Inflammasome activation by UVB irradiation in human corneal epithelial cells
Abstract
Purpose
Cornea is the outermost layer of the eye and mainly responsible for the absorption of ultraviolet B (UVB) radiation. An acute painful inflammatory damage caused by UVB radiation on the corneal epithelium is known as photokeratitis, snow blindness, or welder's burn. We have previously shown that UVB induces inflammation by increasing the production of cytokines IL-6 and IL-8 in human corneal epithelial (HCE) cells and now, we have continued this study by exploring the effects of UVB on the inflammasome signaling.
Methods
In vitro-cultured HCE cells were exposed to UVB radiation after priming with TNF-α. Thereafter, inflammasome components and cytokines were determined using the ELISA, western blot, and activity assays.
Results
Our results suggest that UVB is capable of activating inflammasome signaling in HCE cells.
Conclusions
In addition to traditional pathways, UVB induces the activation of more recently found pro-inflammatory signaling system in the human corneal epithelium.
