American Journal of Reproductive Immunology

Original Article

Imbalance in Cytokines from Interleukin-1 Family – Role in Pathogenesis of Endometriosis

Corresponding Author

Justyna Sikora

Department of Immunology and Serology, Medical University of Silesia, Katowice, Poland

Correspondence

Justyna Sikora, Department of Immunology and Serology, Medical University of Silesia, Raciborska 15, 40-074 Katowice, Poland.

E-mail: [email protected]

Search for more papers by this author

Aleksandra Mielczarek-Palacz,

Aleksandra Mielczarek-Palacz

Department of Immunology and Serology, Medical University of Silesia, Katowice, Poland

Search for more papers by this author

Zdzislawa Kondera-Anasz,

Zdzislawa Kondera-Anasz

Department of Immunology and Serology, Medical University of Silesia, Katowice, Poland

Search for more papers by this author

Justyna Sikora,

Corresponding Author

Justyna Sikora

Department of Immunology and Serology, Medical University of Silesia, Katowice, Poland

Correspondence

Justyna Sikora, Department of Immunology and Serology, Medical University of Silesia, Raciborska 15, 40-074 Katowice, Poland.

E-mail: [email protected]

Search for more papers by this author

Aleksandra Mielczarek-Palacz,

Aleksandra Mielczarek-Palacz

Department of Immunology and Serology, Medical University of Silesia, Katowice, Poland

Search for more papers by this author

Zdzislawa Kondera-Anasz,

Zdzislawa Kondera-Anasz

Department of Immunology and Serology, Medical University of Silesia, Katowice, Poland

Search for more papers by this author

First published: 26 April 2012

https://doi.org/10.1111/j.1600-0897.2012.01147.x

Citations: 55

Share a link

Email
Wechat
Bluesky

Abstract

Problem

To assess whether interleukin (IL)-1beta, IL-18 and interleukin-1 converting enzyme (ICE) are involved in the pathogenesis of endometriosis.

Method of study

Peritoneal fluid (PF) was obtained from 85 women with and without endometriosis. Peritoneal macrophages were cultured and the culture media collected. IL-1beta, IL-18 and ICE levels were measured by the enzyme-linked immunosorbent assay (ELISA).

Results

Levels of IL-1beta and ICE in PF of women with endometriosis were higher than those in the control group. However, PF level of IL-18 was significantly lower in the study group than in the controls. Higher secretion of IL-1beta by peritoneal macrophages and lower IL-18 and ICE in endometriosis patients than in control were observed. Following lipopolysaccharide (LPS) stimulation, the macrophages secreted more IL-1beta, IL-18 and ICE in all groups.

Conclusions

The results pointed to impairment of the secretion of the IL-1 cytokine family in endometriosis. Invalid IL-1beta and IL-18 maturation by ICE may be an important pathogenic factor in endometriosis.

