Gene expression profile induced by oral administration of baicalin and gardenin after focal brain ischemia in rats¹

Corresponding Author

Zhan-jun ZHANG

The Clinical School of Beijing University of Traditional Chinese Medicine, Beijing 100029, China;

Correspondence to Dr Zhan-jun ZHANG.
Phn 86-10-6287-5599, ext 6114.
Fax 86-10-6287-4049.
E-mail [email protected]Search for more papers by this author

Zhong WANG,

Zhong WANG

Xi-yuan Hospital of the Academy of Traditional Chinese Medicine, Beijing 100089, China;

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Xiao-yan ZHANG,

Xiao-yan ZHANG

Beijing Normal University, Beijing 100036, China;

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Kang YING,

Kang YING

BioStar Genechip Incorporated, Shanghai 200012, China;

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Jian-xun LIU,

Jian-xun LIU

Xi-yuan Hospital of the Academy of Traditional Chinese Medicine, Beijing 100089, China;

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Yong-yan WANG,

Yong-yan WANG

The Academy of Traditional Chinese Medicine, Beijing 100059, China

Search for more papers by this author

Zhan-jun ZHANG,

Corresponding Author

Zhan-jun ZHANG

The Clinical School of Beijing University of Traditional Chinese Medicine, Beijing 100029, China;

Correspondence to Dr Zhan-jun ZHANG.
Phn 86-10-6287-5599, ext 6114.
Fax 86-10-6287-4049.
E-mail [email protected]Search for more papers by this author

Zhong WANG,

Zhong WANG

Xi-yuan Hospital of the Academy of Traditional Chinese Medicine, Beijing 100089, China;

Search for more papers by this author

Xiao-yan ZHANG,

Xiao-yan ZHANG

Beijing Normal University, Beijing 100036, China;

Search for more papers by this author

Kang YING,

Kang YING

BioStar Genechip Incorporated, Shanghai 200012, China;

Search for more papers by this author

Jian-xun LIU,

Jian-xun LIU

Xi-yuan Hospital of the Academy of Traditional Chinese Medicine, Beijing 100089, China;

Search for more papers by this author

Yong-yan WANG,

Yong-yan WANG

The Academy of Traditional Chinese Medicine, Beijing 100059, China

Search for more papers by this author

First published: 16 February 2005

https://doi.org/10.1111/j.1745-7254.2005.00051.x

Citations: 5

Project supported by the Major State Basic Research Development Program of the People's Republic of China (No G19990554).

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Abstract

Aim: To investigate differential gene expression and the pharmacological mechanism of baicalin and gardenin in focal cerebral ischemia in rats with high-density cDNA microarray.

Methods: Rat left middle cerebral arteries were occluded and treated with either baicalin or gardenin. The pharmacological effects were investigated using the difference in infarction areas before and after treatment, which were determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining. Gene expression was demonstrated using a “Biostar40S” gene microarray. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to verify the result of the selected genes.

Results: Both baicalin and gardenin reduced the infarction areas in focal cerebral ischemia rats (P < 0.05). The differential genes were 211, 177, and 70 (upregulated or downregulated) in the model group, baicalin, and gardenin treatment groups compared with the sham-operated group, respectively. Gene expression of RpL19 and Csnk2 underwent an approximately 1.9 and 2.1-fold increase, respectively, verified by semiquantitative RT-PCR, which was the same trend as the cDNA microarray.

Conclusion: Differential gene expression with respect to the pharmacological effects of baicalin and gardenin on focal cerebral ischemia by cDNA microarray revealed a number of clues with respect to the therapeutic mechanisms of Chinese traditional medicine. In addition, the present study provided theoretical and experimental evidence that will aid future studies examining cerebral ischemia.

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Gene expression profile induced by oral administration of baicalin and gardenin after focal brain ischemia in rats¹