Leaf extract of Rhus verniciflua Stokes protects dopaminergic neuronal cells in a rotenone model of Parkinson's disease
Corresponding Author
Seung Kim
Department of Alternative Medicine, Gwangju University
These authors contributed equally to this study.
Sung-Jun Kim, Department of Biotechnology, Chosun University, 375 Seosuk-dong, Dong-gu, Gwang-ju, Republic of Korea 501-759.E-mail: [email protected]Search for more papers by this authorSe-Eun Park
Department of Biotechnology, Chosun University, Gwangju
These authors contributed equally to this study.
Search for more papers by this authorKumar Sapkota
Department of Biotechnology, Chosun University, Gwangju
Central Department of Zoology, Tribhuvan University, Kirtipur, Kathmandu, Nepal
Search for more papers by this authorMyung-Kon Kim
Department of Bio-food Technology, Chonbuk National University, Iksan
Search for more papers by this authorSung-Jun Kim
Department of Biotechnology, Chosun University, Gwangju
Search for more papers by this authorCorresponding Author
Seung Kim
Department of Alternative Medicine, Gwangju University
These authors contributed equally to this study.
Sung-Jun Kim, Department of Biotechnology, Chosun University, 375 Seosuk-dong, Dong-gu, Gwang-ju, Republic of Korea 501-759.E-mail: [email protected]Search for more papers by this authorSe-Eun Park
Department of Biotechnology, Chosun University, Gwangju
These authors contributed equally to this study.
Search for more papers by this authorKumar Sapkota
Department of Biotechnology, Chosun University, Gwangju
Central Department of Zoology, Tribhuvan University, Kirtipur, Kathmandu, Nepal
Search for more papers by this authorMyung-Kon Kim
Department of Bio-food Technology, Chonbuk National University, Iksan
Search for more papers by this authorSung-Jun Kim
Department of Biotechnology, Chosun University, Gwangju
Search for more papers by this authorAbstract
Objectives The present study investigated the neuroprotective effects of Rhus verniciflua Stokes (RVS) leaf extract on rotenone-induced apoptosis in human dopaminergic cells, SH-SY5Y.
Methods Cells were pretreated with RVS extract for 1 h then treated with vehicle or rotenone for 24 h. Cell viability, cell cytotoxicity, cell morphology and nuclear morphology were examined by MTT assay, lactate dehydrogenase release assay, phase contrast microscopy and staining with Hoechast 33342, respectively. Reactive oxygen species were measured by 2′7′-dichlorofluorescein diacetate and fragmented DNA was observed by TUNEL assay. Mitochondrial membrane potential was determined by Rhodamine 123. Pro-apoptotic and anti-apoptotic proteins and tyrosine hydroxylase were analysed by Western blotting.
Key findings Results showed that RVS suppressed rotenone-induced reactive oxygen species generation, cellular injury and apoptotic cell death. RVS also prevented rotenone-mediated changes in Bax/Bcl-2 levels, mitochondrial membrane potential dissipation and Caspase 3 activation. Moreover, RVS pretreatment increased the tyrosine hydroxylase levels in SH-SY5Y cells.
Conclusions These findings demonstrate that RVS protects SH-SY5Y cells against rotenone-induced injury and suggest that RVS may have potential therapeutic value for neurodegenerative disease associated with oxidative stress.
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