References

1 Ozkan S, Murk W, Arici A: Endometriosis and infertility: epidemiology and evidence-based treatments. Ann N Y Acad Sci 2008; 1127: 92–100.
10.1196/annals.1434.007
CAS PubMed Web of Science® Google Scholar
2 Gupta S, Goldberg JM, Aziz N, Goldberg E, Krajcir N, Agarwal A: Pathogenic mechanisms in endometriosis-associated infertility. Fertil Steril 2008; 90: 247–257.
10.1016/j.fertnstert.2008.02.093
CAS PubMed Web of Science® Google Scholar
3 Khan KN, Kitajima M, Hiraki K, Fujishita A, Sekine I, Ishimaru T, Masuzaki H: Immunopathogenesis of pelvic endometriosis: role of hepatocyte growth factor, macrophages and ovarian steroids. Am J Reprod Immunol 2008; 60: 383–404.
10.1111/j.1600-0897.2008.00643.x
CAS PubMed Web of Science® Google Scholar
4 Scholl B, Bersinger NA, Kuhn A, Mueller MD: Correlation between symptoms of pain and peritoneal fluid inflammatory cytokine concentrations in endometriosis. Gynecol Endocrinol 2009; 25: 701–706.
10.3109/09513590903159680
CAS PubMed Web of Science® Google Scholar
5 Velasco I, Acién P, Campos A, Acién MI, Ruiz-Maciá E: Interleukin-6 and other soluble factors in peritoneal fluid and endometriomas and their relation to pain and aromatase expression. J Reprod Immunol 2010; 84: 199–205.
10.1016/j.jri.2009.11.004
CAS PubMed Web of Science® Google Scholar
6 Sims JE, Smith DE: The IL-1 family: regulators of immunity. Nat Rev Immunol 2010; 10: 89–102.
10.1038/nri2691
CAS PubMed Web of Science® Google Scholar
7 Arend WP, Palmer G, Gabay C: IL-1, IL-18, and IL-33 families of cytokines. Immunol Rev 2008; 223: 20–38.
10.1111/j.1600-065X.2008.00624.x
CAS PubMed Web of Science® Google Scholar
8 Dinarello CA: Immunological and inflammatory functions of the interleukin-1 family. Annu Rev Immunol 2009; 27: 519–550.
10.1146/annurev.immunol.021908.132612
CAS PubMed Web of Science® Google Scholar
9 Boraschi D, Dinarello CA: IL-18 in autoimmunity: review. Eur Cytokine Netw 2006; 17: 224–252.
CAS PubMed Web of Science® Google Scholar
10 Li W, Yamamoto H, Kubo S, Okamura H: Modulation of innate immunity by IL-18. J Reprod Immunol 2009; 83: 101–105.
10.1016/j.jri.2009.08.005
CAS PubMed Web of Science® Google Scholar
11 Yu HB, Finlay BB: The caspase-1 inflammasome: a pilot of innate immune responses. Cell Host Microbe 2008; 4: 198–208.
10.1016/j.chom.2008.08.007
CAS PubMed Web of Science® Google Scholar
12 Akoum A, Al-Akoum M, Lemay A, Maheux R, Leboeuf M: Imbalance in the peritoneal levels of interleukin 1 and its decoy inhibitory receptor type II in endometriosis women with infertility and pelvic pain. Fertil Steril 2008; 89: 1618–1624.
10.1016/j.fertnstert.2007.06.019
CAS PubMed Web of Science® Google Scholar
13 American Society for Reproductive Medicine: Revised American Society for Reproductive Medicine Classification of Endometriosis: 1996. Fertil Steril 1997; 67: 817–821.
10.1016/S0015-0282(97)81391-X
PubMed Web of Science® Google Scholar
14 Podgaec S, Dias Junior JA, Chapron C, Oliveira RM, Baracat EC, Abrão MS: Th1 and Th2 immune responses related to pelvic endometriosis. Rev Assoc Med Bras 2010; 56: 92–98.
10.1590/S0104-42302010000100022
PubMed Web of Science® Google Scholar
15 Rogers PA, Donoghue JF, Walter LM, Girling JE: Endometrial angiogenesis, vascular maturation, and lymphangiogenesis. Reprod Sci 2009; 16: 147–151.
10.1177/1933719108325509
PubMed Web of Science® Google Scholar
16 Kharfi A, Akoum A: Soluble interleukin-1 receptor type II blocks monocyte chemotactic protein-1 secretion by U937 cells in response to peripheral blood serum of women with endometriosis. Fertil Steril 2002; 78: 836–842.
10.1016/S0015-0282(02)03335-6
PubMed Web of Science® Google Scholar
17 Kalu E, Sumar N, Giannopoulos T, Patel P, Croucher C, Sherriff E, Bansal A: Cytokine profiles in serum and peritoneal fluid from infertile women with and without endometriosis. J Obstet Gynaecol Res 2007; 33: 490–495.
10.1111/j.1447-0756.2007.00569.x
CAS PubMed Web of Science® Google Scholar
