Gene expression profiles of oral soft tissue-derived fibroblast from healing wounds: correlation with clinical outcome, autophagy activation and fibrotic markers expression
Corresponding Author
Mariana Andrea Rojas
Department of Oral and Maxillofacial Sciences, Section of Periodontics, Sapienza University of Rome, Rome, Italy
Correspondence
Mariana Andrea Rojas, Section of Periodontics, Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 6 Caserta Street, Rome 00161, Italy.
Email: [email protected]
Search for more papers by this authorSimona Ceccarelli
Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorGiulia Gerini
Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorEnrica Vescarelli
Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorLorenzo Marini
Department of Oral and Maxillofacial Sciences, Section of Periodontics, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorCinzia Marchese
Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorAndrea Pilloni
Department of Oral and Maxillofacial Sciences, Section of Periodontics, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorCorresponding Author
Mariana Andrea Rojas
Department of Oral and Maxillofacial Sciences, Section of Periodontics, Sapienza University of Rome, Rome, Italy
Correspondence
Mariana Andrea Rojas, Section of Periodontics, Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 6 Caserta Street, Rome 00161, Italy.
Email: [email protected]
Search for more papers by this authorSimona Ceccarelli
Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorGiulia Gerini
Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorEnrica Vescarelli
Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorLorenzo Marini
Department of Oral and Maxillofacial Sciences, Section of Periodontics, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorCinzia Marchese
Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorAndrea Pilloni
Department of Oral and Maxillofacial Sciences, Section of Periodontics, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorRojas and Ceccarelli are contributed equally.
Funding information
The study was self-funded by the authors and their institution.
Abstract
Aim
Our aim was to evaluate gene expression profiling of fibroblasts from human alveolar mucosa (M), buccal attached gingiva (G) and palatal (P) tissues during early wound healing, correlating it with clinical response.
Materials and Methods
M, G and P biopsies were harvested from six patients at baseline and 24 hr after surgery. Clinical response was evaluated through Early wound Healing Score (EHS). Fibrotic markers expression and autophagy were assessed on fibroblasts isolated from those tissues by Western blot and qRT-PCR. Fibroblasts from two patients were subjected to RT2 profiler array, followed by network analysis of the differentially expressed genes. The expression of key genes was validated with qRT-PCR on all patients.
Results
At 24 hr after surgery, EHS was higher in P and G than in M. In line with our clinical results, no autophagy and myofibroblast differentiation were observed in G and P. We observed significant variations in mRNA expression of key genes: RAC1, SERPINE1 and TIMP1, involved in scar formation; CDH1, ITGA4 and ITGB5, contributing to myofibroblast differentiation; and IL6 and CXCL1, involved in inflammation.
Conclusions
We identified some genes involved in periodontal soft tissue clinical outcome, providing novel insights into the molecular mechanisms of oral repair (ClinicalTrial.gov-NCT04202822).
CONFLICT OF INTEREST
The authors declare that they have no conflict of interests.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
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| jcpe13439-sup-0001-AppendixS1.docxWord document, 416.1 KB | Appendix S1 |
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