Mangifera indica L. extract (Vimang) and mangiferin reduce the airway inflammation and Th2 cytokines in murine model of allergic asthma
Corresponding Author
Dagmar García Rivera
Dagmar García Rivera, Pharmacy PhD, Center for Pharmaceutical Chemistry, 200 and 21 Street, Atabey, Playa, Havana City, Cuba.E-mail: [email protected]Search for more papers by this authorIvones Hernández
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorNelson Merino
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorYilian Luque
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorAlina Álvarez
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorYanet Martín
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorAylin Amador
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorLauro Nuevas
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorRené Delgado
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorCorresponding Author
Dagmar García Rivera
Dagmar García Rivera, Pharmacy PhD, Center for Pharmaceutical Chemistry, 200 and 21 Street, Atabey, Playa, Havana City, Cuba.E-mail: [email protected]Search for more papers by this authorIvones Hernández
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorNelson Merino
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorYilian Luque
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorAlina Álvarez
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorYanet Martín
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorAylin Amador
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorLauro Nuevas
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorRené Delgado
Laboratory of Pharmacology, Department of Biomedical Research, Center for Pharmaceutical Chemistry, Atabey, Playa, Havana City, Cuba
Search for more papers by this authorAbstract
Objectives The aim was to study the effects of Mangifera indica extract and its major component mangiferin on lung inflammation response and Th2 cytokine production using a murine experimental model of allergic asthma.
Methods BALB/c mice were intraperitoneally sensitized with 10 µg of ovoalbumin (OVA) adsorbed on aluminium hydroxide on days 0, 7 and 14. Seven days after the last injection, the mice were challenged with 2% aerosolized OVA inhalation for 30 min beginning on day 21 and continuing until day 24. To evaluate the protective effect, mice were orally treated with M. indica extract (50, 100 or 250 mg/kg) or mangiferin (50 mg/kg) from days 0 to 24. Anti-OVA immunoglobulin E, interleukin (IL)-4 and IL-5 were determined by ELISA and lungs were analysed by histology.
Key findings M. indica extract and mangiferin produced a marked reduction of airway inflammation around vessels and bronchi, inhibition of IL-4 and IL-5 cytokines in bronchoalveolar lavage fluid and lymphocyte culture supernatant, IgE levels and lymphocyte proliferation.
Conclusion This is the first pre-clinical report of the anti-inflammatory properties of M. indica extract and mangiferin in experimental asthma and it could be an important part of pre-clinical requirement necessary for its use to complement the treatment of this complex disease.
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