cis-Urocanic acid prevents inflammation and cell death in UVB-treated ARPE-19 cells
Abstract
Purpose
cis-Urocanic acid (cis-UCA) is an endogenous ultraviolet (UV) absorbing chromophore that is mainly produced in the upper layers of epidermis. The aim of our study was to investigate the cytoprotective capacity of cis-UCA in UVB-irradiated ARPE-19 cells.
Methods
ARPE-19 cells were pretreated with IL-1α ± and cis-UCA and then exposed to UVB radiation. Secretion of IL-1β and -18 was measured using the ELISA method. Moreover, the cells were observed under an inverted microscope and cell viability was measured by lactate dehydrogenase (LDH) release assay. The proper cis-UCA concentration was evaluated comprehensively beforehand using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide), neutral red, and LDH tests.
Results
Our results show that UVB after IL-1α ± priming activates IL-1β and -18 secretion in ARPE-19 cells and cis-UCA clearly alleviates that. cis-UCA also improves cellular viability.
Conclusions
cis-UCA shows anti-inflammatory and cytoprotective properties in ARPE-19 cells.