18 Keenan JA, Chen TT, Chadwell NL, Torry DS, Caudle MR: IL-1beta, TNF-alpha and IL-2 in peritoneal fluid and macrophage–conditioned media of women with endometriosis. Am J Reprod Immunol 1995; 34: 381–385.
10.1111/j.1600-0897.1995.tb00968.x
PubMed Web of Science® Google Scholar
19 Kyama CM, Overbergh L, Mihalyi A, Meuleman C, Mwenda JM, Mathieu C, D'Hooghe TM: Endometrial and peritoneal expression of aromatase, cytokines, and adhesion factors in women with endometriosis. Fertil Steril 2008; 89: 301–310.
10.1016/j.fertnstert.2007.02.057
CAS PubMed Web of Science® Google Scholar
20 Montagna P, Capellino S, Villaggio B, Remorgida V, Ragni N, Cutolo M, Ferrero S: Peritoneal fluid macrophages in endometriosis: correlation between the expression of estrogen receptors and inflammation. Fertil Steril 2008; 90: 156–164.
10.1016/j.fertnstert.2006.11.200
CAS PubMed Web of Science® Google Scholar
21 Guo L, Wei G, Zhu J, Liao W, Leonard WJ, Zhao K, Paul W: IL-1 family members and STAT activators induce cytokine production by Th2, Th17, and Th1 cells. Proc Natl Acad Sci U S A 2009; 106: 13463–13468.
10.1073/pnas.0906988106
CAS PubMed Web of Science® Google Scholar
22 Zhang X, Lin J, Qian Y, Deng L: Decreased levels of interleukin-18 in peritoneal fluid but not in serum of patients with endometriosis. Fertil Steril 2004; 81: 1229–1234.
10.1016/j.fertnstert.2003.09.074
CAS PubMed Web of Science® Google Scholar
23 Luo Q, Ning W, Wu Y, Zhu X, Jin F, Sheng J, Huang H: Altered expression of interleukin-18 in the ectopic and eutopic endometrium of women with endometriosis. J Reprod Immunol 2006; 72: 108–117.
10.1016/j.jri.2006.03.003
CAS PubMed Web of Science® Google Scholar
24 Arici A, Matalliotakis I, Goumenou A, Koumantakis G, Vassiliadis S, Mahutte NG: Altered expression of interleukin-18 in the peritoneal fluid of women with endometriosis. Fertil Steril 2003; 80: 889–894.
10.1016/S0015-0282(03)01122-1
PubMed Web of Science® Google Scholar
25 Oku H, Tsuji Y, Kashiwamura SI, Adachi S, Kubota A, Okamura H, Koyama K: Role of IL-18 in pathogenesis of endometriosis. Hum Reprod 2004; 19: 709–714.
10.1093/humrep/deh108
CAS PubMed Web of Science® Google Scholar
26 Fairbanks F, Abrao MS, Podgaec S, Dias Jr JA, de Oliveira RM, Rizzo LV: Interleukin-12 but not interleukin-18 is associated with severe endometriosis. Fertil Steril 2009; 91: 320–324.
10.1016/j.fertnstert.2007.11.060
CAS PubMed Web of Science® Google Scholar
27 McMurray RW, Suwannaroj S, Ndebele K, Jenkins JK: Differential effects of sex steroids on T and B cells: modulation of cell cycle phase distribution, apoptosis and bcl-2 protein levels. Pathobiology 2001; 69: 44–58.
10.1159/000048757
CAS PubMed Web of Science® Google Scholar
28 Glitz C, Souza CA, Rodini GP, Genro V, Bilibio JP, Senger M, Cunha-Filho JS: Peritoneal and serum interleukin-18 levels are not increased in women with minimum or mild endometriosis. Braz J Med Biol Res 2009; 42: 1039–1043.
10.1590/S0100-879X2009001100007
CAS PubMed Web of Science® Google Scholar
29 Braun DP, Ding J, Shaheen F, Willey JC, Rana N, Dmowski WP: Quantitative expression of apoptosis-regulating genes in endometrium from women with and without endometriosis. Fertil Steril 2007; 87: 263–268.
10.1016/j.fertnstert.2006.06.026
CAS PubMed Web of Science® Google Scholar
30 Agic A, Djalali S, Diedrich K, Hornung D: Apoptosis in endometriosis. Gynecol Obstet Invest 2009; 68: 217–223.
10.1159/000235871
PubMed Web of Science® Google Scholar

Citing Literature

Volume68, Issue2

August 2012

Pages 138-145

Imbalance in Cytokines from Interleukin-1 Family – Role in Pathogenesis of Endometriosis

Abstract

Problem

Method of study

Results

Conclusions

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Imbalance in Cytokines from Interleukin-1 Family – Role in Pathogenesis of Endometriosis

Abstract

Problem

Method of study

Results

Conclusions

References

Citing Literature

References

Related

Information