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Selective Photocleavage of DNA and RNA by Anthraquinone Derivatives: Targeting the Single-Strand Region of Hairpin Structures
Maria Fernandez-Saiz,
Paul T. Henderson,
W. David Wilson,
Gary B. Schuster,
Maria Fernandez-Saiz
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA
Search for more papers by this authorPaul T. Henderson
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA
Search for more papers by this authorW. David Wilson
Department of Chemistry, Georgia State University, Atlanta, GA, USA
Search for more papers by this authorCorresponding Author
Gary B. Schuster
Department of Chemistry, Georgia State University, Atlanta, GA, USA
*School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332–0400, USA. Fax: 804–894–7539Search for more papers by this authorMaria Fernandez-Saiz,
Paul T. Henderson,
W. David Wilson,
Gary B. Schuster,
Maria Fernandez-Saiz
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA
Search for more papers by this authorPaul T. Henderson
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA
Search for more papers by this authorW. David Wilson
Department of Chemistry, Georgia State University, Atlanta, GA, USA
Search for more papers by this authorCorresponding Author
Gary B. Schuster
Department of Chemistry, Georgia State University, Atlanta, GA, USA
*School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332–0400, USA. Fax: 804–894–7539Search for more papers by this authorAbstract
A tetracationic anthraquinone derivative (27AQS2) binds to hairpin DNA and RNA. Ultraviolet irradiation of the bound quinone causes cleavage in the loop region of both oligonucleotides and at guanines in the stem region of the DNA hairpin. The absence of observable strand cleavage at guanines in the RNA hairpin suggests that either aniline treatment does not cause cleavage at damaged guanines in RNA or that radical cation migration does not occur readily in RNA duplexes. The ability to target the single-stranded regions of DNA and RNA structures is an important property of this photonuclease.
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