Three Putative Photosensory Light, Oxygen or Voltage (LOV) Domains with Distinct Biochemical Properties from the Filamentous Cyanobacterium Anabaena sp. PCC 7120
Rei Narikawa
Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153–8902, Japan
Search for more papers by this authorKazunori Zikihara
Radiation Biology Center, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606–8501, Japan
Search for more papers by this authorKoji Okajima
The Research Institute for Advanced Science and Technology, Osaka Prefecture University, Gakuen-cho, Sakai, Osaka 599–8570, Japan
Search for more papers by this authorYuriko Ochiai
Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153–8902, Japan
Search for more papers by this authorMitsunorl Katayama
Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153–8902, Japan
Search for more papers by this authorYoshinori Shichida
Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606–8502, Japan
Search for more papers by this authorSatoru Tokutomi
The Research Institute for Advanced Science and Technology, Osaka Prefecture University, Gakuen-cho, Sakai, Osaka 599–8570, Japan
Search for more papers by this authorCorresponding Author
Masahlko Ikeuchi
Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153–8902, Japan
*[email protected] (Masahiko Ikeuchi)Search for more papers by this authorRei Narikawa
Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153–8902, Japan
Search for more papers by this authorKazunori Zikihara
Radiation Biology Center, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606–8501, Japan
Search for more papers by this authorKoji Okajima
The Research Institute for Advanced Science and Technology, Osaka Prefecture University, Gakuen-cho, Sakai, Osaka 599–8570, Japan
Search for more papers by this authorYuriko Ochiai
Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153–8902, Japan
Search for more papers by this authorMitsunorl Katayama
Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153–8902, Japan
Search for more papers by this authorYoshinori Shichida
Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606–8502, Japan
Search for more papers by this authorSatoru Tokutomi
The Research Institute for Advanced Science and Technology, Osaka Prefecture University, Gakuen-cho, Sakai, Osaka 599–8570, Japan
Search for more papers by this authorCorresponding Author
Masahlko Ikeuchi
Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Komaba, Meguro, Tokyo 153–8902, Japan
*[email protected] (Masahiko Ikeuchi)Search for more papers by this authorAbstract
Light, oxygen or voltage (LOV) domains function as blue-light sensors in the phototropin family of photoreceptors found in plants, algae and bacteria. We detected putative LOV domains (Alr3170-LOV, A112875-LOV and A1r1229-LOV) in the genome of a filamentous cyanobacterium, Anabaena sp. PCC 7120. These cyanobacterial LOV domains are closely clustered with the known LOV domains. Alr3170-LOV and A112875-LOV carry the conserved cysteine residue unique to the photoactive LOV, whereas A1r1229-LOV does not. We expressed these three LOV domains in Escherichia coli and purified them. In fact, Alr3170-LOV and A112875-LOV that are conserved in Nostoc punctiforme, a related species, bound flavin mononuclcotide and showed spectral changes unique to known LOV domains on illumination with blue light. A1r3170-LOV was completely photoreduced and dark reversion was slow, whereas A112875-LOV was slowly photoreduced and dark reversion was rapid. For comparison, AvA112875-LOV in a closely related A. variabilis was also studied as a homolog of A112875-LOV. Finally, we observed that A1r1229-LOV that is not conserved in N. punctiforme showed no flavin binding.
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