Prevention of herpes simplex stromal keratitis by a glycoprotein B-specific monoclonal
Summary
The increasing incidence of acyclovir (ACV) and multidrug-resistant strains in patients with Herpetic Stromal Keratitis (HSK) is a major health problem and often results in blindness. In the study we examined the effectivity of mAb 2c in preventing experimental HSK in BALB/c mice. Mice were infected with HSV-1 (KOS) and subsequently either systemically or topically treated with mAb 2c. Systemic treatment was performed by intravenous administration of mAb 2c 24 h prior to infection (pre-exposure prophylaxis) or 24, 40, and 56 hours after infection (post-exposure immunotherapy). For topical treatment antibody-containing eye drops or PBS as control was administered (5 times per day). Systemic antibody treatment markedly reduced viral loads and completely protected mice from developing HSK. The administration of antibody prior or post infection was equally effective. Topical treatment had no improving effect on the severity of HSK. In conclusion, our data demonstrate that mAb 2c proved to be effective for the treatment of corneal HSV-infections and for prevention of HSK and blindness. Moreover, the humanized counterpart (mAb hu2c) was equally potent in protecting mice from HSV-induced HSK when compared to the parental mouse antibody.
