Zinc finger protein 278, a potential oncogene in human colorectal cancer

Xiaoqing Tian

Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China

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Danfeng Sun,

Danfeng Sun

Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China

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Yanjie Zhang,

Yanjie Zhang

Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China

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Shuliang Zhao,

Shuliang Zhao

Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China

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Hua Xiong,

Hua Xiong

Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China

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Jingyuan Fang,

Corresponding Author

Jingyuan Fang

Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China

*Corresponding author: Tel, 86-21-63200874; Fax, 86-21-63266027; E-mail, [email protected]Search for more papers by this author

Xiaoqing Tian,

Xiaoqing Tian

Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China

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Danfeng Sun,

Danfeng Sun

Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China

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Yanjie Zhang,

Yanjie Zhang

Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China

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Shuliang Zhao,

Shuliang Zhao

Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China

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Hua Xiong,

Hua Xiong

Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China

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Jingyuan Fang,

Corresponding Author

Jingyuan Fang

Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China

*Corresponding author: Tel, 86-21-63200874; Fax, 86-21-63266027; E-mail, [email protected]Search for more papers by this author

First published: 21 April 2008

https://doi.org/10.1111/j.1745-7270.2008.00405.x

Citations: 2

This work was supported by a grant from the National Basic Research Program of China 973 program (No. 2005CB522400)

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Abstract

Zinc finger protein 278 (ZNF278) is a novel Krueppel Cys2-His2-type zinc finger protein that is ubiquitously distributed in human tissues. Whether ZNF278 is related to the development of colorectal cancer is still unclear. The tran-scriptional level of ZNF278 was studied in colorectal cancer by real-time polymerase chain reaction. The results showed that ZNF278 expression was increased in 53% of colorectal cancer tissues compared to corresponding non-cancerous tissues. Thetranscriptional down-regulation of ZNF278 was detected in only three (6%) human colorectal cancer tissues compared to corresponding non-cancer tissues. No significant difference was detected in 19 (41%) pairs of samples. However, we failed to find a significant association between the up-regulation of ZNF278 transcription and age, sex, the degree of infiltration, or the tumor size of colorectal cancer. To study the function of ZNF278 in colorectal carcinogenesis, the colon cancer cell line SW1116 was stably transfected with a wild-type ZNF278 plasmid to construct an overexpression system, and was transiently transfected with the small interfering RNA of ZNF278 to construct a ZNF278 knockdown system. Cell proliferation was assessed with 3–[4, 5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide dye and a cell counter. The results show that ZNF278 promotes cell growth, and its knockdown suppresses cell proliferation. ZNF278 could be a potential proto-oncogene in colorectal cancer.

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Volume40, Issue4

April 2008

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Zinc finger protein 278, a potential oncogene in human colorectal cancer

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