High expression of osteoglycin decreases the metastatic capability of mouse hepatocarcinoma Hca-F cells to lymph nodes
Xiaonan Cui
Department of Oncology, the First Affiliate Hospital of Dalian Medical University, Dalian 116011, China
Search for more papers by this authorBo Song
Department of narcotherapy, Dalian Medical University, Dalian 116044, China
Search for more papers by this authorLi Hou
Department of narcotherapy, Dalian Medical University, Dalian 116044, China
Search for more papers by this authorZhiyi Wei
Department of Anesthesiology, The Second Affiliate Hospital of Dalian Medical University, Dalian 116027, China
Search for more papers by this authorCorresponding Author
Jianwu Tang
Department of narcotherapy, Dalian Medical University, Dalian 116044, China
*Corresponding author: Tel, 86-411-86110002; Fax, 86-411-86118866; E-mail, [email protected]Search for more papers by this authorXiaonan Cui
Department of Oncology, the First Affiliate Hospital of Dalian Medical University, Dalian 116011, China
Search for more papers by this authorBo Song
Department of narcotherapy, Dalian Medical University, Dalian 116044, China
Search for more papers by this authorLi Hou
Department of narcotherapy, Dalian Medical University, Dalian 116044, China
Search for more papers by this authorZhiyi Wei
Department of Anesthesiology, The Second Affiliate Hospital of Dalian Medical University, Dalian 116027, China
Search for more papers by this authorCorresponding Author
Jianwu Tang
Department of narcotherapy, Dalian Medical University, Dalian 116044, China
*Corresponding author: Tel, 86-411-86110002; Fax, 86-411-86118866; E-mail, [email protected]Search for more papers by this authorThis work was supported by a grant from the National Natural Science Foundation of China (No. 30500586)
Abstract
Osteoglycin, one of the matrix molecules, belongs to the small leucine-rich proteoglycan gene family and might play important roles in cell growth and differentiation and in pathological processes such as fibrosis and cancer growth. In this study, a eukaryotic expression plasmid pIRESpuro3 osteoglycin(+) was constructed and transfected into mouse hepatocarcinoma Hca-F cells to evaluate the contribution of osteoglycin to the malignant behavior of Hca-F. It was found that Hca-F cells transfected with pIRESpuro3 osteoglycin (+) showed significantly decreased potential for both migration and invasion. Furthermore, Hca-F cells transfected with osteoglycin showed decreased metastatic potential to peripheral lymph nodes. However, proliferation potential and adhesive capacity of Hca-F cells to differentprotein substrates were not influenced by osteoglycin transfection. In summary, high expression of osteoglycin decreases the metastatic capability of Hca-F to lymph nodes.
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