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MULTIPLE MODES OF PHOTODYNAMIC ACTION BY CERCOSPORIN
Philip E. Hartman,
Wendy J. Dixon,
Thomas A. Dahl,
Margaret E. Daub,
Corresponding Author
Philip E. Hartman
Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
*To whom correspondence should be addressed.Search for more papers by this authorWendy J. Dixon
Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
Search for more papers by this authorThomas A. Dahl
Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
Search for more papers by this authorMargaret E. Daub
Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695, USA
Search for more papers by this authorPhilip E. Hartman,
Wendy J. Dixon,
Thomas A. Dahl,
Margaret E. Daub,
Corresponding Author
Philip E. Hartman
Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
*To whom correspondence should be addressed.Search for more papers by this authorWendy J. Dixon
Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
Search for more papers by this authorThomas A. Dahl
Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
Search for more papers by this authorMargaret E. Daub
Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695, USA
Search for more papers by this authorAbstract
Abstract— Cercosporin, one of a number of 4,9-dihydroxyperylene-3,10-quinones synthesized by plant pathogenic fungi, abundantly produces singlet oxygen when illuminated in solution. Singlet oxygen production is substantially decreased and superoxide production is greatly enhanced in the presence of the reducing agents ergothioneine (2-thiol-L-histidine betaine) or urate. Both ergothioneine and urate enhance superoxide production at a rate approximately 25-fold that elicited by an equimolar amount of methionine, the agent that is traditionally used in such assays. Such ‘switching’ in production of different active oxygen species under different environmental conditions may be of significance in biological processes, in this case host cell killing by the plant pathogen.
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