Mapping of a Wheat Resistance Gene to Yellow Mosaic Disease by Amplified Fragment Length Polymorphism and Simple Sequence Repeat Markers
Wei-Hua LIU
State Key Laboratory of Agrobiotechnology, China Agricultural University , Beijing 100094, China;
Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences , Beijing 100081, China
Search for more papers by this authorHuan NIE
State Key Laboratory of Agrobiotechnology, China Agricultural University , Beijing 100094, China;
Search for more papers by this authorZhen-Tian HE
Institute of Agricultural Sciences in Lixiahe District, Jiangsu Academy of Agricultural Sciences , Yangzhou 225207, China;
Search for more papers by this authorXiu-Lan CHEN
Institute of Agricultural Sciences in Lixiahe District, Jiangsu Academy of Agricultural Sciences , Yangzhou 225207, China;
Search for more papers by this authorYue-Peng HAN
Institute of Agricultural Sciences in Lixiahe District, Jiangsu Academy of Agricultural Sciences , Yangzhou 225207, China;
Search for more papers by this authorJin-Rong WANG
Institute of Agricultural Sciences in Lixiahe District, Jiangsu Academy of Agricultural Sciences , Yangzhou 225207, China;
Search for more papers by this authorXin LI
Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences , Beijing 100081, China
State Key Laboratory of Agrobiotechnology, China Agricultural University , Beijing 100094, China;
Search for more papers by this authorCheng-Gui HAN
State Key Laboratory of Agrobiotechnology, China Agricultural University , Beijing 100094, China;
Search for more papers by this authorCorresponding Author
Jia-Lin YU
State Key Laboratory of Agrobiotechnology, China Agricultural University , Beijing 100094, China;
*Author for correspondence. Tel: +86 (0)10 6273 3323; Fax: +86 (0)10 6273 2012; E-mail: 〈[email protected]〉.Search for more papers by this authorWei-Hua LIU
State Key Laboratory of Agrobiotechnology, China Agricultural University , Beijing 100094, China;
Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences , Beijing 100081, China
Search for more papers by this authorHuan NIE
State Key Laboratory of Agrobiotechnology, China Agricultural University , Beijing 100094, China;
Search for more papers by this authorZhen-Tian HE
Institute of Agricultural Sciences in Lixiahe District, Jiangsu Academy of Agricultural Sciences , Yangzhou 225207, China;
Search for more papers by this authorXiu-Lan CHEN
Institute of Agricultural Sciences in Lixiahe District, Jiangsu Academy of Agricultural Sciences , Yangzhou 225207, China;
Search for more papers by this authorYue-Peng HAN
Institute of Agricultural Sciences in Lixiahe District, Jiangsu Academy of Agricultural Sciences , Yangzhou 225207, China;
Search for more papers by this authorJin-Rong WANG
Institute of Agricultural Sciences in Lixiahe District, Jiangsu Academy of Agricultural Sciences , Yangzhou 225207, China;
Search for more papers by this authorXin LI
Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences , Beijing 100081, China
State Key Laboratory of Agrobiotechnology, China Agricultural University , Beijing 100094, China;
Search for more papers by this authorCheng-Gui HAN
State Key Laboratory of Agrobiotechnology, China Agricultural University , Beijing 100094, China;
Search for more papers by this authorCorresponding Author
Jia-Lin YU
State Key Laboratory of Agrobiotechnology, China Agricultural University , Beijing 100094, China;
*Author for correspondence. Tel: +86 (0)10 6273 3323; Fax: +86 (0)10 6273 2012; E-mail: 〈[email protected]〉.Search for more papers by this authorSupported by the National Natural Science Foundation of China (30270874).
Abstract
Abstract: Wheat (Triticum aestivum L.) yellow mosaic virus (WYMV) is transmitted by a fungal vector through soil and causes serious wheat yield losses due to yellow mosaic disease, with yellow-streaked leaves and stunted plants. In the present study, the amplified fragment length polymorphisms (AFLP) and simple sequence repeat (SSR) were used to identify the molecular linkages with the resistance gene against WYMV. Bulked segregant analysis was performed with an F2 population derived from the cross of cultivar Ningmai 9 (resistant) × cultivar Yangmai 10 (susceptible). By screening among the resistant or susceptible parents, the F2 pools and the individuals in the F2 population with 64 combined selective AFLP primers (EcoRI/MseI) or 290 reported SSR primers, a polymorphic DNA segment (approximately 120 bp) was amplified using the primer pair E2/M5, and an SSR marker (approximately 180 bp) was located on wheat chromosome 2 A using the primer Xgwm328. Analysis with MAPMAKER/Exp Version 3.0b (Whitehead institute for Biomedical Research, Cambridge, MA, USA) indicated that these two markers were dominantly associated with the resistance gene at distances of 5.4 cM or 17.6 cM, respectively. The resistance gene to WYMV derived from Ningmai 9, is temporarily named YmNM, and was mapped to wheat chromosome 2A.
(Managing editor: Li-Hui ZHAO)
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