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Fetal Neural Grafts and Repair of the Injured Spinal Cord
Douglas K. Anderson,
Dena R. Howland,
Paul J. Reier,
Corresponding Author
Douglas K. Anderson
Department of Neuroscience
Department of Neurological Surgery University of Florida College of Medicine, JHMHC, Gainesville, Florida, USA
Depatment of Neuroscience, University of Florida Brain Institute, Gainesville, Florida, USA
Gainesville Veterans Affairs Medical Center, Gainesville, Florida, USA
Corresponding author: Dr. Douglas K. Anderson, Department of Neuroscience, P.O. Box 100244, University of Florida College of Medicine, JHMHC, Gainesville, Florida 32610–0244, USA Tel. +1 (904) 392–6641; Fax +1 (904) 846–0250Search for more papers by this authorDena R. Howland
Department of Neuroscience
Depatment of Neuroscience, University of Florida Brain Institute, Gainesville, Florida, USA
Search for more papers by this authorPaul J. Reier
Department of Neuroscience
Department of Neurological Surgery University of Florida College of Medicine, JHMHC, Gainesville, Florida, USA
Depatment of Neuroscience, University of Florida Brain Institute, Gainesville, Florida, USA
Search for more papers by this authorDouglas K. Anderson,
Dena R. Howland,
Paul J. Reier,
Corresponding Author
Douglas K. Anderson
Department of Neuroscience
Department of Neurological Surgery University of Florida College of Medicine, JHMHC, Gainesville, Florida, USA
Depatment of Neuroscience, University of Florida Brain Institute, Gainesville, Florida, USA
Gainesville Veterans Affairs Medical Center, Gainesville, Florida, USA
Corresponding author: Dr. Douglas K. Anderson, Department of Neuroscience, P.O. Box 100244, University of Florida College of Medicine, JHMHC, Gainesville, Florida 32610–0244, USA Tel. +1 (904) 392–6641; Fax +1 (904) 846–0250Search for more papers by this authorDena R. Howland
Department of Neuroscience
Depatment of Neuroscience, University of Florida Brain Institute, Gainesville, Florida, USA
Search for more papers by this authorPaul J. Reier
Department of Neuroscience
Department of Neurological Surgery University of Florida College of Medicine, JHMHC, Gainesville, Florida, USA
Depatment of Neuroscience, University of Florida Brain Institute, Gainesville, Florida, USA
Search for more papers by this authorAbstract
Solid or suspension grafts of fetal spinal cord (FSC), caudal brainstem (FBSt), neocortex (FNCx) or a combination of either FSC/FNCx or FSC/FBSt were placed into cavities produced by static loading (i.e., compression) of the spinal cord of adult cats two to 30 weeks after injury. Extensively vascularized, viable graft tissue was found in all animals with the exception of two cats which showed active rejection of their transplants. Surviving grafts showed many immature characteristics 6–9 weeks after transplantation. However, by 20–30 weeks FSC and FBSt grafts were more mature. Grafts integrated with the host gray and white matter and neuritic processes from both host and graft were seen crossing the host-graft interface. Host calcitonin gene related peptide (CGRP)-like immunoreactive axons could be traced into FSC and FBSt grafts. A more restricted ingrowth of host serotonin (5-HT)-like immunoreactive fibers was seen in FSC grafts. Our results suggest that the capacity of homotypic transplants to promote recovery of function is greater than heterotypic transplants. Additionally, it appears that the functional capacity of the graft depends upon graft survival, the time interval between injury and transplantation, and whether or not the lesion cavity was debrided prior to grafting.
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