The spatiotemporal expression changes of 16 epididymis-specific genes induced by testosterone, heat, and combination treatment in cynomolgus monkey
Xiangqi Li
College of Life Science, Sichuan University, Chengdu 610064, China
Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
These authors contributed equally to this work
Search for more papers by this authorQiang Liu
Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
These authors contributed equally to this work
Search for more papers by this authorShigui Liu
College of Life Science, Sichuan University, Chengdu 610064, China
Search for more papers by this authorXuesen Zhang
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Search for more papers by this authorYixun Liu
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Search for more papers by this authorCorresponding Author
Yonglian Zhang
Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
*Corresponding author: Tel/Fax, 86-21-54921163; E-mail, [email protected]Search for more papers by this authorXiangqi Li
College of Life Science, Sichuan University, Chengdu 610064, China
Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
These authors contributed equally to this work
Search for more papers by this authorQiang Liu
Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
These authors contributed equally to this work
Search for more papers by this authorShigui Liu
College of Life Science, Sichuan University, Chengdu 610064, China
Search for more papers by this authorXuesen Zhang
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Search for more papers by this authorYixun Liu
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Search for more papers by this authorCorresponding Author
Yonglian Zhang
Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
*Corresponding author: Tel/Fax, 86-21-54921163; E-mail, [email protected]Search for more papers by this authorThis study was supported by the grants from the National Natural Science Foundation of China (30230190 and 30570684), the National Basic Science Research and Development Project (2006CB504002 and 2006CB944002) and the Chinese Academy of Sciences Knowledge Innovation Program (No. KSCX1-YW-R-54)
Abstract
The experimental infertility model of treatments involving testicular warming, testosterone implant, and a combination of the two was developed to confirm a synergistic action induced by the combination treatment on germ cell apoptosis in cynomolgus monkey testis. Using this model, the spatio-temporal expression changes of 16 reported or novel genes in epididymis were investigated to examine the treatment's effect on epididymal genes. It was demonstrated that these region-specific genes, some of which were not regionally fixed, changed greatly with these treatments. The expression levels of these epididymal genes fluctuated, and the expression of most of the genes returned to nearly normal level at the end of treatments. Moreover, the expression changes resulting from the combination treatment were not more significant than those resulting from the single treatment. This suggests that the combination treatment has an antagonistic action on the expression of epididymal genes and that its effect is not as adverse on epididymis as that of the two single treatments.
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