study aimed to clarify these points by evaluating the expression of AGEs in DM-associated periodontitis.
Material and Methods
Sixteen systemically and periodontally healthy subjects and 48 subjects suffering from generalized, severe, chronic periodontitis (16 with type 1 DM, 16 with type 2 DM and 16 systemically healthy subjects) were studied clinically, periodontally and metabolically. The immunohistochemical expression of AGEs in gingival tissues was also evaluated.
Results
Subjects affected with type 1 DM presented a significantly higher percentage of AGE-positive cells than did subjects affected with type 2 DM, not only in the epithelium, but also in vessels and fibroblasts. A positive and significant correlation was found between gingival expression of AGEs and length of time affected with DM both in type 1 and type 2 DM; glycated hemoglobin, lipid profile, body mass index and age did not correlate significantly with gingival AGEs in any of the classes of subjects studied.
Conclusions
Gingival AGEs are increased in both type 1 and type 2 DM-associated periodontitis; however, the clinical parameter that determines their accumulation, and therefore their degree of influence on the development of DM-associated periodontitis, may be the duration of DM.
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